pGreenII

Improved compact Agrobacterium binary vector with a kanamycin-resistance gene. Also known as pGreenII 0000.

Sequence Author: pGreen website

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HomePlasmidsPlant VectorspGreenII
StyI (330) BsaBI * (3275) DraIII (3087) BsrDI (3063) BspHI (3031) NruI (2896) TsoI (2714) PasI (2678) EcoNI (2640) SspI (2628) AsiSI (2555) Bpu10I - BsmBI (2533) PflMI (2293) BspHI (2070) AcuI (1898) AlwNI (1766) ApaLI (1664) PspFI (1658) BseYI (1654) NgoMIV (433) NaeI (435) FseI (437) AanI - AanI - PsiI (455) BglII (536) HpaI (568) FspI (612) BmrI (713) Acc65I (783) KpnI (787) PspOMI (789) EcoO109I (790) ApaI (793) AbsI - PaeR7I - PspXI - XhoI (798) SalI (804) AccI (805) BspDI - ClaI (814) HindIII (819) EcoRV (827) EcoRI (831) PstI (841) TspMI - XmaI (843) SmaI (845) BamHI (849) SpeI (855) XbaI (861) NotI (868) BpmI (873) AleI - MslI (879) SacII (880) BstXI (881) Eco53kI (887) SacI (889) lac operator BspQI - SapI (1168) StuI (1292) BglII (1345) DrdI (1458) BciVI (1553) pGreenII 3304 bp
StyI  (330)
1 site		 C C W W G G G G W W C C
Sticky ends from different StyI sites may not be compatible.
BsaBI  (3275)
1 site		 G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
DraIII  (3087)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
BsrDI  (3063)
1 site		 G C A A T G N N C G T T A C
Sticky ends from different BsrDI sites may not be compatible.
BspHI  (3031)
2 sites		 T C A T G A A G T A C T
NruI  (2896)
1 site		 T C G C G A A G C G C T
TsoI  (2714)
1 site		 T A R C C A ( N ) 9 N N A T Y G G T ( N ) 9
Sticky ends from different TsoI sites may not be compatible.
After cleavage, TsoI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
PasI  (2678)
1 site		 C C C W G G G G G G W C C C
Sticky ends from different PasI sites may not be compatible.
EcoNI  (2640)
1 site		 C C T N N N N N A G G G G A N N N N N T C C
The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
SspI  (2628)
1 site		 A A T A T T T T A T A A
AsiSI  (2555)
1 site		 G C G A T C G C C G C T A G C G
Bpu10I  (2533)
1 site		 C C T N A G C G G A N T C G
Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BsmBI  (2533)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
PflMI  (2293)
1 site		 C C A N N N N N T G G G G T N N N N N A C C
Sticky ends from different PflMI sites may not be compatible.
BspHI  (2070)
2 sites		 T C A T G A A G T A C T
AcuI  (1898)
1 site		 C T G A A G ( N ) 14 N N G A C T T C ( N ) 14
Cleavage may be enhanced when more than one copy of the AcuI recognition sequence is present.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
AlwNI  (1766)
1 site		 C A G N N N C T G G T C N N N G A C
Sticky ends from different AlwNI sites may not be compatible.
ApaLI  (1664)
1 site		 G T G C A C C A C G T G
PspFI  (1658)
1 site		 C C C A G C G G G T C G
BseYI  (1654)
1 site		 C C C A G C G G G T C G
After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
NgoMIV  (433)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
NaeI  (435)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NaeI recognition sequence.
FseI  (437)
1 site		 G G C C G G C C C C G G C C G G
FseI gradually loses activity when stored at -20°C.
AanI  (455)
1 site		 T T A T A A A A T A T T
AanI  (455)
1 site		 T T A T A A A A T A T T
PsiI  (455)
1 site		 T T A T A A A A T A T T
BglII  (536)
2 sites		 A G A T C T T C T A G A
HpaI  (568)
1 site		 G T T A A C C A A T T G
FspI  (612)
1 site		 T G C G C A A C G C G T
BmrI  (713)
1 site		 A C T G G G ( N ) 4 N T G A C C C ( N ) 4
The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the absence of magnesium.
Acc65I  (783)
1 site		 G G T A C C C C A T G G
KpnI  (787)
1 site		 G G T A C C C C A T G G
PspOMI  (789)
1 site		 G G G C C C C C C G G G
EcoO109I  (790)
1 site		 R G G N C C Y Y C C N G G R
Sticky ends from different EcoO109I sites may not be compatible.
ApaI  (793)
1 site		 G G G C C C C C C G G G
ApaI can be used between 25°C and 37°C.
AbsI  (798)
