pAd CMV V5-DEST

Adenoviral Gateway® destination vector for constitutive expression of a C-terminally V5-tagged protein.

Sequence Author: Thermo Fisher (Invitrogen)

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5' ITR MreI (26,932) AsiSI (21,985) Ad5 Ψ CMV promoter T7 promoter attR1 V5 tag HSV TK poly(A) signal pAd/CMV/V5-DEST™ 36,686 bp
MreI  (26,932)
1 site
C G C C G G C G G C G G C C G C
AsiSI  (21,985)
1 site
G C G A T C G C C G C T A G C G
adenoviral DNA
4056 .. 34,604  =  30,549 bp
sequences from human adenovirus serotype 5, lacking the E1 and E3 regions
adenoviral DNA
4056 .. 34,604  =  30,549 bp
sequences from human adenovirus serotype 5, lacking the E1 and E3 regions
AmpR
35,568 .. 36,428  =  861 bp
286 amino acids  =  ~31.6 kDa
2 segments
   Segment 2:  
   35,568 .. 36,359  =  792 bp
   263 amino acids  =  ~29.0 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
35,568 .. 36,428  =  861 bp
286 amino acids  =  ~31.6 kDa
2 segments
   Segment 1:  signal sequence  
   36,360 .. 36,428  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
35,568 .. 36,428  =  861 bp
286 amino acids  =  ~31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
CmR
2607 .. 3266  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
2607 .. 3266  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
ori
34,828 .. 35,416  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
34,828 .. 35,416  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
CMV enhancer
731 .. 1110  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
731 .. 1110  =  380 bp
human cytomegalovirus immediate early enhancer
ccdB
1960 .. 2265  =  306 bp
101 amino acids  =  11.7 kDa
Product: CcdB, a bacterial toxin that poisons DNA gyrase
Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains.
ccdB
1960 .. 2265  =  306 bp
101 amino acids  =  11.7 kDa
Product: CcdB, a bacterial toxin that poisons DNA gyrase
Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains.
CMV promoter
1111 .. 1314  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
1111 .. 1314  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
Ad5 Ψ
191 .. 341  =  151 bp
packaging signal for adenovirus serotype 5
Ad5 Ψ
191 .. 341  =  151 bp
packaging signal for adenovirus serotype 5
attR1
1407 .. 1531  =  125 bp
recombination site for the Gateway® LR reaction
attR1
1407 .. 1531  =  125 bp
recombination site for the Gateway® LR reaction
attR2
3547 .. 3671  =  125 bp
recombination site for the Gateway® LR reaction
attR2
3547 .. 3671  =  125 bp
recombination site for the Gateway® LR reaction
AmpR promoter
36,429 .. 36,533  =  105 bp
AmpR promoter
36,429 .. 36,533  =  105 bp
5' ITR
1 .. 103  =  103 bp
inverted terminal repeat of human adenovirus serotype 5
5' ITR
1 .. 103  =  103 bp
inverted terminal repeat of human adenovirus serotype 5
cat promoter
3267 .. 3369  =  103 bp
promoter of the E. coli cat gene
cat promoter
3267 .. 3369  =  103 bp
promoter of the E. coli cat gene
HSV TK poly(A) signal
3822 .. 3870  =  49 bp
herpesvirus thymidine kinase polyadenylation signal
HSV TK poly(A) signal
3822 .. 3870  =  49 bp
herpesvirus thymidine kinase polyadenylation signal
V5 tag
3697 .. 3738  =  42 bp
14 amino acids  =  1.4 kDa
Product: epitope tag from simian virus 5
V5 tag
3697 .. 3738  =  42 bp
14 amino acids  =  1.4 kDa
Product: epitope tag from simian virus 5
T7 promoter
1359 .. 1377  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
1359 .. 1377  =  19 bp
promoter for bacteriophage T7 RNA polymerase
3' ITR
34,502 .. 34,604  =  103 bp
inverted terminal repeat of human adenovirus serotype 5
3' ITR
34,502 .. 34,604  =  103 bp
inverted terminal repeat of human adenovirus serotype 5
ORF:  8521 .. 8970  =  450 bp
ORF:  149 amino acids  =  16.7 kDa
ORF:  9847 .. 10,353  =  507 bp
ORF:  168 amino acids  =  17.7 kDa
ORF:  11,593 .. 12,840  =  1248 bp
ORF:  415 amino acids  =  47.1 kDa
ORF:  22,276 .. 22,890  =  615 bp
ORF:  204 amino acids  =  23.1 kDa
ORF:  24,022 .. 27,027  =  3006 bp
ORF:  1001 amino acids  =  110.0 kDa
