pBINPLUS

Improved binary Agrobacterium vector for plant transformation.
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Explore Over 2.7k Plasmids: Plant Vectors | More Plasmid Sets
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EcoNI (10,768) PacI (9595) EcoRI (9585) SacI (9583) Eco53kI (9581) KpnI (9577) Acc65I (9573) SmaI (9571) TspMI - XmaI (9569) BamHI (9564) XbaI (9558) SalI (9552) SbfI (9550) HindIII (9534) AscI (9527) lac operator XmnI (9230) BspDI - ClaI (9108) MauBI (8817) AflII (8622) ApaI (8474) PspOMI (8470) BstBI (8219) BmtI (7290) NheI (7286) Bsu36I (7271) AhdI (6401) BbvCI (6343) NotI (92) PasI (645) PshAI (1412) MluI (1649) BstEII (1752) ApaLI (1852) BstXI (2686) SfiI (4318) PflMI * (4647) NdeI (4876) AleI (5002) MfeI (5272) FspAI (5658) NruI (6155) KflI (6253) pBINPLUS 12,396 bp
EcoNI  (10,768)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
PacI  (9595)
1 site
T T A A T T A A A A T T A A T T
EcoRI  (9585)
1 site
G A A T T C C T T A A G
SacI  (9583)
1 site
G A G C T C C T C G A G
Eco53kI  (9581)
1 site
G A G C T C C T C G A G
KpnI  (9577)
1 site
G G T A C C C C A T G G
Acc65I  (9573)
1 site
G G T A C C C C A T G G
SmaI  (9571)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
TspMI  (9569)
1 site
C C C G G G G G G C C C
XmaI  (9569)
1 site
C C C G G G G G G C C C

Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
BamHI  (9564)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
XbaI  (9558)
1 site
T C T A G A A G A T C T
SalI  (9552)
1 site
G T C G A C C A G C T G
SbfI  (9550)
1 site
C C T G C A G G G G A C G T C C
HindIII  (9534)
1 site
A A G C T T T T C G A A
AscI  (9527)
1 site
G G C G C G C C C C G C G C G G
XmnI  (9230)
1 site
G A A N N N N T T C C T T N N N N A A G
BspDI  (9108)
1 site
A T C G A T T A G C T A
ClaI  (9108)
1 site
A T C G A T T A G C T A
MauBI  (8817)
1 site
C G C G C G C G G C G C G C G C
AflII  (8622)
1 site
C T T A A G G A A T T C
ApaI  (8474)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
PspOMI  (8470)
1 site
G G G C C C C C C G G G
BstBI  (8219)
1 site
T T C G A A A A G C T T
BmtI  (7290)
1 site
G C T A G C C G A T C G
NheI  (7286)
1 site
G C T A G C C G A T C G
Bsu36I  (7271)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
AhdI  (6401)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BbvCI  (6343)
1 site
C C T C A G C G G A G T C G
NotI  (92)
1 site
G C G G C C G C C G C C G G C G
PasI  (645)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
PshAI  (1412)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
MluI  (1649)
1 site
A C G C G T T G C G C A
BstEII  (1752)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
ApaLI  (1852)
1 site
G T G C A C C A C G T G
BstXI  (2686)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
SfiI  (4318)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
PflMI  (4647)
1 site
C C A N N N N N T G G G G T N N N N N A C C
* Blocked by Dcm methylation.
Sticky ends from different PflMI sites may not be compatible.
NdeI  (4876)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
AleI  (5002)
1 site
C A C N N N N G T G G T G N N N N C A C
MfeI  (5272)
1 site
C A A T T G G T T A A C
FspAI  (5658)
1 site
R T G C G C A Y Y A C G C G T R
NruI  (6155)
1 site
T C G C G A A G C G C T
KflI  (6253)
1 site
G G G W C C C C C C W G G G

