pPZP112

Agrobacterium binary vector for plant transformation, with chloramphenicol- and kanamycin-resistance genes. The MCS is reversed in pPZP111.
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PmeI (8758) lac operator EcoRI (8479) SacI (8477) Eco53kI (8475) KpnI (8471) Acc65I (8467) SmaI (8465) TspMI - XmaI (8463) BamHI (8458) XbaI (8452) SalI (8446) PstI - SbfI (8444) HindIII (8428) BstXI (8251) EcoRV (7599) BglII (7453) NcoI (7438) BssHII (6908) RsrII (6790) PmlI (6522) BclI * (6056) BspEI (5552) SspI (5240) HpaI (5033) NsiI (4913) BsiWI (2860) NheI (2976) BmtI (2980) EcoNI (3289) BsaI (3379) AgeI (3470) BstZ17I (3960) pPZP112 8910 bp
PmeI  (8758)
1 site		 G T T T A A A C C A A A T T T G
EcoRI  (8479)
1 site		 G A A T T C C T T A A G
SacI  (8477)
1 site		 G A G C T C C T C G A G
Eco53kI  (8475)
1 site		 G A G C T C C T C G A G
KpnI  (8471)
1 site		 G G T A C C C C A T G G
Acc65I  (8467)
1 site		 G G T A C C C C A T G G
SmaI  (8465)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
TspMI  (8463)
1 site		 C C C G G G G G G C C C
XmaI  (8463)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
BamHI  (8458)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
XbaI  (8452)
1 site		 T C T A G A A G A T C T
SalI  (8446)
1 site		 G T C G A C C A G C T G
PstI  (8444)
1 site		 C T G C A G G A C G T C
SbfI  (8444)
1 site		 C C T G C A G G G G A C G T C C
HindIII  (8428)
1 site		 A A G C T T T T C G A A
BstXI  (8251)
1 site		 C C A N N N N N N T G G G G T N N N N N N A C C
Sticky ends from different BstXI sites may not be compatible.
EcoRV  (7599)
1 site		 G A T A T C C T A T A G
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
BglII  (7453)
1 site		 A G A T C T T C T A G A
NcoI  (7438)
1 site		 C C A T G G G G T A C C
BssHII  (6908)
1 site		 G C G C G C C G C G C G
BssHII is typically used at 50°C, but is 75% active at 37°C.
RsrII  (6790)
1 site		 C G G W C C G G C C W G G C
Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
PmlI  (6522)
1 site		 C A C G T G G T G C A C
BclI  (6056)
1 site		 T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BspEI  (5552)
1 site		 T C C G G A A G G C C T
SspI  (5240)
1 site		 A A T A T T T T A T A A
HpaI  (5033)
1 site		 G T T A A C C A A T T G
NsiI  (4913)
1 site		 A T G C A T T A C G T A
BsiWI  (2860)
1 site		 C G T A C G G C A T G C
BsiWI is typically used at 55°C, but is 50% active at 37°C.
NheI  (2976)
1 site		 G C T A G C C G A T C G
BmtI  (2980)
1 site		 G C T A G C C G A T C G
EcoNI  (3289)
1 site		 C C T N N N N N A G G G G A N N N N N T C C
The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
BsaI  (3379)
1 site		 G G T C T C N C C A G A G N ( N ) 4
Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AgeI  (3470)
1 site		 A C C G G T T G G C C A
BstZ17I  (3960)
1 site		 G T A T A C C A T A T G
pVS1 RepA
2245 .. 3318  =  1074 bp
357 amino acids  =  39.9 kDa
Product: replication protein from Pseudomonas plasmid pVS1
pVS1 RepA
2245 .. 3318  =  1074 bp
357 amino acids  =  39.9 kDa
Product: replication protein from Pseudomonas plasmid pVS1
NeoR/KanR
6644 .. 7441  =  798 bp
265 amino acids  =  29.1 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
NeoR/KanR
6644 .. 7441  =  798 bp
265 amino acids  =  29.1 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
CaMV 35S promoter (enhanced)
7501 .. 8179  =  679 bp
cauliflower mosaic virus 35S promoter with a duplicated enhancer region
CaMV 35S promoter (enhanced)
7501 .. 8179  =  679 bp
cauliflower mosaic virus 35S promoter with a duplicated enhancer region
CmR
5107 .. 5766  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
5107 .. 5766  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
pVS1 StaA
1187 .. 1816  =  630 bp
209 amino acids  =  22.1 kDa
Product: stability protein from Pseudomonas plasmid pVS1
pVS1 StaA
1187 .. 1816  =  630 bp
209 amino acids  =  22.1 kDa
Product: stability protein from Pseudomonas plasmid pVS1
ori
4248 .. 4836  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
4248 .. 4836  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
lacZα
8265 .. 8498  =  234 bp
77 amino acids  =  8.6 kDa
Product: LacZα fragment of β-galactosidase
lacZα
8265 .. 8498  =  234 bp
77 amino acids  =  8.6 kDa
Product: LacZα fragment of β-galactosidase
pVS1 oriV
3384 .. 3578  =  195 bp
origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)
pVS1 oriV
3384 .. 3578  =  195 bp
origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)
CaMV poly(A) signal
6416 .. 6590  =  175 bp
cauliflower mosaic virus polyadenylation signal
CaMV poly(A) signal
6416 .. 6590  =  175 bp
cauliflower mosaic virus polyadenylation signal
bom
3922 .. 4062  =  141 bp
basis of mobility region from pBR322
bom
3922 .. 4062  =  141 bp
basis of mobility region from pBR322
lac promoter
8542 .. 8572  =  31 bp
3 segments
   Segment 3:  -10  
   8542 .. 8548  =  7 bp
promoter for the E. coli lac operon
lac promoter
8542 .. 8572  =  31 bp
3 segments
   Segment 2:  
   8549 .. 8566  =  18 bp
promoter for the E. coli lac operon
lac promoter
8542 .. 8572  =  31 bp
3 segments
   Segment 1:  -35  
   8567 .. 8572  =  6 bp
promoter for the E. coli lac operon
lac promoter
8542 .. 8572  =  31 bp
3 segments
promoter for the E. coli lac operon
LB T-DNA repeat
6294 .. 6318  =  25 bp
left border repeat from nopaline C58 T-DNA
LB T-DNA repeat
6294 .. 6318  =  25 bp
left border repeat from nopaline C58 T-DNA
RB T-DNA repeat
8773 .. 8797  =  25 bp
right border repeat from nopaline C58 T-DNA
RB T-DNA repeat
8773 .. 8797  =  25 bp
right border repeat from nopaline C58 T-DNA
lac operator
8518 .. 8534  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
8518 .. 8534  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
MCS
8428 .. 8484  =  57 bp
pUC18 multiple cloning site
MCS
8428 .. 8484  =  57 bp
pUC18 multiple cloning site
M13 fwd
8408 .. 8424  =  17 bp
common sequencing primer, one of multiple similar variants
M13 fwd
8408 .. 8424  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
8494 .. 8510  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
8494 .. 8510  =  17 bp
common sequencing primer, one of multiple similar variants
ORF:  202 .. 888  =  687 bp
ORF:  228 amino acids  =  25.1 kDa
ORF:  2143 .. 3318  =  1176 bp
ORF:  391 amino acids  =  43.9 kDa
ORF:  6031 .. 6372  =  342 bp
ORF:  113 amino acids  =  12.1 kDa
ORF:  1187 .. 1816  =  630 bp
ORF:  209 amino acids  =  22.1 kDa
ORF:  6875 .. 7129  =  255 bp
ORF:  84 amino acids  =  9.7 kDa
ORF:  5919 .. 6161  =  243 bp
ORF:  80 amino acids  =  9.1 kDa
ORF:  925 .. 1239  =  315 bp
ORF:  104 amino acids  =  12.0 kDa
ORF:  2113 .. 2685  =  573 bp
ORF:  190 amino acids  =  20.6 kDa
ORF:  5107 .. 5907  =  801 bp
ORF:  266 amino acids  =  31.0 kDa
ORF:  6880 .. 7266  =  387 bp
ORF:  128 amino acids  =  14.6 kDa
ORF:  7606 .. 7923  =  318 bp
ORF:  105 amino acids  =  11.6 kDa
ORF:  7933 .. 8271  =  339 bp
ORF:  112 amino acids  =  12.4 kDa
ORF:  234 .. 584  =  351 bp
ORF:  116 amino acids  =  12.6 kDa
ORF:  2091 .. 2348  =  258 bp
ORF:  85 amino acids  =  9.6 kDa
ORF:  8265 .. 8498  =  234 bp
ORF:  77 amino acids  =  8.6 kDa
ORF:  653 .. 925  =  273 bp
ORF:  90 amino acids  =  9.9 kDa
ORF:  6644 .. 7441  =  798 bp
ORF:  265 amino acids  =  29.1 kDa
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Individual Sequences & Maps

