pShuttle2

Vector for creating a gene expression cassette that can be transferred to a replication-deficient Ad5 genome.

Sequence Author: Clontech (TaKaRa)

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HomePlasmidsViral Expression & Packaging VectorspShuttle2
Bsu36I (17) DraIII (3627) BsrDI (3603) BspDI - ClaI (3400) SmaI (3219) TspMI - XmaI (3217) EcoNI (3180) SspI (3168) BsrFI (3135) AsiSI - PvuI (3095) BsmBI - Esp3I (3073) PflMI (2833) BstEII (2163) I-CeuI (20) MfeI (184) MluI (251) HincII (257) SpeI (272) NdeI (507) BmrI (562) BsaAI - SnaBI (613) NcoI - StyI (633) CMV promoter BsaI (899) NheI (918) BmtI (922) DraI - PmeI (927) PspOMI (932) EcoO109I (933) ApaI (936) XbaI (938) EagI - NotI (950) BstXI (963) Acc65I (981) KpnI (985) AflII (990) stop codons PsiI (1026) PstI (1121) EcoRI (1127) PI-SceI (1147) BspQI - SapI (1248) PciI (1364) NspI (1368) DrdI (1472) BseYI (1668) PspFI (1672) AcuI - Eco57MI (1912) pShuttle2 3974 bp
Bsu36I  (17)
1 site		 C C T N A G G G G A N T C C
Sticky ends from different Bsu36I sites may not be compatible.
DraIII  (3627)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
BsrDI  (3603)
1 site		 G C A A T G N N C G T T A C
Sticky ends from different BsrDI sites may not be compatible.
BspDI  (3400)
1 site		 A T C G A T T A G C T A
ClaI  (3400)
1 site		 A T C G A T T A G C T A
SmaI  (3219)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
TspMI  (3217)
1 site		 C C C G G G G G G C C C
XmaI  (3217)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
EcoNI  (3180)
1 site		 C C T N N N N N A G G G G A N N N N N T C C
The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
SspI  (3168)
1 site		 A A T A T T T T A T A A
BsrFI  (3135)
1 site		 R C C G G Y Y G G C C R
Cleavage may be enhanced when more than one copy of the BsrFI recognition sequence is present.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
AsiSI  (3095)
1 site		 G C G A T C G C C G C T A G C G
PvuI  (3095)
1 site		 C G A T C G G C T A G C
BsmBI  (3073)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
Esp3I  (3073)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different Esp3I sites may not be compatible.
PflMI  (2833)
1 site		 C C A N N N N N T G G G G T N N N N N A C C
Sticky ends from different PflMI sites may not be compatible.
BstEII  (2163)
1 site		 G G T N A C C C C A N T G G
Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
I-CeuI  (20)
1 site		 T A A C T A T A A C G G T C C T A A G G T A G C G A A T T G A T A T T G C C A G G A T T C C A T C G C T
I-CeuI is a homing endonuclease that can recognize a variety of similar recognition sequences.
MfeI  (184)
1 site		 C A A T T G G T T A A C
MluI  (251)
1 site		 A C G C G T T G C G C A
HincII  (257)
1 site		 G T Y R A C C A R Y T G
SpeI  (272)
1 site		 A C T A G T T G A T C A
NdeI  (507)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
BmrI  (562)
1 site		 A C T G G G ( N ) 4 N T G A C C C ( N ) 4
The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the absence of magnesium.
BsaAI  (613)
1 site		 Y A C G T R R T G C A Y
SnaBI  (613)
1 site		 T A C G T A A T G C A T
NcoI  (633)
1 site		 C C A T G G G G T A C C
StyI  (633)
1 site		 C C W W G G G G W W C C
Sticky ends from different StyI sites may not be compatible.
BsaI  (899)
1 site		 G G T C T C N C C A G A G N ( N ) 4
Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
NheI  (918)
1 site		 G C T A G C C G A T C G
BmtI  (922)
1 site		 G C T A G C C G A T C G
DraI  (927)
1 site		 T T T A A A A A A T T T
PmeI  (927)
1 site		 G T T T A A A C C A A A T T T G
