pEarleyGate 205
Gateway®-compatible plant transformation vector with a C-terminal TAP tag.
Sequence Author: Pikaard Lab
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| * Blocked by Dam methylation. BclI is typically used at 50-55°C, but is 50% active at 37°C. |
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EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage. |
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The 1-base overhangs produced by EcoNI may be hard to ligate.Sticky ends from different EcoNI sites may not be compatible. |
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BsiWI is typically used at 55°C, but is 50% active at 37°C. |
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Efficient cleavage requires at least two copies of the PaqCI recognition sequence. Sticky ends from different PaqCI sites may not be compatible.Cleavage can be improved with PaqCI Activator. |
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PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations. |
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PaeR7I does not recognize the sequence CTCTCGAG. |
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Sticky ends from different BstXI sites may not be compatible. |
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Sticky ends from different BstEII sites may not be compatible.BstEII is typically used at 60°C, but is 50% active at 37°C. |
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The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible. |
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| pVS1 RepA 8292 .. 9365 = 1074 bp 357 amino acids = 39.9 kDa Product: replication protein from Pseudomonas plasmid pVS1 |
| pVS1 RepA 8292 .. 9365 = 1074 bp 357 amino acids = 39.9 kDa Product: replication protein from Pseudomonas plasmid pVS1 |
| KanR 10,970 .. 11,764 = 795 bp 264 amino acids = 31.0 kDa Product: aminoglycoside phosphotransferase confers resistance to kanamycin |
| KanR 10,970 .. 11,764 = 795 bp 264 amino acids = 31.0 kDa Product: aminoglycoside phosphotransferase confers resistance to kanamycin |
| OCS terminator 4959 .. 5666 = 708 bp octopine synthase terminator |
| OCS terminator 4959 .. 5666 = 708 bp octopine synthase terminator |
| CmR 2896 .. 3555 = 660 bp 219 amino acids = 25.7 kDa Product: chloramphenicol acetyltransferase confers resistance to chloramphenicol |
| CmR 2896 .. 3555 = 660 bp 219 amino acids = 25.7 kDa Product: chloramphenicol acetyltransferase confers resistance to chloramphenicol |
| pVS1 StaA 7234 .. 7863 = 630 bp 209 amino acids = 22.1 kDa Product: stability protein from Pseudomonas plasmid pVS1 |
| pVS1 StaA 7234 .. 7863 = 630 bp 209 amino acids = 22.1 kDa Product: stability protein from Pseudomonas plasmid pVS1 |
| ori 10,295 .. 10,883 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
| ori 10,295 .. 10,883 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
| BlpR 373 .. 924 = 552 bp 183 amino acids = 20.6 kDa Product: phosphinothricin acetyltransferase confers resistance to bialophos or phosphinothricin |
| BlpR 373 .. 924 = 552 bp 183 amino acids = 20.6 kDa Product: phosphinothricin acetyltransferase confers resistance to bialophos or phosphinothricin |
| CBP 4381 .. 4458 = 78 bp 26 amino acids = 3.0 kDa Product: calmodulin-binding peptide derived from skeletal muscle myosin light chain kinase; binds calmodulin with nanomolar affinity in the presence of calcium |
| CBP 4381 .. 4458 = 78 bp 26 amino acids = 3.0 kDa Product: calmodulin-binding peptide derived from skeletal muscle myosin light chain kinase; binds calmodulin with nanomolar affinity in the presence of calcium |
| TEV Site 4486 .. 4506 = 21 bp 7 amino acids = 869.9 Da Product: tobacco etch virus (TEV) recognition and cleavage site |
| TEV Site 4486 .. 4506 = 21 bp 7 amino acids = 869.9 Da Product: tobacco etch virus (TEV) recognition and cleavage site |
| ProtA 4546 .. 4719 = 174 bp 58 amino acids = 6.6 kDa Product: IgG-binding unit of Staphylococcus aureus protein A |
