pRetro-Lib

Retroviral vector for delivery and expression of libraries or individual genes.

Sequence Author: Clontech (TaKaRa)

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HomePlasmidsViral Expression & Packaging VectorspRetro-Lib
SacII (4871) SspI (4529) XmnI (4324) ScaI (4205) PvuI (4095) FspI (3947) AseI (3897) BsrFI (3805) PspFI (3140) BseYI (3136) AflIII - PciI (2832) BspQI - SapI (2716) NdeI (2655) BstZ17I (2605) AccI (2604) AscI (443) PshAI (577) SpeI (726) AfeI (989) BstEII (1153) CsiI - SexAI * (1281) BsrGI (1348) 5' sequencing primer (1444 .. 1463) EcoRI (1470) SbfI (1496) HpaI (1564) PsiI (1584) BspDI - ClaI (1719) 3' sequencing primer (1760 .. 1785) XbaI (2060) BsaAI (2586) pRetro-Lib 4891 bp
SacII  (4871)
1 site		 C C G C G G G G C G C C
Efficient cleavage requires at least two copies of the SacII recognition sequence.
SspI  (4529)
1 site		 A A T A T T T T A T A A
XmnI  (4324)
1 site		 G A A N N N N T T C C T T N N N N A A G
ScaI  (4205)
1 site		 A G T A C T T C A T G A
PvuI  (4095)
1 site		 C G A T C G G C T A G C
FspI  (3947)
1 site		 T G C G C A A C G C G T
AseI  (3897)
1 site		 A T T A A T T A A T T A
BsrFI  (3805)
1 site		 R C C G G Y Y G G C C R
Cleavage may be enhanced when more than one copy of the BsrFI recognition sequence is present.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
PspFI  (3140)
1 site		 C C C A G C G G G T C G
BseYI  (3136)
1 site		 C C C A G C G G G T C G
After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
AflIII  (2832)
1 site		 A C R Y G T T G Y R C A
Sticky ends from different AflIII sites may not be compatible.
PciI  (2832)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
BspQI  (2716)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different BspQI sites may not be compatible.
SapI  (2716)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
NdeI  (2655)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
BstZ17I  (2605)
1 site		 G T A T A C C A T A T G
AccI  (2604)
1 site		 G T M K A C C A K M T G
Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
AscI  (443)
1 site		 G G C G C G C C C C G C G C G G
PshAI  (577)
1 site		 G A C N N N N G T C C T G N N N N C A G
PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
SpeI  (726)
1 site		 A C T A G T T G A T C A
AfeI  (989)
1 site		 A G C G C T T C G C G A
BstEII  (1153)
1 site		 G G T N A C C C C A N T G G
Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
CsiI  (1281)
1 site		 A C C W G G T T G G W C C A
Sticky ends from different CsiI sites may not be compatible.
SexAI  (1281)
1 site		 A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
BsrGI  (1348)
1 site		 T G T A C A A C A T G T
BsrGI is typically used at 37°C, but is even more active at 60°C.
EcoRI  (1470)
1 site		 G A A T T C C T T A A G
SbfI  (1496)
1 site		 C C T G C A G G G G A C G T C C
HpaI  (1564)
1 site		 G T T A A C C A A T T G
PsiI  (1584)
1 site		 T T A T A A A A T A T T
BspDI  (1719)
1 site		 A T C G A T T A G C T A
ClaI  (1719)
1 site		 A T C G A T T A G C T A
XbaI  (2060)
1 site		 T C T A G A A G A T C T
BsaAI  (2586)
1 site		 Y A C G T R R T G C A Y
5' sequencing primer
20-mer  /  55% GC
1 binding site
1444 .. 1463  =  20 annealed bases
Tm  =  56°C
3' sequencing primer
26-mer  /  54% GC
1 binding site
1760 .. 1785  =  26 annealed bases
Tm  =  64°C
AmpR
3652 .. 4512  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
   Segment 2:  
   3652 .. 4443  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3652 .. 4512  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
   Segment 1:  signal sequence  
   4444 .. 4512  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3652 .. 4512  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
3' LTR
1763 .. 2356  =  594 bp
long terminal repeat from Moloney murine leukemia virus
3' LTR
1763 .. 2356  =  594 bp
long terminal repeat from Moloney murine leukemia virus
5' LTR
1 .. 589  =  589 bp
long terminal repeat from Moloney murine leukemia virus
5' LTR
1 .. 589  =  589 bp
long terminal repeat from Moloney murine leukemia virus
ori
2893 .. 3481  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
2893 .. 3481  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
gag (truncated)
1052 .. 1468  =  417 bp
truncated MMLV gag gene lacking the start codon
gag (truncated)
1052 .. 1468  =  417 bp
truncated MMLV gag gene lacking the start codon
MMLV Ψ
652 .. 851  =  200 bp
packaging signal of Moloney murine leukemia virus (MMLV)
MMLV Ψ
652 .. 851  =  200 bp
packaging signal of Moloney murine leukemia virus (MMLV)
Stuffer fragment
1503 .. 1694  =  192 bp
Stuffer fragment
1503 .. 1694  =  192 bp
AmpR promoter
4513 .. 4617  =  105 bp
AmpR promoter
4513 .. 4617  =  105 bp
MCS A
1470 .. 1502  =  33 bp
multiple cloning site
MCS A
1470 .. 1502  =  33 bp
multiple cloning site
MCS B
1695 .. 1723  =  29 bp
multiple cloning site
MCS B
1695 .. 1723  =  29 bp
multiple cloning site
ORF:  3782 .. 4048  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  2180 .. 2470  =  291 bp
ORF:  96 amino acids  =  10.5 kDa
ORF:  739 .. 999  =  261 bp
ORF:  86 amino acids  =  9.5 kDa
ORF:  3652 .. 4512  =  861 bp
ORF:  286 amino acids  =  31.5 kDa
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Individual Sequences & Maps

