pCAMBIA2201
Agrobacterium binary vector for plant transformation, with kanamycin- and chloramphenicol-resistance and GUS genes.
Sequence Author: Cambia
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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SmaI can be used at 37°C for brief incubations. |
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Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present. |
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Sticky ends from different BstXI sites may not be compatible. |
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Efficient cleavage requires at least two copies of the RsrII recognition sequence. Sticky ends from different RsrII sites may not be compatible.For full activity, add fresh DTT. |
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The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible. |
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Sticky ends from different DraIII sites may not be compatible. |
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Sticky ends from different BstEII sites may not be compatible.BstEII is typically used at 60°C, but is 50% active at 37°C. |
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BsiWI is typically used at 55°C, but is 50% active at 37°C. |
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The 1-base overhangs produced by EcoNI may be hard to ligate.Sticky ends from different EcoNI sites may not be compatible. |
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