pEarleyGate 302
Gateway®-compatible promoterless plant transformation vector with a C-terminal FLAG tag.
Sequence Author: Pikaard Lab
Explore Over 2.7k Plasmids: Plant Vectors | More Plasmid Sets
No matches
|
| ||
| * Blocked by Dam methylation. BclI is typically used at 50-55°C, but is 50% active at 37°C. |
|
|
| ||
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage. |
|
| ||
Efficient cleavage requires at least two copies of the PluTI recognition sequence. |
|
| ||
Efficient cleavage requires at least two copies of the NarI recognition sequence. |
|
| ||
The 1-base overhangs produced by EcoNI may be hard to ligate.Sticky ends from different EcoNI sites may not be compatible. |
| ||
Sticky ends from different BseRI sites may not be compatible.BseRI quickly loses activity at 37°C.Prolonged incubation with BseRI may lead to degradation of the DNA. |
|
|
| ||
BsiWI is typically used at 55°C, but is 50% active at 37°C. |
|
|
|
|
| ||
Efficient cleavage requires at least two copies of the PaqCI recognition sequence. Sticky ends from different PaqCI sites may not be compatible.Cleavage can be improved with PaqCI Activator. |
|
|
|
|
|
|