pAAV-AC-GFP
pLenti6.2 N-Lumio V5-DEST
pLVX-rHom-1
pRetroX-Tet3G
pAAV-AC-Myc-DDK
pLenti6.2 V5-DEST
pLVX-rHom-Nuc1
pRetroX-TetOne
pAAV-AC-RFP
pLenti6.3 TO V5-DEST
pLVX-rHom-Sec1
pRetroX-TetOne-Puro
pAAV2-EF1a-tGFP-WPRE
pLenti6.3 V5-DEST
pLVX-Tet-Off Advanced
pRetroX-Tight-Hyg
pAd BLOCK-iT-DEST
pLenti6.4 R4R2 V5-DEST
pLVX-Tet-On Advanced
pRetroX-Tight-Pur
pAd CMV V5-DEST
pLenti6 BLOCK-iT-DEST
pLVX-Tet3G
pRetroX-TRE3G
pAd PL-DEST
pLenti6 capTEV-CT-DEST
pLVX-TetOne
pRFP-C-RS
pAdenoX-CMV Linear
pLenti6 capTEV-NT-DEST1
pLVX-TetOne-Puro
pRFP-CB-shLenti
pAdenoX-DsRed-Express Linear
pLenti6 TR
pLVX-Tight-Puro
pRS
pAdenoX-PRLS Linear
pLenti6 UbC V5-DEST
pLVX-TRE3G
pRSGC1-U6-sg-HTS6-CMV-Cas9-2A-Puro
pAdenoX-PRLS-DsRed-Express Linear
pLenti6 V5-DEST
pLVX-TRE3G-Hom1
pRSGC2-U6-sg-HTS6-UbiC-Cas9-2A-Puro
pAdenoX-PRLS-ZsGreen1 Linear
pLenti7.3 V5-DEST
pLVX-TRE3G-IRES
pRSI12-U6-sh-HTS4-UbiC-TagRFP-2A-Puro
pAdenoX-Tet3G Linear
pLEX-MCS
pLVX-TRE3G-mCherry
pRSI16-U6-sh-HTS6-UbiC-TagRFP-2A-Puro
pAdenoX-ZsGreen1 Linear
pLHCX
pLVX-TRE3G-ZsGreen1
pRSI16cb-U6-sh-13kCB18-HTS6-UbiC-TagRFP-2A-Puro
pB-RS
pLKO.1
pLX301
pRSI17-U6-sh-HTS6-UbiC-TagGFP2-2A-Puro
pBABE-Hygro
pLKO.1 puro
pLX302
pRSI17cb-U6-sh-13kCB18-HTS6-UbiC-TagGFP2-2A-Puro
pBABE-Neo
pLMN-ZsGreen-Neomycin
pLX303
pRSIT12-U6Tet-sh-HTS4-CMV-TetRep-2A-TagRFP-2A-Puro
pBABE-Puro
pLMPd-Ametrine
pLX304
pRSIT16-U6Tet-sh-HTS6-CMV-TetRep-2A-TagRFP-2A-Puro
pBABE-Zeo
pLNCX2
pLX304-V5-Blast-Empty
pRSIT17-U6Tet-sh-HTS6-CMV-TetRep-2A-TagGFP2-2A-Puro
pCMV-VSV-G
pLP VSVG
pLXIN
pRSITEP-U6Tet-sh-noHTS-EF1-TetRep-2A-Puro
pGFP-B-RS
pLP1
pLXSN
pRSITUR-U6Tet-sh-HTS3-UbiC-TetRep-2A-TagRFP
pGFP-C-shLenti
pLP2
