pCAMBIA2201

Agrobacterium binary vector for plant transformation, with kanamycin- and chloramphenicol-resistance and GUS genes.

Sequence Author: Cambia

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lacZα HindIII (11,024) PstI - SbfI (11,016) SalI (11,006) XbaI (11,000) BamHI (10,994) SmaI (10,991) KpnI - TspMI - XmaI (10,989) Acc65I (10,985) SacI (10,983) Eco53kI (10,981) EcoRI (10,973) lac operator lac promoter BstXI (10,730) NeoR/KanR RsrII (9267) PspXI (9065) HpaI (7527) BstZ17I (6454) AgeI (5964) AhdI (216) SnaBI (600) BspHI (1100) BstBI (1309) 6xHis DraIII - PmlI (2048) BstEII (2061) AflII (2112) MauBI (2307) RB T-DNA repeat BsiWI (5354) EcoNI (5783) BsaI (5873) pCAMBIA2201 11,786 bp
HindIII  (11,024)
1 site
A A G C T T T T C G A A
PstI  (11,016)
1 site
C T G C A G G A C G T C
SbfI  (11,016)
1 site
C C T G C A G G G G A C G T C C
SalI  (11,006)
1 site
G T C G A C C A G C T G
XbaI  (11,000)
1 site
T C T A G A A G A T C T
BamHI  (10,994)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
SmaI  (10,991)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
KpnI  (10,989)
1 site
G G T A C C C C A T G G
TspMI  (10,989)
1 site
C C C G G G G G G C C C
XmaI  (10,989)
1 site
C C C G G G G G G C C C

Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
Acc65I  (10,985)
1 site
G G T A C C C C A T G G
SacI  (10,983)
1 site
G A G C T C C T C G A G
Eco53kI  (10,981)
1 site
G A G C T C C T C G A G
EcoRI  (10,973)
1 site
G A A T T C C T T A A G
BstXI  (10,730)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
RsrII  (9267)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
PspXI  (9065)
1 site
V C T C G A G B B G A G C T C V
HpaI  (7527)
1 site
G T T A A C C A A T T G
BstZ17I  (6454)
1 site
G T A T A C C A T A T G
AgeI  (5964)
1 site
A C C G G T T G G C C A
AhdI  (216)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
SnaBI  (600)
1 site
T A C G T A A T G C A T
BspHI  (1100)
1 site
T C A T G A A G T A C T
BstBI  (1309)
1 site
T T C G A A A A G C T T
DraIII  (2048)
1 site
C A C N N N G T G G T G