pRSIT16-U6Tet-sh-HTS6-CMV-TetRep-2A-TagRFP-2A-Puro

Lentiviral vector for inducible barcoded shRNA libraries, with an HTS6 cassette for high-throughput sequencing plus TagRFP and puromycin resistance markers.

Sequence Author: Cellecta

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HomePlasmidsViral Expression & Packaging VectorspRSIT16-U6Tet-sh-HTS6-CMV-TetRep-2A-TagRFP-2A-Puro
MluI (1) SspI (8086) ScaI (7762) PciI (6389) SfiI (5967) AanI (5738) AanI (5682) KpnI (5347) Acc65I (5343) NaeI (5266) NgoMIV (5264) HincII (4748) SalI (4746) SexAI * (4707) EagI (4588) BstEII (4274) SphI (96) NruI * (612) MfeI (964) BbvCI - Bpu10I (1199) AleI (1352) PpuMI - SanDI (1709) BspDI - ClaI (1798) BbsI - BpiI (2065) cloning site BbsI - BpiI (2091) PaeR7I - PspXI - XhoI (2114) EcoRI (2281) SnaBI (2362) XbaI * (2654) BstXI (2750) BlpI (3208) BclI * (3238) BsrGI (3400) PshAI (3990) NheI (4062) BmtI (4066) PflFI - Tth111I (4182) BsiWI (4196) RsrII (4256) pRSIT16-U6Tet-sh-HTS6-CMV- TetRep-2A-TagRFP-2A-Puro 8673 bp