Sticky ends from different KflI sites may not be compatible.
trfA
3964 .. 5112  =  1149 bp
382 amino acids  =  43.8 kDa
Product: trans-acting replication protein that binds to and activates oriV
trfA
3964 .. 5112  =  1149 bp
382 amino acids  =  43.8 kDa
Product: trans-acting replication protein that binds to and activates oriV
KanR
2871 .. 3665  =  795 bp
264 amino acids  =  31.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin
KanR
2871 .. 3665  =  795 bp
264 amino acids  =  31.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin
NeoR/KanR
7409 .. 8203  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
NeoR/KanR
7409 .. 8203  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
oriV
12,379 .. 694  =  712 bp
incP origin of replication
oriV
12,379 .. 694  =  712 bp
incP origin of replication
TetR
10,408 .. 11,058  =  651 bp
216 amino acids  =  23.3 kDa
Product: tetracycline resistance regulatory protein
TetR
10,408 .. 11,058  =  651 bp
216 amino acids  =  23.3 kDa
Product: tetracycline resistance regulatory protein
traJ
11,881 .. 12,252  =  372 bp
123 amino acids  =  13.5 kDa
Product: oriT-recognizing protein
traJ
11,881 .. 12,252  =  372 bp
123 amino acids  =  13.5 kDa
Product: oriT-recognizing protein
pBR322 ori (truncated)
1758 .. 2110  =  353 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication (truncated)
pBR322 ori (truncated)
1758 .. 2110  =  353 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication (truncated)
lacZα
9504 .. 9854  =  351 bp
116 amino acids  =  13.1 kDa
Product: LacZα fragment of β-galactosidase
lacZα
9504 .. 9854  =  351 bp
116 amino acids  =  13.1 kDa
Product: LacZα fragment of β-galactosidase
NOS terminator
8593 .. 8845  =  253 bp
nopaline synthase terminator and poly(A) signal
NOS terminator
8593 .. 8845  =  253 bp
nopaline synthase terminator and poly(A) signal
NOS promoter
7173 .. 7352  =  180 bp
nopaline synthase promoter
NOS promoter
7173 .. 7352  =  180 bp
nopaline synthase promoter
oriT
11,739 .. 11,848  =  110 bp
incP origin of transfer
oriT
11,739 .. 11,848  =  110 bp
incP origin of transfer
lac promoter
9430 .. 9460  =  31 bp
3 segments
   Segment 1:  -35  
   9430 .. 9435  =  6 bp
promoter for the E. coli lac operon
lac promoter
9430 .. 9460  =  31 bp
3 segments
   Segment 2:  
   9436 .. 9453  =  18 bp
promoter for the E. coli lac operon
lac promoter
9430 .. 9460  =  31 bp
3 segments
   Segment 3:  -10  
   9454 .. 9460  =  7 bp
promoter for the E. coli lac operon
lac promoter
9430 .. 9460  =  31 bp
3 segments
promoter for the E. coli lac operon
LB T-DNA repeat
6708 .. 6732  =  25 bp
left border repeat from nopaline C58 T-DNA
LB T-DNA repeat
6708 .. 6732  =  25 bp
left border repeat from nopaline C58 T-DNA
RB T-DNA repeat
9919 .. 9943  =  25 bp
right border repeat from nopaline C58 T-DNA
RB T-DNA repeat
9919 .. 9943  =  25 bp
right border repeat from nopaline C58 T-DNA
lac operator
9468 .. 9484  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
9468 .. 9484  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
MCS
9526 .. 9598  =  73 bp
multiple cloning site
MCS
9526 .. 9598  =  73 bp
multiple cloning site
M13 rev
9492 .. 9508  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
9492 .. 9508  =  17 bp
common sequencing primer, one of multiple similar variants
M13 fwd
9605 .. 9621  =  17 bp
common sequencing primer, one of multiple similar variants
M13 fwd
9605 .. 9621  =  17 bp
common sequencing primer, one of multiple similar variants
ORF:  688 .. 993  =  306 bp
ORF:  101 amino acids  =  9.6 kDa
ORF:  1366 .. 1656  =  291 bp
ORF:  96 amino acids  =  11.0 kDa
ORF:  3673 .. 3915  =  243 bp
ORF:  80 amino acids  =  9.0 kDa
ORF:  3964 .. 5112  =  1149 bp
ORF:  382 amino acids  =  43.8 kDa
ORF:  6586 .. 6810  =  225 bp
ORF:  74 amino acids  =  8.2 kDa
ORF:  8224 .. 8616  =  393 bp
ORF:  130 amino acids  =  14.4 kDa
ORF:  10,021 .. 10,362  =  342 bp
ORF:  113 amino acids  =  13.0 kDa
ORF:  10,381 .. 11,058  =  678 bp
ORF:  225 amino acids  =  24.3 kDa
ORF:  11,881 .. 12,252  =  372 bp
ORF:  123 amino acids  =  13.5 kDa
ORF:  3686 .. 3967  =  282 bp
ORF:  93 amino acids  =  10.4 kDa
ORF:  4553 .. 4825  =  273 bp
ORF:  90 amino acids  =  10.2 kDa
ORF:  7409 .. 8203  =  795 bp
ORF:  264 amino acids  =  29.0 kDa
ORF:  8999 .. 9337  =  339 bp
ORF:  112 amino acids  =  11.7 kDa
ORF:  12,308 .. 139  =  228 bp
ORF:  75 amino acids  =  8.5 kDa
ORF:  2871 .. 3665  =  795 bp
ORF:  264 amino acids  =  31.0 kDa
ORF:  4692 .. 5087  =  396 bp
ORF:  131 amino acids  =  14.1 kDa
ORF:  5367 .. 6278  =  912 bp
ORF:  303 amino acids  =  32.1 kDa
ORF:  7581 .. 7967  =  387 bp
ORF:  128 amino acids  =  14.6 kDa
ORF:  9504 .. 9854  =  351 bp
ORF:  116 amino acids  =  13.1 kDa
ORF:  544 .. 984  =  441 bp
ORF:  146 amino acids  =  16.0 kDa
ORF:  2164 .. 2430  =  267 bp
ORF:  88 amino acids  =  10.1 kDa
ORF:  4705 .. 5100  =  396 bp
ORF:  131 amino acids  =  14.7 kDa
ORF:  9964 .. 10,377  =  414 bp
ORF:  137 amino acids  =  14.9 kDa
ORF:  417 .. 929  =  513 bp
ORF:  170 amino acids  =  17.9 kDa
ORF:  1332 .. 1709  =  378 bp
ORF:  125 amino acids  =  14.9 kDa
ORF:  3906 .. 4493  =  588 bp
ORF:  195 amino acids  =  20.7 kDa
ORF:  5262 .. 5528  =  267 bp
ORF:  88 amino acids  =  9.8 kDa
ORF:  8283 .. 8663  =  381 bp
ORF:  126 amino acids  =  13.7 kDa
ORF:  3302 .. 3532  =  231 bp
ORF:  76 amino acids  =  8.3 kDa
ORF:  7718 .. 8254  =  537 bp
ORF:  178 amino acids  =  19.8 kDa
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Download pBINPLUS.dna file

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