pGreen 0029

Compact Agrobacterium binary vector with a kanamycin-resistance genes for bacterial and plant transformation.

Sequence Author: pGreen website

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Plant Vectors | More Plasmid Sets
No matches
RsrII (4505) AflII (4285) AflIII - PciI (4205) MauBI (4091) SphI (4061) PsiI (3937) FseI (3919) StyI (3812) DraIII (3265) BspHI (3209) NruI (3074) EcoNI (2818) AsiSI (2733) Bpu10I - BsmBI - Esp3I (2711) PflMI (2471) NmeAIII (170) PflFI - Tth111I (273) MscI (310) KasI (389) BsaHI - NarI (390) SfoI (391) PluTI (393) NheI (616) BmtI (620) Bsu36I (632) BlpI (677) Acc65I (1031) KpnI (1035) PspOMI (1037) EcoO109I (1038) ApaI (1041) AbsI - PaeR7I - PspXI - XhoI (1046) SalI (1052) AccI (1053) HincII (1054) HindIII (1067) EcoRV (1075) EcoRI (1079) PstI (1089) TspMI - XmaI (1091) SmaI (1093) BamHI (1097) SpeI (1103) XbaI (1109) NotI (1116) AleI - MslI (1127) BstXI (1129) Eco53kI (1135) SacI (1137) lac operator StuI (1540) ApaLI (1912) AlwNI (2014) pGreen 0029 4632 bp
RsrII  (4505)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
AflII  (4285)
1 site
C T T A A G G A A T T C
AflIII  (4205)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (4205)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
MauBI  (4091)
1 site
C G C G C G C G G C G C G C G C
SphI  (4061)
1 site
G C A T G C C G T A C G
PsiI  (3937)
1 site
T T A T A A A A T A T T
FseI  (3919)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
StyI  (3812)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
DraIII  (3265)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
BspHI  (3209)
1 site
T C A T G A A G T A C T
NruI  (3074)
1 site
T C G C G A A G C G C T
EcoNI  (2818)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
AsiSI  (2733)
1 site
G C G A T C G C C G C T A G C G
Bpu10I  (2711)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BsmBI  (2711)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
Esp3I  (2711)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different Esp3I sites may not be compatible.
PflMI  (2471)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
NmeAIII  (170)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
PflFI  (273)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (273)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
MscI  (310)
1 site
T G G C C A A C C G G T
KasI  (389)
1 site
G G C G C C C C