pEarleyGate 205
Gateway®-compatible plant transformation vector with a C-terminal TAP tag.
Sequence Author: Pikaard Lab
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| * Blocked by Dam methylation. BclI is typically used at 50-55°C, but is 50% active at 37°C. |
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EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage. |
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The 1-base overhangs produced by EcoNI may be hard to ligate.Sticky ends from different EcoNI sites may not be compatible. |
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BsiWI is typically used at 55°C, but is 50% active at 37°C. |
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Efficient cleavage requires at least two copies of the PaqCI recognition sequence. Sticky ends from different PaqCI sites may not be compatible.Cleavage can be improved with PaqCI Activator. |
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PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations. |
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PaeR7I does not recognize the sequence CTCTCGAG. |
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Sticky ends from different BstXI sites may not be compatible. |
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Sticky ends from different BstEII sites may not be compatible.BstEII is typically used at 60°C, but is 50% active at 37°C. |
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The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible. |
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| pVS1 RepA 8292 .. 9365 = 1074 bp 357 amino acids = 39.9 kDa Product: replication protein from Pseudomonas plasmid pVS1 |
| pVS1 RepA 8292 .. 9365 = 1074 bp 357 amino acids = 39.9 kDa Product: replication protein from Pseudomonas plasmid pVS1 |
| KanR 10,970 .. 11,764 = 795 bp 264 amino acids = 31.0 kDa Product: aminoglycoside phosphotransferase confers resistance to kanamycin |
| KanR 10,970 .. 11,764 = 795 bp 264 amino acids = 31.0 kDa Product: aminoglycoside phosphotransferase confers resistance to kanamycin |
| OCS terminator 4959 .. 5666 = 708 bp octopine synthase terminator |
| OCS terminator 4959 .. 5666 = 708 bp octopine synthase terminator |
| CmR 2896 .. 3555 = 660 bp 219 amino acids = 25.7 kDa Product: chloramphenicol acetyltransferase confers resistance to chloramphenicol |
| CmR 2896 .. 3555 = 660 bp 219 amino acids = 25.7 kDa Product: chloramphenicol acetyltransferase confers resistance to chlorampheni |