pPZP121

Agrobacterium binary vector for plant transformation, with chloramphenicol- and gentamycin-resistance genes. The MCS is reversed in pPZP122.
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PmeI (8475) HindIII (8262) PstI - SbfI (8254) SalI (8244) XbaI (8238) BamHI (8232) SmaI (8229) KpnI - TspMI - XmaI (8227) Acc65I (8223) BanII - SacI (8221) Eco53kI (8219) EcoRI (8211) lac operator BstXI (7968) NcoI (7155) PmlI (6522) BclI * (6056) BspEI (5552) BpmI (5432) SspI (5240) HpaI (5033) NsiI (4913) BsiWI (2860) NheI (2976) BmtI (2980) BspDI * - ClaI * (3056) EcoNI (3289) BsaI (3379) AgeI (3470) KasI (3761) NarI (3762) SfoI (3763) PluTI (3765) BstZ17I (3960) pPZP121 8627 bp
PmeI  (8475)
1 site
G T T T A A A C C A A A T T T G
HindIII  (8262)
1 site
A A G C T T T T C G A A
PstI  (8254)
1 site
C T G C A G G A C G T C
SbfI  (8254)
1 site
C C T G C A G G G G A C G T C C
SalI  (8244)
1 site
G T C G A C C A G C T G
XbaI  (8238)
1 site
T C T A G A A G A T C T
BamHI  (8232)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
SmaI  (8229)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
KpnI  (8227)
1 site
G G T A C C C C A T G G
TspMI  (8227)
1 site
C C C G G G G G G C C C
XmaI  (8227)
1 site
C C C G G G G G G C C C

Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
Acc65I  (8223)
1 site
G G T A C C C C A T G G
BanII  (8221)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
SacI  (8221)
1 site
G A G C T C C T C G A G
Eco53kI  (8219)
1 site
G A G C T C C T C G A G
EcoRI  (8211)
1 site
G A A T T C C T T A A G
BstXI  (7968)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
NcoI  (7155)
1 site
C C A T G G G G T A C C
PmlI  (6522)
1 site
C A C G T G G T G C A C
BclI  (6056)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BspEI  (5552)
1 site
T C C G G A A G G C C T
BpmI  (5432)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
SspI  (5240)
1 site
A A T A T T T T A T A A
HpaI  (5033)
1 site
G T T A A C C A A T T G
NsiI  (4913)
1 site
A T G C A T T A C G T A
BsiWI  (2860)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
NheI  (2976)
1 site
G C T A G C C G A T C G
BmtI  (2980)
1 site
G C T A G C C G A T C G
BspDI  (3056)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (3056)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
EcoNI  (3289)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
BsaI  (3379)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AgeI  (3470)
1 site
A C C G G T T G G C C A
KasI &#