pCAMBIA1305.1

Agrobacterium binary vector for plant transformation, with hygromycin- and kanamycin-resistance and GUSPlus™ genes.

Sequence Author: Cambia

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HomePlasmidsPlant VectorspCAMBIA1305.1
NcoI (0) lacZα HindIII (11,085) PstI - SbfI (11,077) SalI (11,067) XbaI (11,061) BamHI (11,055) KpnI (11,050) Acc65I (11,046) BanII - SacI (11,044) Eco53kI (11,042) EcoRI (11,034) lac operator lac promoter BstXI (10,791) AsiSI (9611) PspXI (8910) SacII (8392) PsiI (8264) BlpI (7788) BstZ17I (6451) MreI - SgrAI (6249) BglII (7) SpeI (216) BstBI (556) BsrGI (652) StuI (730) SnaBI (769) AleI (1554) MluI (1696) AfeI (1989) 6xHis PmlI (2048) BstEII (2058) AflII (2109) MauBI (2304) RB T-DNA repeat PasI (3797) AclI (4391) EcoNI (5780) pCAMBIA1305.1 11,847 bp
NcoI  (0)
1 site		 C C A T G G G G T A C C
HindIII  (11,085)
1 site		 A A G C T T T T C G A A
PstI  (11,077)
1 site		 C T G C A G G A C G T C
SbfI  (11,077)
1 site		 C C T G C A G G G G A C G T C C
SalI  (11,067)
1 site		 G T C G A C C A G C T G
XbaI  (11,061)
1 site		 T C T A G A A G A T C T
BamHI  (11,055)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
KpnI  (11,050)
1 site		 G G T A C C C C A T G G
Acc65I  (11,046)
1 site		 G G T A C C C C A T G G
BanII  (11,044)
1 site		 G R G C Y C C Y C G R G
Sticky ends from different BanII sites may not be compatible.
SacI  (11,044)
1 site		 G A G C T C C T C G A G
Eco53kI  (11,042)
1 site		 G A G C T C C T C G A G
EcoRI  (11,034)
1 site		 G A A T T C C T T A A G
BstXI  (10,791)
1 site		 C C A N N N N N N T G G G G T N N N N N N A C C
Sticky ends from different BstXI sites may not be compatible.
AsiSI  (9611)
1 site		 G C G A T C G C C G C T A G C G
PspXI  (8910)
1 site		 V C T C G A G B B G A G C T C V
SacII  (8392)
1 site		 C C G C G G G G C G C C
Efficient cleavage requires at least two copies of the SacII recognition sequence.
PsiI  (8264)
1 site		 T T A T A A A A T A T T
BlpI  (7788)
1 site		 G C T N A G C C G A N T C G
Sticky ends from different BlpI sites may not be compatible.
BstZ17I  (6451)
1 site		 G T A T A C C A T A T G
MreI  (6249)
1 site		 C G C C G G C G G C G G C C G C
SgrAI  (6249)
1 site		 C R C C G G Y G G Y G G C C R C
Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
BglII  (7)
1 site		 A G A T C T T C T A G A
SpeI  (216)
1 site		 A C T A G T T G A T C A
BstBI  (556)
1 site		 T T C G A A A A G C T T
BsrGI  (652)
1 site		 T G T A C A A C A T G T
BsrGI is typically used at 37°C, but is even more active at 60°C.
StuI  (730)
1 site		 A G G C C T T C C G G A
SnaBI  (769)
1 site		 T A C G T A A T G C A T
AleI  (1554)
1 site		 C A C N N N N G T G G T G N N N N C A C
MluI  (1696)
1 site		 A C G C G T T G C G C A
AfeI  (1989)
1 site		 A G C G C T T C G C G A
PmlI  (2048)
1 site		 C A C G T G G T G C A C
BstEII  (2058)
1 site		 G G T N A C C C C A N T G G
Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
AflII  (2109)
1 site		 C T T A A G G A A T T C
MauBI  (2304)
1 site		 C G C G C G C G G C G C G C G C
PasI  (3797)
1 site		 C C C W G G G G G G W C C C
Sticky ends from different PasI sites may not be compatible.
AclI  (4391)
1 site		 A A C G T T T T G C A A
EcoNI  (5780)
1 site		 C C T N N N N N A G G G G A N N N N N T C C
The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
GUSPlus™
2 .. 2024  =  2023 bp
611 amino acids  =  69.8 kDa
2 segments  =  1833 bp
Product: β-glucuronidase
codon-optimized Staphylococcus gusA gene with a catalase intron to ensure expression in plants but not bacteria
GUSPlus™
2 .. 2024  =  2023 bp
611 amino acids  =  69.8 kDa
2 segments  =  1833 bp
   Segment 1:  
   2 .. 16  =  15 bp
   5 amino acids  =  632.8 Da
Product: β-glucuronidase
codon-optimized Staphylococcus gusA gene with a catalase intron to ensure expression in plants but not bacteria
GUSPlus™
2 .. 2024  =  2023 bp
611 amino acids  =  69.8 kDa
2 segments  =  1833 bp
   Segment 2:  
   207 .. 2024  =  1818 bp
   606 amino acids  =  69.2 kDa
Product: β-glucuronidase
codon-optimized Staphylococcus gusA gene with a catalase intron to ensure expression in plants but not bacteria
GUSPlus™
2 .. 2024  =  2023 bp
611 amino acids  =  69.8 kDa
2 segments  =  1833 bp
Product: β-glucuronidase
codon-optimized Staphylococcus gusA gene with a catalase intron to ensure expression in plants but not bacteria
6xHis
2031 .. 2048  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
6xHis
2031 .. 2048  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
pVS1 RepA
4736 .. 5809  =  1074 bp
357 amino acids  =  39.9 kDa
Product: replication protein from Pseudomonas plasmid pVS1
pVS1 RepA
4736 .. 5809  =  1074 bp
357 amino acids  =  39.9 kDa
Product: replication protein from Pseudomonas plasmid pVS1
HygR
8949 .. 9974  =  1026 bp
341 amino acids  =  38.0 kDa
Product: hygromycin B phosphotransferase
confers resistance to hygromycin
HygR
8949 .. 9974  =  1026 bp
341 amino acids  =  38.0 kDa