| ProtA 4546 .. 4719 = 174 bp 58 amino acids = 6.6 kDa Product: IgG-binding unit of Staphylococcus aureus protein A |
| ProtA 4720 .. 4893 = 174 bp 58 amino acids = 6.6 kDa Product: IgG-binding unit of Staphylococcus aureus protein A |
| ProtA 4720 .. 4893 = 174 bp 58 amino acids = 6.6 kDa Product: IgG-binding unit of Staphylococcus aureus protein A |
| MAS promoter 930 .. 1310 = 381 bp mannopine synthase promoter (Velten et al., 1984) |
| MAS promoter 930 .. 1310 = 381 bp mannopine synthase promoter (Velten et al., 1984) |
| CaMV 35S promoter 2306 .. 2651 = 346 bp strong constitutive promoter from cauliflower mosaic virus |
| CaMV 35S promoter 2306 .. 2651 = 346 bp strong constitutive promoter from cauliflower mosaic virus |
| ccdB 3897 .. 4202 = 306 bp 101 amino acids = 11.7 kDa Product: CcdB, a bacterial toxin that poisons DNA gyrase Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains. |
| ccdB 3897 .. 4202 = 306 bp 101 amino acids = 11.7 kDa Product: CcdB, a bacterial toxin that poisons DNA gyrase Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains. |
| MAS terminator 111 .. 363 = 253 bp mannopine synthase terminator |
| MAS terminator 111 .. 363 = 253 bp mannopine synthase terminator |
| pVS1 oriV 9431 .. 9625 = 195 bp origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000) |
| pVS1 oriV 9431 .. 9625 = 195 bp origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000) |
| bom 9969 .. 10,109 = 141 bp basis of mobility region from pBR322 |
| bom 9969 .. 10,109 = 141 bp basis of mobility region from pBR322 |
| attR2 4243 .. 4367 = 125 bp recombination site for the Gateway® LR reaction |
| attR2 4243 .. 4367 = 125 bp recombination site for the Gateway® LR reaction |
| attR1 2663 .. 2782 = 120 bp recombination site for the Gateway® LR reaction |
| attR1 2663 .. 2782 = 120 bp recombination site for the Gateway® LR reaction |
| lac UV5 promoter 2812 .. 2842 = 31 bp 3 segments Segment 1: -35 2812 .. 2817 = 6 bp E. coli lac promoter with an "up" mutation |
| lac UV5 promoter 2812 .. 2842 = 31 bp 3 segments Segment 2: 2818 .. 2835 = 18 bp E. coli lac promoter with an "up" mutation |
| lac UV5 promoter 2812 .. 2842 = 31 bp 3 segments Segment 3: -10 2836 .. 2842 = 7 bp E. coli lac promoter with an "up" mutation |
| lac UV5 promoter 2812 .. 2842 = 31 bp 3 segments E. coli lac promoter with an "up" mutation |
| LB T-DNA repeat 1 .. 25 = 25 bp left border repeat from nopaline C58 T-DNA |
| LB T-DNA repeat 1 .. 25 = 25 bp left border repeat from nopaline C58 T-DNA |
| RB T-DNA repeat 5910 .. 5934 = 25 bp right border repeat from nopaline C58 T-DNA |
| RB T-DNA repeat 5910 .. 5934 = 25 bp right border repeat from nopaline C58 T-DNA |
| ORF: 448 .. 957 = 510 bp ORF: 169 amino acids = 18.0 kDa |
| ORF: 2896 .. 3555 = 660 bp ORF: 219 amino acids = 25.7 kDa |
| ORF: 4375 .. 4926 = 552 bp ORF: 183 amino acids = 20.5 kDa |
| ORF: 7234 .. 7863 = 630 bp ORF: 209 amino acids = 22.1 kDa |
| ORF: 275 .. 607 = 333 bp ORF: 110 amino acids = 11.9 kDa |
| ORF: 1730 .. 2134 = 405 bp ORF: 134 amino acids = 15.5 kDa |
| ORF: 5546 .. 5836 = 291 bp ORF: 96 amino acids = 10.1 kDa |
| ORF: 1809 .. 2072 = 264 bp ORF: 87 amino acids = 10.4 kDa |
| ORF: 2124 .. 2552 = 429 bp ORF: 142 amino acids = 15.9 kDa |
| ORF: 3897 .. 4202 = 306 bp ORF: 101 amino acids = 11.7 kDa |
| ORF: 6249 .. 6935 = 687 bp ORF: 228 amino acids = 25.1 kDa |
| ORF: 8190 .. 9365 = 1176 bp ORF: 391 amino acids = 43.9 kDa |
| ORF: 11,103 .. 11,333 = 231 bp ORF: 76 amino acids = 8.3 kDa |
| ORF: 6972 .. 7286 = 315 bp ORF: 104 amino acids = 12.0 kDa |
| ORF: 8160 .. 8732 = 573 bp ORF: 190 amino acids = 20.6 kDa |
| ORF: 1757 .. 2125 = 369 bp ORF: 122 amino acids = 14.2 kDa |
| ORF: 6281 .. 6631 = 351 bp ORF: 116 amino acids = 12.6 kDa |
| ORF: 8138 .. 8395 = 258 bp ORF: 85 amino acids = 9.6 kDa |
| ORF: 10,970 .. 11,764 = 795 bp ORF: 264 amino acids = 31.0 kDa |
| ORF: 12,009 .. 321 = 501 bp ORF: 166 amino acids = 18.5 kDa |
| ORF: 373 .. 924 = 552 bp ORF: 183 amino acids = 20.6 kDa |
| ORF: 6700 .. 6972 = 273 bp ORF: 90 amino acids = 9.9 kDa |
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