pCAMBIA2200
Agrobacterium binary vector for plant transformation, with kanamycin- and chloramphenicol-resistance genes.
Sequence Author: Cambia
Explore Over 2.7k Plasmids: Plant Vectors | More Plasmid Sets
No matches
|
|
|
|
|
|
| ||
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
| ||
SmaI can be used at 37°C for brief incubations. |
|
|
| ||
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present. |
|
|
|
|
| ||
Sticky ends from different BstXI sites may not be compatible. |
| ||
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage. |
|
|
| ||
BssHII is typically used at 50°C, but is 75% active at 37°C. |
| ||
Efficient cleavage requires at least two copies of the RsrII recognition sequence. Sticky ends from different RsrII sites may not be compatible.For full activity, add fresh DTT. |
|
| ||
| * Blocked by Dam methylation. BclI is typically used at 50-55°C, but is 50% active at 37°C. |
|
|
|
|
| ||
| * Blocked by Dam methylation. |
| ||
BsiWI is typically used at 55°C, but is 50% active at 37°C. |
|
|
| ||
The 1-base overhangs produced by EcoNI may be hard to ligate.Sticky ends from different EcoNI sites may not be compatible. |
| ||
Sticky ends from different BsaI sites may not be compatible.BsaI can be used between 37°C and 50°C. |
|
|