Product: hygromycin B phosphotransferase
confers resistance to hygromycin
KanR
7414 .. 8208  =  795 bp
264 amino acids  =  31.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin
KanR
7414 .. 8208  =  795 bp
264 amino acids  =  31.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin
CaMV 35S promoter (enhanced)
10,042 .. 10,719  =  678 bp
cauliflower mosaic virus 35S promoter with a duplicated enhancer region
CaMV 35S promoter (enhanced)
10,042 .. 10,719  =  678 bp
cauliflower mosaic virus 35S promoter with a duplicated enhancer region
pVS1 StaA
3678 .. 4307  =  630 bp
209 amino acids  =  22.1 kDa
Product: stability protein from Pseudomonas plasmid pVS1
pVS1 StaA
3678 .. 4307  =  630 bp
209 amino acids  =  22.1 kDa
Product: stability protein from Pseudomonas plasmid pVS1
ori
6739 .. 7327  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
6739 .. 7327  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
CaMV 35S promoter
11,487 .. 11,832  =  346 bp
strong constitutive promoter from cauliflower mosaic virus
CaMV 35S promoter
11,487 .. 11,832  =  346 bp
strong constitutive promoter from cauliflower mosaic virus
NOS terminator
2080 .. 2332  =  253 bp
nopaline synthase terminator and poly(A) signal
NOS terminator
2080 .. 2332  =  253 bp
nopaline synthase terminator and poly(A) signal
lacZα
11,020 .. 11,253  =  234 bp
77 amino acids  =  8.6 kDa
Product: LacZα fragment of β-galactosidase
lacZα
11,020 .. 11,253  =  234 bp
77 amino acids  =  8.6 kDa
Product: LacZα fragment of β-galactosidase
pVS1 oriV
5875 .. 6069  =  195 bp
origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)
pVS1 oriV
5875 .. 6069  =  195 bp
origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)
CaMV poly(A) signal
8735 .. 8909  =  175 bp
cauliflower mosaic virus polyadenylation signal
CaMV poly(A) signal
8735 .. 8909  =  175 bp
cauliflower mosaic virus polyadenylation signal
bom
6413 .. 6553  =  141 bp
basis of mobility region from pBR322
bom
6413 .. 6553  =  141 bp
basis of mobility region from pBR322
lac promoter
10,946 .. 10,976  =  31 bp
3 segments
   Segment 1:  -35  
   10,946 .. 10,951  =  6 bp
promoter for the E. coli lac operon
lac promoter
10,946 .. 10,976  =  31 bp
3 segments
   Segment 2:  
   10,952 .. 10,969  =  18 bp
promoter for the E. coli lac operon
lac promoter
10,946 .. 10,976  =  31 bp
3 segments
   Segment 3:  -10  
   10,970 .. 10,976  =  7 bp
promoter for the E. coli lac operon
lac promoter
10,946 .. 10,976  =  31 bp
3 segments
promoter for the E. coli lac operon
RB T-DNA repeat
2354 .. 2378  =  25 bp
right border repeat from nopaline C58 T-DNA
RB T-DNA repeat
2354 .. 2378  =  25 bp
right border repeat from nopaline C58 T-DNA
LB T-DNA repeat
8633 .. 8657  =  25 bp
left border repeat from nopaline C58 T-DNA
LB T-DNA repeat
8633 .. 8657  =  25 bp
left border repeat from nopaline C58 T-DNA
lac operator
10,984 .. 11,000  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
10,984 .. 11,000  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
cat1 intron
17 .. 206  =  190 bp
castor bean catalase intron, modified
cat1 intron
17 .. 206  =  190 bp
castor bean catalase intron, modified
MCS
11,034 .. 11,090  =  57 bp
pUC18/19 multiple cloning site
MCS
11,034 .. 11,090  =  57 bp
pUC18/19 multiple cloning site
M13 rev
11,008 .. 11,024  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
11,008 .. 11,024  =  17 bp
common sequencing primer, one of multiple similar variants
M13 fwd
11,094 .. 11,110  =  17 bp
common sequencing primer, one of multiple similar variants
M13 fwd
11,094 .. 11,110  =  17 bp
common sequencing primer, one of multiple similar variants
ORF:  11,020 .. 11,253  =  234 bp
ORF:  77 amino acids  =  8.6 kDa
ORF:  11,305 .. 11,733  =  429 bp
ORF:  142 amino acids  =  15.8 kDa
ORF:  2693 .. 3379  =  687 bp
ORF:  228 amino acids  =  25.1 kDa
ORF:  4634 .. 5809  =  1176 bp
ORF:  391 amino acids  =  43.9 kDa
ORF:  7547 .. 7777  =  231 bp
ORF:  76 amino acids  =  8.3 kDa
ORF:  348 .. 2054  =  1707 bp
ORF:  568 amino acids  =  64.7 kDa
ORF:  3678 .. 4307  =  630 bp
ORF:  209 amino acids  =  22.1 kDa
ORF:  8901 .. 9641  =  741 bp
ORF:  246 amino acids  =  27.0 kDa
ORF:  2725 .. 3075  =  351 bp
ORF:  116 amino acids  =  12.6 kDa
ORF:  4582 .. 4839  =  258 bp
ORF:  85 amino acids  =  9.6 kDa
ORF:  7414 .. 8208  =  795 bp
ORF:  264 amino acids  =  31.0 kDa
ORF:  201 .. 2150  =  1950 bp
ORF:  649 amino acids  =  73.4 kDa
ORF:  3144 .. 3416  =  273 bp
ORF:  90 amino acids  =  9.9 kDa
ORF:  8949 .. 9974  =  1026 bp
ORF:  341 amino acids  =  38.0 kDa
ORF:  10,146 .. 10,463  =  318 bp
ORF:  105 amino acids  =  11.6 kDa
ORF:  10,473 .. 10,931  =  459 bp
ORF:  152 amino acids  =  16.9 kDa
ORF:  1388 .. 1615  =  228 bp
ORF:  75 amino acids  =  7.7 kDa
ORF:  3416 .. 3730  =  315 bp
ORF:  104 amino acids  =  12.0 kDa
ORF:  4604 .. 5176  =  573 bp
ORF:  190 amino acids  =  20.6 kDa
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Individual Sequences & Maps

