pLVX-TetOne

All-in-one lentiviral vector for strong inducible expression of genes using the Tet-On® system.

Sequence Author: Clontech (TaKaRa)

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HomePlasmidsViral Expression & Packaging VectorspLVX-TetOne
SgrDI (7911) SspI (7794) PvuI (7360) BglI (7110) DrdI (6208) PciI (6100) BmtI (5716) NheI (5712) KpnI (4930) Acc65I (4926) Bsu36I (4922) PstI (4806) SacII (4704) NcoI (4609) PflMI (4442) MluI (4188) SmaI (4181) TspMI - XmaI (4179) FseI (1150) BbvCI - Bpu10I (1424) BstAPI (1453) AleI (1577) KflI - PpuMI (1934) cPPT/CTS BamHI (2496) AgeI - SgrAI (2508) BstZ17I (2516) EcoRI (2522) PaeR7I - PspXI - XhoI (2893) BstXI (2915) BsmBI - Esp3I - SpeI (3168) MscI (3234) AscI (3257) XbaI (3444) BfuAI - BspMI (3998) NdeI (4020) pLVX-TetOne™ 8279 bp
SgrDI  (7911)
1 site		 C G T C G A C G G C A G C T G C
SspI  (7794)
1 site		 A A T A T T T T A T A A
PvuI  (7360)
1 site		 C G A T C G G C T A G C
BglI  (7110)
1 site		 G C C N N N N N G G C C G G N N N N N C C G
Sticky ends from different BglI sites may not be compatible.
DrdI  (6208)
1 site		 G A C N N N N N N G T C C T G N N N N N N C A G
Sticky ends from different DrdI sites may not be compatible.
PciI  (6100)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
BmtI  (5716)
1 site		 G C T A G C C G A T C G
NheI  (5712)
1 site		 G C T A G C C G A T C G
KpnI  (4930)
1 site		 G G T A C C C C A T G G
Acc65I  (4926)
1 site		 G G T A C C C C A T G G
Bsu36I  (4922)
1 site		 C C T N A G G G G A N T C C
Sticky ends from different Bsu36I sites may not be compatible.
PstI  (4806)
1 site		 C T G C A G G A C G T C
SacII  (4704)
1 site		 C C G C G G G G C G C C
Efficient cleavage requires at least two copies of the SacII recognition sequence.
NcoI  (4609)
1 site		 C C A T G G G G T A C C
PflMI  (4442)
1 site		 C C A N N N N N T G G G G T N N N N N A C C
Sticky ends from different PflMI sites may not be compatible.
MluI  (4188)
1 site		 A C G C G T T G C G C A
SmaI  (4181)
1 site		 C C C G G G G G G C C