pEarleyGate 202
Gateway®-compatible plant transformation vector with an N-terminal FLAG tag.
Sequence Author: Pikaard Lab
Explore Over 2.7k Plasmids: Plant Vectors | More Plasmid Sets
No matches
| ||
| * Blocked by Dam methylation. BclI is typically used at 50-55°C, but is 50% active at 37°C. |
|
|
| ||
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage. |
| ||
Efficient cleavage requires at least two copies of the PluTI recognition sequence. |
|
| ||
Efficient cleavage requires at least two copies of the NarI recognition sequence. |
|
| ||
The 1-base overhangs produced by EcoNI may be hard to ligate.Sticky ends from different EcoNI sites may not be compatible. |
|
|
| ||
BsiWI is typically used at 55°C, but is 50% active at 37°C. |
|
|
|
|
|
| ||
Efficient cleavage requires at least two copies of the PaqCI recognition sequence. Sticky ends from different PaqCI sites may not be compatible.Cleavage can be improved with PaqCI Activator. |
|
|
|
| ||
PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations. |
| ||
PaeR7I does not recognize the sequence CTCTCGAG. |
|
|
|
|
|
| ||
Sticky ends from different BstXI sites may not be compatible. |
|
|
|
|
| ||
Sticky ends from different BstEII sites may not be compatible.BstEII is typically used at 60°C, but is 50% active at 37°C. |
| ||
The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible. |
|
|
|
| pVS1 RepA 7880 .. 8953 = 1074 bp 357 amino acids = 39.9 kDa Product: replication protein from Pseudomonas plasmid pVS1 |
| pVS1 RepA 7880 .. 8953 = 1074 bp 357 amino acids = 39.9 kDa Product: replication protein from Pseudomonas plasmid pVS1 |
| KanR 10,558 .. 11,352 = 795 bp 264 amino acids = 31.0 kDa Product: aminoglycoside phosphotransferase confers resistance to kanamycin |
| KanR 10,558 .. 11,352 = 795 bp 264 amino acids = 31.0 kDa Product: aminoglycoside phosphotransferase confers resistance to kanamycin |
| OCS terminator 4547 .. 5254 = 708 bp octopine synthase terminator |
| OCS terminator 4547 .. 5254 = 708 bp octopine synthase terminator |
| CmR 3000 .. 3659 = 660 bp 219 amino acids = 25.7 kDa Product: chloramphenicol acetyltransferase confers resistance to chloramphenicol |
| CmR 3000 .. 3659 = 660 bp 219 amino acids = 25.7 kDa Product: chloramphenicol acetyltransferase confers resistance to chloramphenicol |
| pVS1 StaA 6822 .. 7451 = 630 bp 209 amino acids = 22.1 kDa Product: stability protein from Pseudomonas plasmid pVS1 |
| pVS1 StaA 6822 .. 7451 = 630 bp 209 amino acids = 22.1 kDa Product: stability protein from Pseudomonas plasmid pVS1 |
| ori 9883 .. 10,471 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
| ori 9883 .. 10,471 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
| BlpR 373 .. 924 = 552 bp 183 amino acids = 20.6 kDa Product: phosphinothricin acetyltransferase confers resistance to bialophos or phosphinothricin |
| BlpR 373 .. 924 = 552 bp 183 amino acids = 20.6 kDa Product: phosphinothricin acetyltransferase confers resistance to bialophos or phosphinothricin |
| MAS promoter 930 .. 1310 = 381 bp mannopine synthase promoter (Velten et al., 1984) |
| MAS promoter 930 .. 1310 = 381 bp mannopine synthase promoter (Velten et al., 1984) |
| CaMV 35S promoter 2306 .. 2651 = 346 bp strong constitutive promoter from cauliflower mosaic virus |
| CaMV 35S promoter 2306 .. 2651 = 346 bp strong constitutive promoter from cauliflower mosaic virus |
| ccdB 4001 .. 4306 = 306 bp 101 amino acids = 11.7 kDa Product: CcdB, a bacterial toxin that poisons DNA gyrase Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains. |
| ccdB 4001 .. 4306 = 306 bp 101 amino acids = 11.7 kDa Product: CcdB, a bacterial toxin that poisons DNA gyrase Plasmids containing the ccdB gene canno |