pHELLSGATE

High-throughput binary Gateway® donor vector for silencing genes in plants using intron-containing hairpin RNA.
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Explore Over 2.7k Plasmids: Plant Vectors | More Plasmid Sets
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HomePlasmidsPlant VectorspHELLSGATE
AgeI (18,257) XbaI (17,913) XbaI (16,296) HindIII (16,284) BspDI - ClaI (16,279) PacI (16,168) KpnI (14,653) Acc65I (14,649) AbsI - XhoI (14,637) XhoI (13,020) StuI (12,234) SacI (11,646) Eco53kI (11,644) PmeI (11,220) BsiWI (11,055) FseI (10,595) Bsu36I (330) RsrII (1119) AflII (1923) MauBI (2118) LB T-DNA repeat AleI (5445) SexAI * (5533) FspAI (6101) NruI (6598) pHELLSGATE 18,691 bp
AgeI  (18,257)
1 site		 A C C G G T T G G C C A
XbaI  (17,913)
2 sites		 T C T A G A A G A T C T
XbaI  (16,296)
2 sites		 T C T A G A A G A T C T
HindIII  (16,284)
1 site		 A A G C T T T T C G A A
BspDI  (16,279)
1 site		 A T C G A T T A G C T A
ClaI  (16,279)
1 site		 A T C G A T T A G C T A
PacI  (16,168)
1 site		 T T A A T T A A A A T T A A T T
KpnI  (14,653)
1 site		 G G T A C C C C A T G G
Acc65I  (14,649)
1 site		 G G T A C C C C A T G G
AbsI  (14,637)
1 site		 C C T C G A G G G G A G C T C C
XhoI  (14,637)
2 sites		 C T C G A G G A G C T C
XhoI  (13,020)
2 sites		 C T C G A G G A G C T C
StuI  (12,234)
1 site		 A G G C C T T C C G G A
SacI  (11,646)
1 site		 G A G C T C C T C G A G
Eco53kI  (11,644)
1 site		 G A G C T C C T C G A G
PmeI  (11,220)
1 site		 G T T T A A A C C A A A T T T G
BsiWI  (11,055)
1 site		 C G T A C G G C A T G C
BsiWI is typically used at 55°C, but is 50% active at 37°C.
FseI  (10,595)
1 site		 G G C C G G C C C C G G C C G G
FseI gradually loses activity when stored at -20°C.
Bsu36I  (330)
1 site		 C C T N A G G G G A N T C C
Sticky ends from different Bsu36I sites may not be compatible.
RsrII  (1119)
1 site		 C G G W C C G G C C W G G C
Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
AflII  (1923)
1 site		 C T T A A G G A A T T C
MauBI  (2118)
1 site		 C G C G C G C G G C G C G C G C
AleI  (5445)
1 site		 C A C N N N N G T G G T G N N N N C A C
SexAI  (5533)
1 site		 A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
FspAI  (6101)
1 site		 R T G C G C A Y Y A C G C G