ORF:  29,650 .. 31,452  =  1803 bp
ORF:  600 amino acids  =  63.6 kDa
ORF:  35,698 .. 35,964  =  267 bp
ORF:  88 amino acids  =  ~9.3 kDa
ORF:  6833 .. 7153  =  321 bp
ORF:  106 amino acids  =  11.7 kDa
ORF:  7853 .. 8104  =  252 bp
ORF:  83 amino acids  =  9.4 kDa
ORF:  9038 .. 9274  =  237 bp
ORF:  78 amino acids  =  7.9 kDa
ORF:  14,699 .. 16,414  =  1716 bp
ORF:  571 amino acids  =  63.3 kDa
ORF:  16,421 .. 17,017  =  597 bp
ORF:  198 amino acids  =  22.0 kDa
ORF:  17,054 .. 18,193  =  1140 bp
ORF:  379 amino acids  =  42.6 kDa
ORF:  18,221 .. 18,463  =  243 bp
ORF:  80 amino acids  =  8.8 kDa
ORF:  19,385 .. 22,243  =  2859 bp
ORF:  952 amino acids  =  108.0 kDa
ORF:  24,278 .. 24,634  =  357 bp
ORF:  118 amino acids  =  12.8 kDa
ORF:  24,977 .. 25,363  =  387 bp
ORF:  128 amino acids  =  14.3 kDa
ORF:  26,540 .. 27,328  =  789 bp
ORF:  262 amino acids  =  28.9 kDa
ORF:  31,400 .. 31,798  =  399 bp
ORF:  132 amino acids  =  15.0 kDa
ORF:  32,621 .. 33,118  =  498 bp
ORF:  165 amino acids  =  18.7 kDa
ORF:  34,673 .. 34,909  =  237 bp
ORF:  78 amino acids  =  8.9 kDa
ORF:  4152 .. 4574  =  423 bp
ORF:  140 amino acids  =  14.5 kDa
ORF:  10,974 .. 11,387  =  414 bp
ORF:  137 amino acids  =  14.4 kDa
ORF:  12,861 .. 14,618  =  1758 bp
ORF:  585 amino acids  =  65.3 kDa
ORF:  17,829 .. 18,308  =  480 bp
ORF:  159 amino acids  =  17.4 kDa
ORF:  18,546 .. 19,298  =  753 bp
ORF:  250 amino acids  =  27.0 kDa
ORF:  27,717 .. 28,400  =  684 bp
ORF:  227 amino acids  =  24.7 kDa
ORF:  28,401 .. 28,724  =  324 bp
ORF:  107 amino acids  =  12.3 kDa
ORF:  29,142 .. 29,504  =  363 bp
ORF:  120 amino acids  =  13.7 kDa
ORF:  33,132 .. 33,470  =  339 bp
ORF:  112 amino acids  =  12.5 kDa
ORF:  34,755 .. 35,153  =  399 bp
ORF:  132 amino acids  =  14.8 kDa
ORF:  2607 .. 3266  =  660 bp
ORF:  219 amino acids  =  25.7 kDa
ORF:  5880 .. 6227  =  348 bp
ORF:  115 amino acids  =  12.2 kDa
ORF:  9126 .. 11,087  =  1962 bp
ORF:  653 amino acids  =  74.7 kDa
ORF:  12,102 .. 12,395  =  294 bp
ORF:  97 amino acids  =  10.5 kDa
ORF:  19,008 .. 19,337  =  330 bp
ORF:  109 amino acids  =  11.4 kDa
ORF:  22,986 .. 24,575  =  1590 bp
ORF:  529 amino acids  =  59.1 kDa
ORF:  26,970 .. 27,260  =  291 bp
ORF:  96 amino acids  =  10.5 kDa
ORF:  29,823 .. 30,293  =  471 bp
ORF:  156 amino acids  =  16.6 kDa
ORF:  30,627 .. 30,872  =  246 bp
ORF:  81 amino acids  =  8.7 kDa
ORF:  33,018 .. 33,368  =  351 bp
ORF:  116 amino acids  =  13.3 kDa
ORF:  35,568 .. 36,428  =  861 bp
ORF:  286 amino acids  =  ~31.6 kDa
ORF:  3869 .. 4207  =  339 bp
ORF:  112 amino acids  =  12.2 kDa
ORF:  4634 .. 5992  =  1359 bp
ORF:  452 amino acids  =  51.2 kDa
ORF:  6995 .. 7333  =  339 bp
ORF:  112 amino acids  =  12.8 kDa
ORF:  9005 .. 9583  =  579 bp
ORF:  192 amino acids  =  20.8 kDa
ORF:  11,297 .. 11,662  =  366 bp
ORF:  121 amino acids  =  12.7 kDa
ORF:  12,857 .. 13,117  =  261 bp
ORF:  86 amino acids  =  9.0 kDa
ORF:  14,924 .. 15,709  =  786 bp
ORF:  261 amino acids  =  28.5 kDa
ORF:  17,186 .. 17,455  =  270 bp
ORF:  89 amino acids  =  9.5 kDa
ORF:  18,704 .. 19,252  =  549 bp
ORF:  182 amino acids  =  18.9 kDa
ORF:  32,663 .. 33,007  =  345 bp
ORF:  114 amino acids  =  13.3 kDa
ORF:  33,806 .. 34,192  =  387 bp
ORF:  128 amino acids  =  14.3 kDa
ORF:  34,562 .. 34,858  =  297 bp
ORF:  98 amino acids  =  10.8 kDa
ORF:  1960 .. 2265  =  306 bp
ORF:  101 amino acids  =  11.7 kDa
ORF:  5017 .. 5241  =  225 bp
ORF:  74 amino acids  =  8.3 kDa
ORF:  5740 .. 8910  =  3171 bp
ORF:  1056 amino acids  =  120.4 kDa
ORF:  11,803 .. 12,171  =  369 bp
ORF:  122 amino acids  =  13.6 kDa
ORF:  17,740 .. 17,991  =  252 bp
ORF:  83 amino acids  =  8.3 kDa
ORF:  18,190 .. 18,480  =  291 bp
ORF:  96 amino acids  =  10.9 kDa
ORF:  19,417 .. 19,692  =  276 bp
ORF:  91 amino acids  =  10.2 kDa
ORF:  31,858 .. 32,742  =  885 bp
ORF:  294 amino acids  =  34.1 kDa
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Download pAd CMV V5-DEST.dna file

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