pLVX-TRE3G-ZsGreen1

Lentiviral vector for doxycycline-inducible expression of a gene together with the ZsGreen1 fluorescent protein.

Sequence Author: Clontech (TaKaRa)

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SgrDI (8732) SspI (8615) PvuI (8181) FspI (8033) DrdI (7029) BmtI (6537) NheI (6533) CsiI - SexAI * (4972) MscI * (4780) BstEII (4539) XcmI (8957) NruI * (833) MfeI (1189) BbvCI (1424) AleI (1577) KflI (1934) BspDI - ClaI (2180) PaeR7I - PspXI - XhoI (2203) BclI * (2646) SgrAI (2651) BsrGI (3114) SfiI (3267) PspOMI (3389) ApaI (3393) AvrII (3427) PaqCI (3615) TspMI - XmaI (3857) SmaI (3859) MluI (3863) NdeI (3870) EcoRI (3875) SphI (3924) AgeI (3995) SpeI (4130) BlpI (4158) PasI (4454) BsiWI (4461) RsrII (4521) pLVX-TRE3G-ZsGreen1 9100 bp
SgrDI  (8732)
1 site
C G T C G A C G G C A G C T G C
SspI  (8615)
1 site
A A T A T T T T A T A A
PvuI  (8181)
1 site
C G A T C G G C T A G C
FspI  (8033)
1 site
T G C G C A A C G C G T
DrdI  (7029)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
BmtI  (6537)
1 site
G C T A G C C G A T C G
NheI  (6533)
1 site
G C T A G C C G A T C G
CsiI  (4972)
1 site
A C C W G G T T G G W C C A

Sticky ends from different CsiI sites may not be compatible.
SexAI  (4972)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
MscI  (4780)
1 site
T G G C C A A C C G G T
* Blocked by Dcm methylation.
BstEII  (4539)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
XcmI  (8957)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N