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pCAMBIA0105.1R
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pPZP112
pCAMBIA0305.1
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pEarleyGate 301
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pCAMBIA1391Xa
pEarleyGate 302
pPZP122
pCAMBIA0380
pCAMBIA1391Xb
pEarleyGate 303
pPZP200
pCAMBIA0390
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pEarleyGate 304
pPZP201
pCAMBIA1105.1
pCAMBIA1391Z
pFGC5941
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pCAMBIA1105.1R
pCAMBIA2200
pGA643
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pCAMBIA1200
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pGreen
pPZP212
pCAMBIA1201
pCAMBIA2300
pGreen 0029
pPZP221
pCAMBIA1281Z
pCAMBIA2301
pGreenII
pPZP222
pCAMBIA1291Z
pCAMBIA3200
pGreenII 0049
pRI 101-AN
pCAMBIA1300
pCAMBIA3201
pGreenII 0179
pRI 101-ON
pCAMBIA1301
pCAMBIA3300
pGreenII 0229
pRI 201-AN
pCAMBIA1302
pCAMBIA3301
pGreenII 0579
pRI 201-ON
pCAMBIA1303
pCAMBIA5105
pHANNIBAL
pRI 909
pCAMBIA1304
pEarleyGate 100
pHELLSGATE
pRI 910
pCAMBIA1305.1
pEarleyGate 101
pHELLSGATE 12
pSB1
pCAMBIA1305.2
pEarleyGate 102
pHELLSGATE 4
pSB11
pCAMBIA1380
pEarleyGate 103
pHELLSGATE 8
pSoup
pCAMBIA1381
pEarleyGate 104
pKANNIBAL

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