pBI121
pCAMBIA1381Xa
pEarleyGate 201
pPZP100
pBI221
pCAMBIA1381Xb
pEarleyGate 202
pPZP101
pBin19
pCAMBIA1381Xc
pEarleyGate 203
pPZP102
pBINPLUS
pCAMBIA1381Z
pEarleyGate 204
pPZP111
pCAMBIA0105.1R
pCAMBIA1390
pEarleyGate 205
pPZP112
pCAMBIA0305.1
pCAMBIA1391
pEarleyGate 301
pPZP121
pCAMBIA0305.2
pCAMBIA1391Xa
pEarleyGate 302
pPZP122
pCAMBIA0380
pCAMBIA1391Xb
pEarleyGate 303
pPZP200
pCAMBIA0390
pCAMBIA1391Xc
pEarleyGate 304
pPZP201
pCAMBIA1105.1
pCAMBIA1391Z
pFGC5941
pPZP202
pCAMBIA1105.1R
pCAMBIA2200
pGA643
pPZP211
pCAMBIA1200
pCAMBIA2201
pGreen
pPZP212
pCAMBIA1201
pCAMBIA2300
pGreen 0029
pPZP221
pCAMBIA1281Z
pCAMBIA2301
pGreenII
pPZP222
pCAMBIA1291Z
pCAMBIA3200
pGreenII 0049
pRI 101-AN
pCAMBIA1300
pCAMBIA3201
pGreenII 0179
pRI 101-ON
pCAMBIA1301
pCAMBIA3300
pGreenII 0229
pRI 201-AN
pCAMBIA1302
pCAMBIA3301
pGreenII 0579
pRI 201-ON
pCAMBIA1303
pCAMBIA5105
pHANNIBAL
pRI 909
pCAMBIA1304
pEarleyGate 100
pHELLSGATE
pRI 910
pCAMBIA1305.1
pEarleyGate 101
pHELLSGATE 12
pSB1
pCAMBIA1305.2
pEarleyGate 102
pHELLSGATE 4
pSB11
pCAMBIA1380
pEarleyGate 103
pHELLSGATE 8
pSoup
pCAMBIA1381
pEarleyGate 104
pKANNIBAL

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