pGIPZ

Empty lentiviral vector for constitutive expression of an shRNAmir together with TurboGFP.

Sequence Author: Open Biosystems

|Download SnapGene Viewer
No matches
AmpR promoter SspI (11,251) FspI (10,669) AhdI (10,447) BstZ17I (9175) AsiSI (8252) PshAI (7915) PmeI (6776) Bsu36I (6383) MluI (5650) SgrDI (11,368) NruI * (833) EcoNI (1170) BbvCI - Bpu10I (1424) AleI (1577) KflI - PpuMI (1934) SgrAI (2500) MauBI (2578) SfiI (2621) XbaI (2707) SnaBI (3070) CMV promoter BlpI (3564) BsrGI (4089) NotI (4100) BsiWI (4749) HpaI (5376) PaeR7I - PspXI - XhoI (5391) BamHI (5403) pGIPZ 11,688 bp
SspI  (11,251)
1 site
A A T A T T T T A T A A
FspI  (10,669)
1 site
T G C G C A A C G C G T
AhdI  (10,447)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BstZ17I  (9175)
1 site
G T A T A C C A T A T G
AsiSI  (8252)
1 site
G C G A T C G C C G C T A G C G
PshAI  (7915)
1 site
G A C N N N N