pLVX-TetOne
All-in-one lentiviral vector for strong inducible expression of genes using the Tet-On® system.
Sequence Author: Clontech (TaKaRa)
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Sticky ends from different BglI sites may not be compatible. |
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Sticky ends from different DrdI sites may not be compatible. |
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PciI is inhibited by nonionic detergents. |
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Sticky ends from different Bsu36I sites may not be compatible. |
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Efficient cleavage requires at least two copies of the SacII recognition sequence. |
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Sticky ends from different PflMI sites may not be compatible. |
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SmaI can be used at 37°C for brief incubations. |
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Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present. |
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FseI gradually loses activity when stored at -20°C. |
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Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present. This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.Sticky ends from different Bpu10I sites may not be compatible. |
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Sticky ends from different BstAPI sites may not be compatible. |
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Sticky ends from different KflI sites may not be compatible. |
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