After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
KpnI (11,565) 1 site
Acc65I (11,561) 1 site
BanII (11,559) 1 site
Sticky ends from different BanII sites may not be compatible.
SacI (11,559) 1 site
Eco53kI (11,557) 1 site
EcoRI (11,549) 1 site
BstXI (11,306) 1 site
Sticky ends from different BstXI sites may not be compatible.
AatII (10,465) 1 site
ZraI (10,463) 1 site
AsiSI (10,126) 1 site
RsrII (10,077) 1 site
Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.For full activity, add fresh DTT.
PspXI (9425) 1 site
SacII (8907) 1 site
Efficient cleavage requires at least two copies of the SacII recognition sequence.
PsiI (8779) 1 site
Bpu10I (8634) 1 site
Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.Sticky ends from different Bpu10I sites may not be compatible.
