pPZP121
Agrobacterium binary vector for plant transformation, with chloramphenicol- and gentamycin-resistance genes. The MCS is reversed in pPZP122.
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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SmaI can be used at 37°C for brief incubations. |
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Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present. |
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Sticky ends from different BanII sites may not be compatible. |
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Sticky ends from different BstXI sites may not be compatible. |
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| * Blocked by Dam methylation. BclI is typically used at 50-55°C, but is 50% active at 37°C. |
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Efficient cleavage requires at least two copies of the BpmI recognition sequence. Sticky ends from different BpmI sites may not be compatible.After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.BpmI quickly loses activity at 37°C. |
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BsiWI is typically used at 55°C, but is 50% active at 37°C. |
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| * Blocked by Dam methylation. |
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| * Blocked by Dam methylation. |
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The 1-base overhangs produced by EcoNI may be hard to ligate.Sticky ends from different EcoNI sites may not be compatible. |
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Sticky ends from different BsaI sites may not be compatible.BsaI can be used between 37°C and 50°C. |
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Efficient cleavage requires at least two copies of the NarI recognition sequence. |
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Efficient cleavage requires at least two copies of the PluTI recognition sequence. |
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| pVS1 RepA 2245 .. 3318 = 1074 bp 357 amino acids = 39.9 kDa Product: replication protein from Pseudomonas plasmid pVS1 |
| pVS1 RepA 2245 .. 3318 = 1074 bp 357 amino acids = 39.9 kDa Product: replication protein from Pseudomonas plasmid pVS1 |
| CaMV 35S promoter (enhanced) 7218 .. 7896 = 679 bp cauliflower mosaic virus 35S promoter with a duplicated enhancer region |
| CaMV 35S promoter (enhanced) 7218 .. 7896 = 679 bp cauliflower mosaic virus 35S promoter with a duplicated enhancer region |
| CmR 5107 .. 5766 = 660 bp 219 amino acids = 25.7 kDa Product: chloramphenicol acetyltransferase confers resistance to chloramphenicol |
| CmR 5107 .. 5766 = 660 bp 219 amino acids = 25.7 kDa Product: chloramphenicol acetyltransferase confers resistance to chloramphenicol |
| pVS1 StaA 1187 .. 1816 = 630 bp 209 amino acids = 22.1 kDa Product: stability protein from Pseudomonas plasmid pVS1 |
| pVS1 StaA 1187 .. 1816 = 630 bp 209 amino acids = 22.1 kDa Product: stability protein from Pseudomonas plasmid pVS1 |
| ori 4248 .. 4836 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
| ori 4248 .. 4836 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
| GmR 6625 .. 7158 = 534 bp 177 amino acids = 19.4 kDa Product: gentamycin acetyltransferase confers resistance to gentamycin |
| GmR 6625 .. 7158 = 534 bp 177 amino acids = 19.4 kDa Product: gentamycin acetyltransferase confers resistance to gentamycin |
| lacZα 8197 .. 8430 = 234 bp 77 amino acids = 8.6 kDa Product: LacZα fragment of β-galactosidase |
| lacZα 8197 .. 8430 = 234 bp 77 amino acids = 8.6 kDa Product: LacZα fragment of β-galactosidase |
| pVS1 oriV 3384 .. 3578 = 195 bp origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000) |
| pVS1 oriV 3384 .. 3578 = 195 bp origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000) |
| CaMV poly(A) signal 6416 .. 6590 = 175 bp cauliflower mosaic virus polyadenylation signal |
| CaMV poly(A) signal 6416 .. 6590 = 175 bp cauliflower mosaic virus polyadenylation signal |
| bom 3922 .. 4062 = 141 bp basis of mobility region from pBR322 |
| bom 3922 .. 4062 = 141 bp basis of mobility region from pBR322 |
| lac promoter 8123 .. 8153 = 31 bp 3 segments Segment 1: -35 8123 .. 8128 = 6 bp promoter for the E. coli lac operon |
| lac promoter 8123 .. 8153 = 31 bp 3 segments Segment 2: 8129 .. 8146 = 18 bp promoter for the E. coli lac operon |
| lac promoter 8123 .. 8153 = 31 bp 3 segments Segment 3: -10 8147 .. 8153 = 7 bp promoter for the E. coli lac operon |
| lac promoter 8123 .. 8153 = 31 bp 3 segments promoter for the E. coli lac operon |
| LB T-DNA repeat 6294 .. 6318 = 25 bp left border repeat from nopaline C58 T-DNA |
| LB T-DNA repeat 6294 .. 6318 = 25 bp left border repeat from nopaline C58 T-DNA |
| RB T-DNA repeat 8490 .. 8514 = 25 bp right border repeat from nopaline C58 T-DNA |
| RB T-DNA repeat 8490 .. 8514 = 25 |