pPZP222
Agrobacterium binary vector for plant transformation, with spectinomycin- and gentamycin-resistance genes. The MCS is reversed in pPZP221.
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Sticky ends from different BanII sites may not be compatible. |
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SmaI can be used at 37°C for brief incubations. |
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Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present. |
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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Sticky ends from different BstXI sites may not be compatible. |
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Sticky ends from different BstEII sites may not be compatible.BstEII is typically used at 60°C, but is 50% active at 37°C. |
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Efficient cleavage requires at least two copies of the BpmI recognition sequence. Sticky ends from different BpmI sites may not be compatible.After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.BpmI quickly loses activity at 37°C. |
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Sticky ends from different BlpI sites may not be compatible. |
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BssHII is typically used at 50°C, but is 75% active at 37°C. |
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Sticky ends from different PasI sites may not be compatible. |
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BsiWI is typically used at 55°C, but is 50% active at 37°C. |
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* Blocked by Dam methylation. |
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* Blocked by Dam methylation. |
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The 1-base overhangs produced by EcoNI may be hard to ligate.Sticky ends from different EcoNI sites may not be compatible. |
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Sticky ends from different BsaI sites may not be compatible.BsaI can be used between 37°C and 50°C. |
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Efficient cleavage requires at least two copies of the NarI recognition sequence. |
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Efficient cleavage requires at least two copies of the PluTI recognition sequence. |
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pVS1 RepA 2245 .. 3318 = 1074 bp 357 amino acids = 39.9 kDa Product: replication protein from Pseudomonas plasmid pVS1 |
pVS1 RepA 2245 .. 3318 = 1074 bp 357 amino acids = 39.9 kDa Product: replication protein from Pseudomonas plasmid pVS1 |
SmR 5080 .. 5871 = 792 bp 263 amino acids = 29.2 kDa Product: aminoglycoside adenylyltransferase confers resistance to spectinomycin and streptomycin |
SmR 5080 .. 5871 = 792 bp 263 amino acids = 29.2 kDa Product: aminoglycoside adenylyltransferase confers resistance to spectinomycin and streptomycin |
CaMV 35S promoter (enhanced) 7323 .. 8001 = 679 bp cauliflower mosaic virus 35S promoter with a duplicated enhancer region |
CaMV 35S promoter (enhanced) 7323 .. 8001 = 679 bp cauliflower mosaic virus 35S promoter with a duplicated enhancer region |
pVS1 StaA 1187 .. 1816 = 630 bp 209 amino acids = 22.1 kDa Product: stability protein from Pseudomonas plasmid pVS1 |
pVS1 StaA 1187 .. 1816 = 630 bp 209 amino acids = 22.1 kDa Product: stability protein from Pseudomonas plasmid pVS1 |
ori 4248 .. 4836 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
ori 4248 .. 4836 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
GmR 6730 .. 7263 = 534 bp 177 amino acids = 19.4 kDa Product: gentamycin acetyltransferase confers resistance to gentamycin |
GmR 6730 .. 7263 = 534 bp 177 amino acids = 19.4 kDa Product: gentamycin acetyltransferase confers resistance to gentamycin |
lacZα 8087 .. 8320 = 234 bp 77 amino acids = 8.6 kDa Product: LacZα fragment of β-galactosidase |
lacZα 8087 .. 8320 = 234 bp 77 amino acids = 8.6 kDa Product: LacZα fragment of β-galactosidase |
pVS1 oriV 3384 .. 3578 = 195 bp origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000) |
pVS1 oriV 3384 .. 3578 = 195 bp origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000) |
CaMV poly(A) signal 6521 .. 6695 = 175 bp cauliflower mosaic virus polyadenylation signal |
CaMV poly(A) signal 6521 .. 6695 = 175 bp cauliflower mosaic virus polyadenylation signal |
bom 3922 .. 4062 = 141 bp basis of mobility region from pBR322 |
bom 3922 .. 4062 = 141 bp basis of mobility region from pBR322 |
lac promoter 8364 .. 8394 = 31 bp 3 segments Segment 3: -10 8364 .. 8370 = 7 bp promoter for the E. coli lac operon |
lac promoter 8364 .. 8394 = 31 bp 3 segments Segment 2: 8371 .. 8388 = 18 bp promoter for the E. coli lac operon |
lac promoter 8364 .. 8394 = 31 bp 3 segments Segment 1: -35 8389 .. 8394 = 6 bp promoter for the E. coli lac operon |
lac promoter 8364 .. 8394 = 31 bp 3 segments promoter for the E. coli lac operon |
LB T-DNA repeat 6399 .. 6423 = 25 bp left border repeat from nopaline C58 T-DNA |
LB T-DNA repeat 6399 .. 6423 = 25 bp left border repeat from nopaline C58 T-DNA |
RB T-DNA repeat 8595 .. 8619 = 25 bp right border repeat from nopaline C58 T-DNA |
RB T-DNA repeat 8595 .. 8619 = 25 bp right border repeat from nopaline C58 T-DNA |
lac operator 8340 .. 8356 = 17 bp The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG). |
lac operator 8340 .. 8356 = 17 bp The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG). |
MCS 8250 .. 8306 = 57 bp pUC18 multiple cloning site |
MCS 8250 .. 8306 = 57 bp pUC18 multiple cloning site |
M13 fwd 8230 .. 8246 = 17 bp common sequencing primer, one of multiple similar variants |
M13 fwd 8230 .. 8246 = 17 bp common sequencing primer, one of multiple similar variants |
M13 rev 8316 .. 8332 = 17 bp common sequencing primer, one of multiple similar variants |
M13 rev 8316 .. 8332 = 17 bp common sequencing primer, one of multiple similar variants |
ORF: 202 .. 888 = 687 bp ORF: 228 amino acids = 25.1 kDa |
ORF: 2143 .. 3318 = 1176 bp ORF: 391 amino acids = 43.9 kDa |
ORF: 5449 .. 5832 = 384 bp ORF: 127 amino acids = 14.6 kDa |
ORF: 6136 .. 6477 = 342 bp ORF: 113 amino acids = 12.1 kDa |
ORF: 6868 .. 7200 = 333 bp ORF: 110 amino acids = 11.9 kDa |
ORF: 1187 .. 1816 = 630 bp ORF: 209 amino acids = 22.1 kDa |
ORF: 5294 .. 5572 = 279 bp ORF: 92 amino acids = 9.4 kDa |
ORF: 5582 .. 5839 = 258 bp ORF: 85 amino acids = 9.1 kDa |
ORF: 6024 .. 6266 = 243 bp ORF: 80 amino acids = 9.1 kDa |
ORF: 234 .. 584 = 351 bp ORF: 116 amino acids = 12.6 kDa |
ORF: 2091 .. 2348 = 258 bp ORF: 85 amino acids = 9.6 kDa |
ORF: 7428 .. 7745 = 318 bp ORF: 105 amino acids = 11.6 kDa |
ORF: 7755 .. 8093 = 339 bp ORF: 112 amino acids = 12.4 kDa |