pCAMBIA1200
Agrobacterium binary vector for plant transformation, with hygromycin- and chloramphenicol-resistance genes.
Sequence Author: Cambia
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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Sticky ends from different BanII sites may not be compatible. |
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Sticky ends from different BstXI sites may not be compatible. |
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EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage. |
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Efficient cleavage requires at least two copies of the RsrII recognition sequence. Sticky ends from different RsrII sites may not be compatible.For full activity, add fresh DTT. |
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| * Blocked by Dam methylation. BclI is typically used at 50-55°C, but is 50% active at 37°C. |
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| * Blocked by Dam methylation. |
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BsiWI is typically used at 55°C, but is 50% active at 37°C. |
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| * Blocked by Dam methylation. |
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| * Blocked by Dam methylation. |
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The 1-base overhangs produced by EcoNI may be hard to ligate.Sticky ends from different EcoNI sites may not be compatible. |
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Sticky ends from different BsaI sites may not be compatible.BsaI can be used between 37°C and 50°C. |
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Efficient cleavage requires at least two copies of the NarI recognition sequence. |
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Efficient cleavage requires at least two copies of the PluTI recognition sequence. |
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| pVS1 RepA 2277 .. 3350 = 1074 bp 357 amino acids = 39.9 kDa Product: replication protein from Pseudomonas plasmid pVS1 |
| pVS1 RepA 2277 .. 3350 = 1074 bp 357 amino acids = 39.9 kDa Product: replication protein from Pseudomonas plasmid pVS1 |
| HygR 6642 .. 7667 = 1026 bp 341 amino acids = 38.0 kDa Product: hygromycin B phosphotransferase confers resistance to hygromycin |
| HygR 6642 .. 7667 = 1026 bp 341 amino acids = 38.0 kDa Product: hygromycin B phosphotransferase confers resistance to hygromycin |
| CaMV 35S promoter (enhanced) 7735 .. 8412 = 678 bp cauliflower mosaic virus 35S promoter with a duplicated enhancer region |
| CaMV 35S promoter (enhanced) 7735 .. 8412 = 678 bp cauliflower mosaic virus 35S promoter with a duplicated enhancer region |
| CmR 5139 .. 5798 = 660 bp 219 amino acids = 25.7 kDa Product: chloramphenicol acetyltransferase confers resistance to chloramphenicol |
| CmR 5139 .. 5798 = 660 bp 219 amino acids = 25.7 kDa Product: chloramphenicol acetyltransferase confers resistance to chloramphenicol |
| pVS1 StaA 1219 .. 1848 = 630 bp 209 amino acids = 22.1 kDa Product: stability protein from Pseudomonas plasmid pVS1 |
| pVS1 StaA 1219 .. 1848 = 630 bp 209 amino acids = 22.1 kDa Product: stability protein from Pseudomonas plasmid pVS1 |
| ori 4280 .. 4868 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
| ori 4280 .. 4868 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
| lacZα 8713 .. 8946 = 234 bp 77 amino acids = 8.6 kDa Product: LacZα fragment of β-galactosidase |
| lacZα 8713 .. 8946 = 234 bp 77 amino acids = 8.6 kDa Product: LacZα fragment of β-galactosidase |
| pVS1 oriV 3416 .. 3610 = 195 bp origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000) |
| pVS1 oriV 3416 .. 3610 = 195 bp origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000) |
| CaMV poly(A) signal 6428 .. 6602 = 175 bp cauliflower mosaic virus polyadenylation signal |
| CaMV poly(A) signal 6428 .. 6602 = 175 bp cauliflower mosaic virus polyadenylation signal |
| bom 3954 .. 4094 = 141 bp basis of mobility region from pBR322 |
| bom 3954 .. 4094 = 141 bp basis of mobility region from pBR322 |
| lac promoter 8639 .. 8669 = 31 bp 3 segments Segment 1: -35 8639 .. 8644 = 6 bp promoter for the E. coli lac operon |
| lac promoter 8639 .. 8669 = 31 bp 3 segments Segment 2: 8645 .. 8662 = 18 bp promoter for the E. coli lac operon |
| lac promoter 8639 .. 8669 = 31 bp 3 segments Segment 3: -10 8663 .. 8669 = 7 bp promoter for the E. coli lac operon |
| lac promoter 8639 .. 8669 = 31 bp 3 segments promoter for the E. coli lac operon |
| LB T-DNA repeat 6326 .. 6350 = 25 bp left border repeat from nopaline C58 T-DNA |
| LB T-DNA repeat 6326 .. 6350 = 25 bp left border repeat from nopaline C58 T-DNA |
| RB T-DNA repeat 9006 .. 9030 = 25 bp right border repeat from nopaline C58 T-DNA |
| RB T-DNA repeat 9006 .. 9030 = 25 bp right border repeat from nopaline C58 T-DNA |
| lac operator 8677 .. 8693 = 17 bp The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG). |
| lac operator 8677 .. 8693 = 17 bp The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG). |
| MCS 8727 .. 8783 = 57 bp pUC18/19 multiple cloning site |
| MCS 8727 .. 8783 = 57 bp pUC18/19 multiple cloning site |
| M13 rev 8701 .. 8717 = 17 bp common sequencing primer, one of multiple similar variants |
| M13 rev 8701 .. 8717 = 17 bp common sequencing primer, one of multiple similar variants |