pCAMBIA1305.1

Agrobacterium binary vector for plant transformation, with hygromycin- and kanamycin-resistance and GUSPlus™ genes.

Sequence Author: Cambia

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HomePlasmidsPlant VectorspCAMBIA1305.1
NcoI (0) lacZα HindIII (11,085) PstI - SbfI (11,077) SalI (11,067) XbaI (11,061) BamHI (11,055) KpnI (11,050) Acc65I (11,046) BanII - SacI (11,044) Eco53kI (11,042) EcoRI (11,034) lac operator lac promoter BstXI (10,791) AsiSI (9611) PspXI (8910) SacII (8392) PsiI (8264) BlpI (7788) BstZ17I (6451) MreI - SgrAI (6249) BglII (7) SpeI (216) BstBI (556) BsrGI (652) StuI (730) SnaBI (769) AleI (1554) MluI (1696) AfeI (1989) 6xHis PmlI (2048) BstEII (2058) AflII (2109) MauBI (2304) RB T-DNA repeat PasI (3797) AclI (4391) EcoNI (5780) pCAMBIA1305.1 11,847 bp
NcoI  (0)
1 site		 C C A T G G G G T A C C
HindIII  (11,085)
1 site		 A A G C T T T T C G A A
PstI  (11,077)
1 site		 C T G C A G G A C G T C
SbfI  (11,077)
1 site		 C C T G C A G G G G A C G T C C
SalI  (11,067)
1 site		 G T C G A C C A G C T G
XbaI  (11,061)
1 site		 T C T A G A A G A T C T
BamHI  (11,055)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
KpnI  (11,050)
1 site		 G G T A C C C C A T G G
Acc65I  (11,046)
1 site		 G G T A C C C C A T G G
BanII  (11,044)
1 site		 G R G C Y C C Y C G R G
Sticky ends from different BanII sites may not be compatible.
SacI  (11,044)
1 site		 G A G C T C C T C G A G
Eco53kI  (11,042)
1 site		 G A G C