pPZP222
Agrobacterium binary vector for plant transformation, with spectinomycin- and gentamycin-resistance genes. The MCS is reversed in pPZP221.
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Sticky ends from different BanII sites may not be compatible. |
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SmaI can be used at 37°C for brief incubations. |
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Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present. |
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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Sticky ends from different BstXI sites may not be compatible. |
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Sticky ends from different BstEII sites may not be compatible.BstEII is typically used at 60°C, but is 50% active at 37°C. |
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Efficient cleavage requires at least two copies of the BpmI recognition sequence. Sticky ends from different BpmI sites may not be compatible.After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.BpmI quickly loses activity at 37°C. |
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Sticky ends from different BlpI sites may not be compatible. |
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BssHII is typically used at 50°C, but is 75% active at 37°C. |
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Sticky ends from different PasI sites may not be compatible. |
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BsiWI is typically used at 55°C, but is 50% active at 37°C. |
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| * Blocked by Dam methylation. |
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| * Blocked by Dam methylation. |
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The 1-base overhangs produced by EcoNI may be hard to ligate.Sticky ends from different EcoNI sites may not be compatible. |
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Sticky ends from different BsaI sites may not be compatible.BsaI can be used between 37°C and 50°C. |
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Efficient cleavage requires at least two copies of the NarI recognition sequence. |
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