pPZP211
Agrobacterium binary vector for plant transformation, with spectinomycin- and kanamycin-resistance genes. The MCS is reversed in pPZP212.
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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SmaI can be used at 37°C for brief incubations. |
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Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present. |
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Sticky ends from different BstXI sites may not be compatible. |
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EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage. |
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Efficient cleavage requires at least two copies of the RsrII recognition sequence. Sticky ends from different RsrII sites may not be compatible.For full activity, add fresh DTT. |
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Sticky ends from different BstEII sites may not be compatible.BstEII is typically used at 60°C, but is 50% active at 37°C. |
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Sticky ends from different BlpI sites may not be compatible. |
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Sticky ends from different PasI sites may not be compatible. |
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BsiWI is typically used at 55°C, but is 50% active at 37°C. |
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The 1-base overhangs produced by EcoNI may be hard to ligate.Sticky ends from different EcoNI sites may not be compatible. |
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Sticky ends from different BsaI sites may not be compatible.BsaI can be used between 37°C and 50°C. |
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pVS1 RepA 2245 .. 3318 = 1074 bp 357 amino acids = 39.9 kDa Product: replication protein from Pseudomonas plasmid pVS1 |
pVS1 RepA 2245 .. 3318 = 1074 bp 357 amino acids = 39.9 kDa Product: replication protein from Pseudomonas plasmid pVS1 |
NeoR/KanR 6749 .. 7546 = 798 bp 265 amino acids = 29.1 kDa Product: aminoglycoside phosphotransferase from Tn5 confers resistance to neomycin, kanamycin, and G418 (Geneticin®) |
NeoR/KanR 6749 .. 7546 = 798 bp 265 amino acids = 29.1 kDa Product: aminoglycoside phosphotransferase from Tn5 confers resistance to neomycin, kanamycin, and G418 (Geneticin®) |
SmR 5080 .. 5871 = 792 bp 263 amino acids = 29.2 kDa Product: aminoglycoside adenylyltransferase confers resistance to spectinomycin and streptomycin |
SmR 5080 .. 5871 = 792 bp 263 amino acids = 29.2 kDa Product: aminoglycoside adenylyltransferase confers resistance to spectinomycin and streptomycin |
CaMV 35S promoter (enhanced) 7606 .. 8284 = 679 bp cauliflower mosaic virus 35S promoter with a duplicated enhancer region |
CaMV 35S promoter (enhanced) 7606 .. 8284 = 679 bp cauliflower mosaic virus 35S promoter with a duplicated enhancer region |
pVS1 StaA 1187 .. 1816 = 630 bp 209 amino acids = 22.1 kDa Product: stability protein from Pseudomonas plasmid pVS1 |
pVS1 StaA 1187 .. 1816 = 630 bp 209 amino acids = 22.1 kDa Product: stability protein from Pseudomonas plasmid pVS1 |
ori 4248 .. 4836 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
ori 4248 .. 4836 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
lacZα 8585 .. 8818 = 234 bp 77 amino acids = 8.6 kDa Product: LacZα fragment of β-galactosidase |
lacZα 8585 .. 8818 = 234 bp 77 amino acids = 8.6 kDa Product: LacZα fragment of β-galactosidase |
pVS1 oriV 3384 .. 3578 = 195 bp origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000) |
pVS1 oriV 3384 .. 3578 = 195 bp origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000) |
CaMV poly(A) signal 6521 .. 6695 = 175 bp cauliflower mosaic virus polyadenylation signal |
CaMV poly(A) signal 6521 .. 6695 = 175 bp cauliflower mosaic virus polyadenylation signal |
bom 3922 .. 4062 = 141 bp basis of mobility region from pBR322 |
bom 3922 .. 4062 = 141 bp basis of mobility region from pBR322 |
lac promoter 8511 .. 8541 = 31 bp 3 segments Segment 1: -35 8511 .. 8516 = 6 bp promoter for the E. coli lac operon |
lac promoter 8511 .. 8541 = 31 bp 3 segments Segment 2: 8517 .. 8534 = 18 bp promoter for the E. coli lac operon |
lac promoter 8511 .. 8541 = 31 bp 3 segments Segment 3: -10 8535 .. 8541 = 7 bp promoter for the E. coli lac operon |
lac promoter 8511 .. 8541 = 31 bp 3 segments promoter for the E. coli lac operon |
LB T-DNA repeat 6399 .. 6423 = 25 bp left border repeat from nopaline C58 T-DNA |
LB T-DNA repeat 6399 .. 6423 = 25 bp left border repeat from nopaline C58 T-DNA |
RB T-DNA repeat 8878 .. 8902 = 25 bp right border repeat from nopaline C58 T-DNA |
RB T-DNA repeat 8878 .. 8902 = 25 bp right border repeat from nopaline C58 T-DNA |
lac operator 8549 .. 8565 = 17 bp The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG). |
lac operator 8549 .. 8565 = 17 bp The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG). |
MCS 8599 .. 8655 = 57 bp pUC18 multiple cloning site |
MCS 8599 .. 8655 = 57 bp pUC18 multiple cloning site |
M13 rev 8573 .. 8589 = 17 bp common sequencing primer, one of multiple similar variants |
M13 rev 8573 .. 8589 = 17 bp common sequencing primer, one of multiple similar variants |
M13 fwd 8659 .. 8675 = 17 bp common sequencing primer, one of multiple similar variants |
M13 fwd 8659 .. 8675 = 17 bp common sequencing primer, one of multiple similar variants |
ORF: 202 .. 888 = 687 bp ORF: 228 amino acids = 25.1 kDa |
ORF: 2143 .. 3318 = 1176 bp ORF: 391 amino acids = 43.9 kDa |
ORF: 5449 .. 5832 = 384 bp ORF: 127 amino acids = 14.6 kDa |
ORF: 6136 .. 6477 = 342 bp ORF: 113 amino acids = 12.1 kDa |
ORF: 1187 .. 1816 = 630 bp ORF: 209 amino acids = 22.1 kDa |
ORF: 5294 .. 5572 = 279 bp ORF: 92 amino acids = 9.4 kDa |
ORF: 5582 .. 5839 = 258 bp ORF: 85 amino acids = 9.1 kDa |
ORF: 6980 .. 7234 = 255 bp ORF: 84 amino acids = 9.7 kDa |
ORF: 8585 .. 8818 = 234 bp ORF: 77 amino acids = 8.6 kDa |
ORF: 6024 .. 6266 = 243 bp ORF: 80 amino acids = 9.1 kDa |
ORF: 925 .. 1239 = 315 bp ORF: 104 amino acids = 12.0 kDa |
ORF: 2113 .. 2685 = 573 bp ORF: 190 amino acids = 20.6 kDa |
ORF: 5080 .. 6012 = 933 bp ORF: 310 amino acids = 34.5 kDa |
ORF: 6985 .. 7371 = 387 bp ORF: 128 amino acids = 14.6 kDa |
ORF: 7711 .. 8028 = 318 bp ORF: 105 amino acids = 11.6 kDa |
ORF: 8038 .. 8496 = 459 bp ORF: 152 amino acids = 16.9 kDa |
ORF: 234 .. 584 = 351 bp ORF: 116 amino acids = 12.6 kDa |
ORF: 2091 .. 2348 = 258 bp ORF: 85 amino acids = 9.6 kDa |
ORF: 653 .. 925 = 273 bp ORF: 90 amino acids = 9.9 kDa |
ORF: 6749 .. 7546 = 798 bp ORF: 265 amino acids = 29.1 kDa |
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