pEarleyGate 204
Gateway®-compatible plant transformation vector with an N-terminal AcV5 tag.
Sequence Author: Pikaard Lab
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| * Blocked by Dam methylation. BclI is typically used at 50-55°C, but is 50% active at 37°C. |
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EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage. |
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Efficient cleavage requires at least two copies of the PluTI recognition sequence. |
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Efficient cleavage requires at least two copies of the NarI recognition sequence. |
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The 1-base overhangs produced by EcoNI may be hard to ligate.Sticky ends from different EcoNI sites may not be compatible. |
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BsiWI is typically used at 55°C, but is 50% active at 37°C. |
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Efficient cleavage requires at least two copies of the PaqCI recognition sequence. Sticky ends from different PaqCI sites may not be compatible.Cleavage can be improved with PaqCI Activator. |
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PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations. |
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PaeR7I does not recognize the sequence CTCTCGAG. |
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Sticky ends from different BstXI sites may not be compatible. |
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