pCAMBIA0105.1R

Agrobacterium binary vector for plant transformation, with spectinomycin-resistance and GUSPlus™ genes.

Sequence Author: Cambia

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HomePlasmidsPlant VectorspCAMBIA0105.1R
NcoI (0) PshAI (9834) PvuII (9366) ApaI (9246) PspOMI (9242) XbaI (9236) PaeR7I - PspXI - XhoI (9224) PstI (9200) EcoRI (9191) BamHI (9160) SacI (9158) Eco53kI (9156) KpnI (9152) Acc65I (9148) HindIII (9142) lac operator AseI (9014) LB T-DNA repeat SacII (8682) BpmI (8113) BlpI (8051) HpaI (7524) BstZ17I (6451) AgeI (5961) EcoNI (5780) BglII (7) SspI (137) BstBI (556) BsrGI (652) StuI (730) SnaBI (769) Bpu10I (915) AleI (1554) ZraI (1610) AatII (1612) MluI (1696) BspHI * (1729) AfeI (1989) 6xHis PmlI (2048) AflII (2109) MauBI (2304) PasI (3797) AclI (4391) pCAMBIA0105.1R 10,033 bp
NcoI  (0)
1 site		 C C A T G G G G T A C C
PshAI  (9834)
1 site		 G A C N N N N G T C C T G N N N N C A G
PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
PvuII  (9366)
1 site		 C A G C T G G T C G A C
ApaI  (9246)
1 site		 G G G C C C C C C G G G
ApaI can be used between 25°C and 37°C.
PspOMI  (9242)
1 site		 G G G C C C C C C G G G
XbaI  (9236)
1 site		 T C T A G A A G A T C T
PaeR7I  (9224)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (9224)
1 site		 V C T C G A G B B G A G C T C V
XhoI  (9224)
1 site		 C T C G A G G A G C T C
PstI  (9200)
1 site		 C T G C A G G A C G T C
EcoRI  (9191)
1 site		 G A A T T C C T T A A G
BamHI  (9160)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
SacI  (9158)
1 site		 G A G C T C C T C G A G
Eco53kI  (9156)
1 site		 G A G C T C C T C G A G
KpnI  (9152)
1 site		 G G T A C C C C A T G G
Acc65I  (9148)
1 site		 G G T A C C C C A T G G
HindIII  (9142)
1 site		 A A G