PspOMI  (932)
1 site		 G G G C C C C C C G G G
EcoO109I  (933)
1 site		 R G G N C C Y Y C C N G G R
Sticky ends from different EcoO109I sites may not be compatible.
ApaI  (936)
1 site		 G G G C C C C C C G G G
ApaI can be used between 25°C and 37°C.
XbaI  (938)
1 site		 T C T A G A A G A T C T
EagI  (950)
1 site		 C G G C C G G C C G G C
NotI  (950)
1 site		 G C G G C C G C C G C C G G C G
BstXI  (963)
1 site		 C C A N N N N N N T G G G G T N N N N N N A C C
Sticky ends from different BstXI sites may not be compatible.
Acc65I  (981)
1 site		 G G T A C C C C A T G G
KpnI  (985)
1 site		 G G T A C C C C A T G G
AflII  (990)
1 site		 C T T A A G G A A T T C
PsiI  (1026)
1 site		 T T A T A A A A T A T T
PstI  (1121)
1 site		 C T G C A G G A C G T C
EcoRI  (1127)
1 site		 G A A T T C C T T A A G
PI-SceI  (1147)
1 site		 A T C T A T G T C G G G T G C G G A G A A A G A G G T A A T G A A A T G G T A G A T A C A G C C C A C G C C T C T T T C T C C A T T A C T T T A C C
PI-SceI  is a homing endonuclease that can recognize a variety of similar recognition sequences.
BspQI  (1248)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different BspQI sites may not be compatible.
SapI  (1248)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
PciI  (1364)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
NspI  (1368)
1 site		 R C A T G Y Y G T A C R
DrdI  (1472)
1 site		 G A C N N N N N N G T C C T G N N N N N N C A G
Sticky ends from different DrdI sites may not be compatible.
BseYI  (1668)
1 site		 C C C A G C G G G T C G
After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
PspFI  (1672)
1 site		 C C C A G C G G G T C G
AcuI  (1912)
1 site		 C T G A A G ( N ) 14 N N G A C T T C ( N ) 14
Cleavage may be enhanced when more than one copy of the AcuI recognition sequence is present.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
Eco57MI  (1912)
1 site		 C T G R A G ( N ) 14 N N G A C Y T C ( N ) 14
Sticky ends from different Eco57MI sites may not be compatible.
After cleavage, Eco57MI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
KanR
2710 .. 3525  =  816 bp
271 amino acids  =  31.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin
KanR
2710 .. 3525  =  816 bp
271 amino acids  =  31.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin
ori
1425 .. 2013  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
1425 .. 2013  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
CMV enhancer
258 .. 637  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
258 .. 637  =  380 bp
human cytomegalovirus immediate early enhancer
CMV promoter
638 .. 841  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
638 .. 841  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
SV40 poly(A) signal
1007 .. 1088  =  82 bp
SV40 polyadenylation signal
SV40 poly(A) signal
1007 .. 1088  =  82 bp
SV40 polyadenylation signal
MCS
918 .. 995  =  78 bp
multiple cloning site
MCS
918 .. 995  =  78 bp
multiple cloning site
T7 promoter
886 .. 904  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
886 .. 904  =  19 bp
promoter for bacteriophage T7 RNA polymerase
stop codons
996 .. 1006  =  11 bp
stop codons in all three reading frames
stop codons
996 .. 1006  =  11 bp
stop codons in all three reading frames
ORF:  2263 .. 2502  =  240 bp
ORF:  79 amino acids  =  8.7 kDa
ORF:  1029 .. 1265  =  237 bp
ORF:  78 amino acids  =  9.0 kDa
ORF:  2710 .. 3525  =  816 bp
ORF:  271 amino acids  =  31.0 kDa
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Download pShuttle2.dna file

