pLVX-rHom-Sec1

Lentiviral vector encoding four FKBP homodimerizer domains, for creating ER-localized oligomers that can be dissociated with a drug. 

Sequence Author: Clontech (TaKaRa)

|Download SnapGene Viewer
No matches
XcmI (9407) SgrDI (9182) SspI (9065) PvuI (8631) FspI (8483) AhdI (8261) DrdI (7479) BmtI (6987) NheI (6983) Bsu36I (6193) PstI (6077) MluI (5459) CsiI - SexAI * (5426) MscI * (5234) BstEII (4993) RsrII (4975) BsiWI (4915) PflFI - Tth111I (4901) BmgBI (4796) NruI * (833) FseI (1150) EcoNI (1170) MfeI (1189) BbvCI - Bpu10I (1424) AleI (1577) KflI - PpuMI (1934) BspDI - ClaI (2180) NdeI (2374) CMV promoter EcoRI (2804) 5' MCS PaeR7I - XhoI (2810) XbaI (2900) NotI (4228) BamHI (4235) PspOMI (4366) ApaI (4370) AvrII (4404) pLVX-rHom-Sec1 9550 bp
XcmI  (9407)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
SgrDI  (9182)
1 site
C G T C G A C G G C A G C T G C
SspI  (9065)
1 site
A A T A T T T T A T A A
PvuI  (8631)
1 site
C G A T C G G C T A G C
FspI  (8483)
1 site
T G C G C A A C G C G T
AhdI  (8261)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
DrdI  (7479)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
BmtI  (6987)
1 site
G C T A G C C G A T C G
NheI  (6983)
1 site
G C T A G C C G A T C G
Bsu36I  (6193)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
PstI  (6077)
1 site
C T G C A G G A C G T C
MluI  (5459)
1 site
A C G C G T T G C G C A
CsiI  (5426)
1 site
A C C W G G T T G G W C C A

Sticky ends from different CsiI sites may not be compatible.
SexAI  (5426)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
MscI  (5234)
1 site
T G G C C A A C C G G T
* Blocked by Dcm methylation.
BstEII  (4993)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
RsrII  (4975)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsiWI  (4915)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
PflFI  (4901)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (4901)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BmgBI  (4796)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
NruI  (833)
1 site
T C G C G A A G C G C T
* Blocked by Dam methylation.
FseI  (1150)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
EcoNI  (1170)
1 site
C C T N N N N