1 site		 C C T C G A G G G G A G C T C C
PaeR7I  (798)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (798)
1 site		 V C T C G A G B B G A G C T C V
XhoI  (798)
1 site		 C T C G A G G A G C T C
SalI  (804)
1 site		 G T C G A C C A G C T G
AccI  (805)
1 site		 G T M K A C C A K M T G
Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
BspDI  (814)
1 site		 A T C G A T T A G C T A
ClaI  (814)
1 site		 A T C G A T T A G C T A
HindIII  (819)
1 site		 A A G C T T T T C G A A
EcoRV  (827)
1 site		 G A T A T C C T A T A G
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
EcoRI  (831)
1 site		 G A A T T C C T T A A G
PstI  (841)
1 site		 C T G C A G G A C G T C
TspMI  (843)
1 site		 C C C G G G G G G C C C
XmaI  (843)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (845)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
BamHI  (849)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
SpeI  (855)
1 site		 A C T A G T T G A T C A
XbaI  (861)
1 site		 T C T A G A A G A T C T
NotI  (868)
1 site		 G C G G C C G C C G C C G G C G
BpmI  (873)
1 site		 C T G G A G ( N ) 14 N N G A C C T C ( N ) 14
Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
AleI  (879)
1 site		 C A C N N N N G T G G T G N N N N C A C
MslI  (879)
1 site		 C A Y N N N N R T G G T R N N N N Y A C
SacII  (880)
1 site		 C C G C G G G G C G C C
Efficient cleavage requires at least two copies of the SacII recognition sequence.
BstXI  (881)
1 site		 C C A N N N N N N T G G G G T N N N N N N A C C
Sticky ends from different BstXI sites may not be compatible.
Eco53kI  (887)
1 site		 G A G C T C C T C G A G
SacI  (889)
1 site		 G A G C T C C T C G A G
BspQI  (1168)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different BspQI sites may not be compatible.
SapI  (1168)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
StuI  (1292)
1 site		 A G G C C T T C C G G A
BglII  (1345)
2 sites		 A G A T C T T C T A G A
DrdI  (1458)
1 site		 G A C N N N N N N G T C C T G N N N N N N C A G
Sticky ends from different DrdI sites may not be compatible.
BciVI  (1553)
1 site		 G T A T C C ( N ) 5 N C A T A G G ( N ) 5
The 1-base overhangs produced by BciVI may be hard to ligate.
Sticky ends from different BciVI sites may not be compatible.
KanR
2170 .. 2985  =  816 bp
271 amino acids  =  31.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes
KanR
2170 .. 2985  =  816 bp
271 amino acids  =  31.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes
lacZα
296 .. 946  =  651 bp
216 amino acids  =  23.7 kDa
Product: lacZα fragment of β-galactosidase
lacZα
296 .. 946  =  651 bp
216 amino acids  =  23.7 kDa
Product: lacZα fragment of β-galactosidase
ori
1411 .. 1999  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
1411 .. 1999  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
lac promoter
990 .. 1021  =  32 bp
3 segments
   Segment 3:  -10  
   990 .. 996  =  7 bp
promoter for the E. coli lac operon
lac promoter
990 .. 1021  =  32 bp
3 segments
   Segment 2:  
   997 .. 1015  =  19 bp
promoter for the E. coli lac operon
lac promoter
990 .. 1021  =  32 bp
3 segments
   Segment 1:  -35  
   1016 .. 1021  =  6 bp
promoter for the E. coli lac operon
lac promoter
990 .. 1021  =  32 bp
3 segments
promoter for the E. coli lac operon
RB T-DNA repeat
1296 .. 1320  =  25 bp
right border repeat from nopaline C58 T-DNA
RB T-DNA repeat
1296 .. 1320  =  25 bp
right border repeat from nopaline C58 T-DNA
lac operator
966 .. 982  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
966 .. 982  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
ORF:  1332 .. 1736  =  405 bp
ORF:  134 amino acids  =  15.0 kDa
ORF:  2148 .. 2375  =  228 bp
ORF:  75 amino acids  =  8.8 kDa
ORF:  296 .. 946  =  651 bp
ORF:  216 amino acids  =  23.7 kDa
ORF:  2170 .. 2985  =  816 bp
ORF:  271 amino acids  =  31.0 kDa
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pEarleyGate 100
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