pGreenII 0179

Agrobacterium binary vector with kanamycin- and hygromycin-resistance genes.

Sequence Author: pGreen website

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NruI (4821) PasI (4603) EcoNI (4565) SspI (4553) PflMI (4218) AlwNI (3691) BciVI (3478) BglII (3270) StuI (3217) BspQI - SapI (3093) lac operator SacI (2814) Eco53kI (2812) BstXI (2806) SacII (2805) AleI - MslI (2804) NotI (2793) XbaI (2786) SpeI (2780) BamHI (2774) SmaI (2770) TspMI - XmaI (2768) PstI (2766) EcoRI (2756) EcoRV (2752) HindIII (2744) AccI (2730) SalI (2729) AbsI - PaeR7I - PspXI - XhoI (2723) ApaI (2718) EcoO109I (2715) PspOMI (2714) KpnI (2712) Acc65I (2708) BsaBI * (62) StyI (421) NgoMIV (524) NaeI (526) FseI (528) AanI - PsiI (546) BglII (627) LB T-DNA repeat BlpI (919) BbvCI (1005) BtgZI (1397) NdeI (1621) RsrII (1662) BfuAI - BspMI (1756) ZraI (2048) AatII (2050) BmgBI (2211) FspI (2537) BmrI (2638) pGreenII 0179 5138 bp
NruI  (4821)
1 site
T C G C G A A G C G C T
PasI  (4603)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
EcoNI  (4565)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
SspI  (4553)
1 site
A A T A T T T T A T A A
PflMI  (4218)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
AlwNI  (3691)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
BciVI  (3478)
1 site
G T A T C C ( N ) 5 N C A T A G G ( N ) 5

The 1-base overhangs produced by BciVI may be hard to ligate.
Sticky ends from different BciVI sites may not be compatible.
BglII  (3270)
2 sites
A G A T C T T C T A G A
StuI  (3217)
1 site
A G G C C T T C C G G A
BspQI  (3093)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (3093)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
SacI  (2814)
1 site
G A G C T C C T C G A G
Eco53kI  (2812)
1 site
G A G C T C C T C G A G
BstXI  (2806)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
SacII  (2805)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
AleI  (2804)
1 site
C A C N N N N G T G G T G N N N N C A C
MslI  (2804)
1 site
C A Y N N N N R T G G T R N N N N Y A C
NotI  (2793)
1 site
G C G G C C G C C G C C G G C G
XbaI  (2786)
1 site
T C T A G A A G A T C T
SpeI  (2780)
1 site
A C T A G T T G A T C A
BamHI  (2774)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
SmaI  (2770)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
TspMI  (2768)
1 site
C C C G G G G G G C C C
XmaI  (2768)
1 site
C C C G G G G G G C C C

Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
PstI  (2766)
1 site
C T G C A G G A C G T C
EcoRI  (2756)
1 site
G A A T T C C T T A A G
EcoRV  (2752)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
HindIII  (2744)
1 site
A A G C T T T T C G A A
AccI  (2730)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
SalI  (2729)
1 site
G T C G A C C A G C T G
AbsI  (2723)
1 site
C C T C G A G G G G A G C T C C
PaeR7I  (2723)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (2723)
1 site
V C T C G A G B B G A G C T C V
XhoI  (2723)
1 site
C T C G A G G A G C T C
ApaI  (2718)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
EcoO109I  (2715)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
PspOMI  (2714)
1 site
G G G C C C C C C G G G
KpnI  (2712)
1 site
G G T A C C C C A T G G
Acc65I  (2708)
1 site
G G T A C C C C A T G G
BsaBI  (62)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
StyI  (421)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
NgoMIV  (524)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
NaeI  (526)
1 site
G C C G G C