pCAMBIA0305.1

Agrobacterium binary vector for plant transformation, with kanamycin-resistance and GUSPlus™ genes.

Sequence Author: Cambia

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HomePlasmidsPlant VectorspCAMBIA0305.1
NcoI (0) PshAI (9463) PvuII (8995) HindIII (8900) PstI - SbfI (8892) SalI (8882) XbaI (8876) BamHI (8870) SmaI (8867) KpnI - TspMI - XmaI (8865) Acc65I (8861) BanII - SacI (8859) Eco53kI (8857) EcoRI (8849) lac operator AseI (8724) LB T-DNA repeat SacII (8392) PsiI (8264) BpmI (7937) BlpI (7788) BstZ17I (6451) MreI - SgrAI (6249) AgeI (5961) EcoNI (5780) BglII (7) SpeI (216) BstBI (556) BsrGI (652) StuI (730) SnaBI (769) AleI (1554) ZraI (1610) AatII (1612) MluI (1696) DraIII (1835) AfeI (1989) 6xHis PmlI (2048) BstEII (2058) AflII (2109) MauBI (2304) PasI (3797) AclI (4391) pCAMBIA0305.1 9662 bp
NcoI  (0)
1 site		 C C A T G G G G T A C C
PshAI  (9463)
1 site		 G A C N N N N G T C C T G N N N N C A G
PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
PvuII  (8995)
1 site		 C A G C T G G T C G A C
HindIII  (8900)
1 site		 A A G C T T T T C G A A
PstI  (8892)
1 site		 C T G C A G G A C G T C
SbfI  (8892)
1 site		 C C T G C A G G G G A C G T C C
SalI  (8882)
1 site		 G T C G A C C A G C T G
XbaI  (8876)
1 site		 T C T