pEarleyGate 104

Gateway®-compatible plant transformation vector with a YFP N-terminal tag.

Sequence Author: Pikaard Lab

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BclI * (12,268) PsiI (12,137) BspHI (11,811) EcoRV (11,388) PluTI (10,129) SfoI (10,127) NarI (10,126) KasI (10,125) EcoNI (9653) BmtI (9344) NheI (9340) BsiWI (9224) PmeI (6212) AseI (92) FspAI (521) PaqCI (585) AfeI (758) StuI (1868) PshAI (2467) PspXI (2654) BbvCI (4691) SrfI (4837) BstXI (4954) XbaI (5235) PacI (5248) SpeI (5260) MfeI (5564) BstEII (5836) AhdI (5966) SbfI (5991) PvuI (6125) pEarleyGate 104 12,505 bp
BclI  (12,268)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
PsiI  (12,137)
1 site
T T A T A A A A T A T T
BspHI  (11,811)
1 site
T C A T G A A G T A C T
EcoRV  (11,388)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
PluTI  (10,129)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (10,127)
1 site
G G C G C C C C G C G G
NarI  (10,126)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (10,125)
1 site
G G C G C C C C G C G G
EcoNI  (9653)
1 site
C C T N N N N N A G G G G A N</