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Individual Sequences & Maps

pAAV-AC-GFP
pLenti6.2 N-Lumio V5-DEST
pLVX-rHom-1
pRetroX-Tet3G
pAAV-AC-Myc-DDK
pLenti6.2 V5-DEST
pLVX-rHom-Nuc1
pRetroX-TetOne
pAAV-AC-RFP
pLenti6.3 TO V5-DEST
pLVX-rHom-Sec1
pRetroX-TetOne-Puro
pAAV2-EF1a-tGFP-WPRE
pLenti6.3 V5-DEST
pLVX-Tet-Off Advanced
pRetroX-Tight-Hyg
pAd BLOCK-iT-DEST
pLenti6.4 R4R2 V5-DEST
pLVX-Tet-On Advanced
pRetroX-Tight-Pur
pAd CMV V5-DEST
pLenti6 BLOCK-iT-DEST
pLVX-Tet3G
pRetroX-TRE3G
pAd PL-DEST
pLenti6 capTEV-CT-DEST
pLVX-TetOne
pRFP-C-RS
pAdenoX-CMV Linear
pLenti6 capTEV-NT-DEST1
pLVX-TetOne-Puro
pRFP-CB-shLenti
pAdenoX-DsRed-Express Linear
pLenti6 TR
pLVX-Tight-Puro
pRS
pAdenoX-PRLS Linear
pLenti6 UbC V5-DEST
pLVX-TRE3G
pRSGC1-U6-sg-HTS6-CMV-Cas9-2A-Puro
pAdenoX-PRLS-DsRed-Express Linear
pLenti6 V5-DEST
pLVX-TRE3G-Hom1
pRSGC2-U6-sg-HTS6-UbiC-Cas9-2A-Puro
pAdenoX-PRLS-ZsGreen1 Linear
pLenti7.3 V5-DEST
pLVX-TRE3G-IRES
pRSI12-U6-sh-HTS4-UbiC-TagRFP-2A-Puro
pAdenoX-Tet3G Linear
pLEX-MCS
pLVX-TRE3G-mCherry
pRSI16-U6-sh-HTS6-UbiC-TagRFP-2A-Puro
pAdenoX-ZsGreen1 Linear
pLHCX
pLVX-TRE3G-ZsGreen1
pRSI16cb-U6-sh-13kCB18-HTS6-UbiC-TagRFP-2A-Puro
pB-RS
pLKO.1
pLX301
pRSI17-U6-sh-HTS6-UbiC-TagGFP2-2A-Puro
pBABE-Hygro
pLKO.1 puro
pLX302
pRSI17cb-U6-sh-13kCB18-HTS6-UbiC-TagGFP2-2A-Puro
pBABE-Neo
pLMN-ZsGreen-Neomycin
pLX303
pRSIT12-U6Tet-sh-HTS4-CMV-TetRep-2A-TagRFP-2A-Puro
pBABE-Puro
pLMPd-Ametrine
pLX304
pRSIT16-U6Tet-sh-HTS6-CMV-TetRep-2A-TagRFP-2A-Puro
pBABE-Zeo
pLNCX2
pLX304-V5-Blast-Empty
pRSIT17-U6Tet-sh-HTS6-CMV-TetRep-2A-TagGFP2-2A-Puro
pCMV-VSV-G
pLP VSVG
pLXIN
pRSITEP-U6Tet-sh-noHTS-EF1-TetRep-2A-Puro
pGFP-B-RS
pLP1
pLXSN
pRSITUR-U6Tet-sh-HTS3-UbiC-TetRep-2A-TagRFP
pGFP-C-shLenti
pLP2
pMD2.G
pShuttle2
pGFP-V-RS
pLPCX
pMSCV16URP-U6-sh-HTS6-UbiC-TagRFP-2A-Puro
pSIREN-RetroQ
pGIPZ
pLVX-EF1alpha-IRES-mCherry
pMSCVhyg
pSIREN-RetroQ-DsRed-Express
pLenti-C-GFP
pLVX-EF1alpha-IRES-Puro
pMSCVneo
pSIREN-RetroQ-TetH
pLenti-C-mGFP
pLVX-EF1alpha-IRES-ZsGreen1
pMSCVpuro
pSIREN-RetroQ-TetP
pLenti-C-Myc-DDK
pLVX-EF1alpha-Tet3G
pQC-tTS-IN
pSIREN-RetroQ-ZsGreen1
pLenti-C-Myc-DDK-IRES-BSD
pLVX-Het-1
pQCXIH
psPAX2
pLenti-C-Myc-DDK-IRES-GFP
pLVX-Het-2
pQCXIN
pTRE3G-Hom1
pLenti-C-Myc-DDK-IRES-Neo
pLVX-Het-Mem1
pQCXIP
pTRIPZ
pLenti-C-Myc-DDK-IRES-Puro
pLVX-Het-Nuc1
pQCXIX
ptTS-Neo
pLenti-C-Myc-DDK-IRES-tRFP
pLVX-Hom-1
pRetro-Lib
pZIP-hCMV-RFP-Puro
pLenti-C-tRFP
pLVX-Hom-Mem1
pRetroQ-AcGFP1-C1
pZIP-hCMV-ZSGreen-Puro
pLenti-C-tYFP
pLVX-Hom-Nuc1
pRetroQ-AcGFP1-N1
pZIP-hEF1-alpha-RFP-Puro
pLenti-EF1a-C-mGFP
pLVX-IRES-Hyg
pRetroQ-DsRed-Monomer-C1
pZIP-hEF1-alpha-ZsGreen-Puro
pLenti-EF1a-C-Myc-DDK-IRES-Puro
pLVX-IRES-mCherry
pRetroQ-DsRed-Monomer-N1
pZIP-mCMV-RFP-Puro
pLenti-EF1a-C-tGFP
pLVX-IRES-Neo
pRetroQ-mCherry-C1
pZIP-mCMV-ZsGreen-Puro
pLenti-N-tGFP
pLVX-IRES-Puro
pRetroQ-mCherry-N1
pZIP-mEF1-alpha-RFP-Puro
pLenti-N-tRFP
pLVX-IRES-tdTomato
pRetroX-IRES-DsRedExpress
pZIP-mEF1-alpha-ZsGreen-Puro
pLenti-N-tYFP
pLVX-IRES-ZsGreen1
pRetroX-IRES-ZsGreen1
pZIP-SFFV-RFP-Puro
pLenti3.3 TR
pLVX-PTuner
pRetroX-PTuner
pZIP-SFFV-ZsGreen-Puro
pLenti4 BLOCK-iT-DEST
pLVX-PTuner Green
pRetroX-PTuner IRES
pZIP-TRE3G-ZsGreen-Puro
pLenti4 V5-DEST
pLVX-PTunerC
pRetroX-Tet-Off Advanced
pSIREN-Shuttle (linearized)
pLenti6.2 C-Lumio V5-DEST
pLVX-Puro
pRetroX-Tet-On Advanced
scramble shRNA

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Lucigen Vectors
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