pCAMBIA1200
Agrobacterium binary vector for plant transformation, with hygromycin- and chloramphenicol-resistance genes.
Sequence Author: Cambia
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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Sticky ends from different BanII sites may not be compatible. |
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Sticky ends from different BstXI sites may not be compatible. |
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EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage. |
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Efficient cleavage requires at least two copies of the RsrII recognition sequence. Sticky ends from different RsrII sites may not be compatible.For full activity, add fresh DTT. |
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| * Blocked by Dam methylation. BclI is typically used at 50-55°C, but is 50% active at 37°C. |
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| * Blocked by Dam methylation. |
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BsiWI is typically used at 55°C, but is 50% active at 37°C. |
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| * Blocked by Dam methylation. |
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| * Blocked by Dam methylation. |
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The 1-base overhangs produced by EcoNI may be hard to ligate.Sticky ends from different EcoNI sites may not be compatible. |
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Sticky ends from different BsaI sites may not be compatible.BsaI can be used between 37°C and 50°C. |
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