pCAMBIA1105.1R

Agrobacterium binary vector for plant transformation, with hygromycin- and spectinomycin-resistance and GUSPlus™ genes.

Sequence Author: Cambia

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NcoI (0) lacZα ApaI (11,431) PspOMI (11,427) XbaI (11,421) PstI (11,385) EcoRI (11,376) BamHI (11,345) SacI (11,343) Eco53kI (11,341) KpnI (11,337) Acc65I (11,333) HindIII (11,327) lac operator CaMV 35S promoter (enhanced) SmaI (10,279) TspMI - XmaI (10,277) AsiSI (9901) SacII (8682) BlpI (8051) HpaI (7524) BstZ17I (6451) BglII (7) SspI (137) BstBI (556) BsrGI (652) StuI (730) SnaBI (769) Bpu10I (915) AleI (1554) MluI (1696) BspHI * (1729) AfeI (1989) 6xHis PmlI (2048) AflII (2109) MauBI (2304) RB T-DNA repeat PasI (3797) AclI (4391) EcoNI (5780) pCAMBIA1105.1R 12,218 bp
NcoI  (0)
1 site
C C A T G G G G T A C C
ApaI  (11,431)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
PspOMI  (11,427)
1 site
G G G C C C C C C G G