pMD2.G
pShuttle2
pGFP-V-RS
pLPCX
pMSCV16URP-U6-sh-HTS6-UbiC-TagRFP-2A-Puro
pSIREN-RetroQ
pGIPZ
pLVX-EF1alpha-IRES-mCherry
pMSCVhyg
pSIREN-RetroQ-DsRed-Express
pLenti-C-GFP
pLVX-EF1alpha-IRES-Puro
pMSCVneo
pSIREN-RetroQ-TetH
pLenti-C-mGFP
pLVX-EF1alpha-IRES-ZsGreen1
pMSCVpuro
pSIREN-RetroQ-TetP
pLenti-C-Myc-DDK
pLVX-EF1alpha-Tet3G
pQC-tTS-IN
pSIREN-RetroQ-ZsGreen1
pLenti-C-Myc-DDK-IRES-BSD
pLVX-Het-1
pQCXIH
psPAX2
pLenti-C-Myc-DDK-IRES-GFP
pLVX-Het-2
pQCXIN
pTRE3G-Hom1
pLenti-C-Myc-DDK-IRES-Neo
pLVX-Het-Mem1
pQCXIP
pTRIPZ
pLenti-C-Myc-DDK-IRES-Puro
pLVX-Het-Nuc1
pQCXIX
ptTS-Neo
pLenti-C-Myc-DDK-IRES-tRFP
pLVX-Hom-1
pRetro-Lib
pZIP-hCMV-RFP-Puro
pLenti-C-tRFP
pLVX-Hom-Mem1
pRetroQ-AcGFP1-C1
pZIP-hCMV-ZSGreen-Puro
pLenti-C-tYFP
pLVX-Hom-Nuc1
pRetroQ-AcGFP1-N1
pZIP-hEF1-alpha-RFP-Puro
pLenti-EF1a-C-mGFP
pLVX-IRES-Hyg
pRetroQ-DsRed-Monomer-C1
pZIP-hEF1-alpha-ZsGreen-Puro
pLenti-EF1a-C-Myc-DDK-IRES-Puro
pLVX-IRES-mCherry
pRetroQ-DsRed-Monomer-N1
pZIP-mCMV-RFP-Puro
pLenti-EF1a-C-tGFP
pLVX-IRES-Neo
pRetroQ-mCherry-C1
pZIP-mCMV-ZsGreen-Puro
pLenti-N-tGFP
pLVX-IRES-Puro
pRetroQ-mCherry-N1
pZIP-mEF1-alpha-RFP-Puro
pLenti-N-tRFP
pLVX-IRES-tdTomato
pRetroX-IRES-DsRedExpress
pZIP-mEF1-alpha-ZsGreen-Puro
pLenti-N-tYFP
pLVX-IRES-ZsGreen1
pRetroX-IRES-ZsGreen1
pZIP-SFFV-RFP-Puro
pLenti3.3 TR
pLVX-PTuner
pRetroX-PTuner
pZIP-SFFV-ZsGreen-Puro
pLenti4 BLOCK-iT-DEST
pLVX-PTuner Green
pRetroX-PTuner IRES
pZIP-TRE3G-ZsGreen-Puro
pLenti4 V5-DEST
pLVX-PTunerC
pRetroX-Tet-Off Advanced
pSIREN-Shuttle (linearized)
pLenti6.2 C-Lumio V5-DEST
pLVX-Puro
pRetroX-Tet-On Advanced
scramble shRNA

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Lucigen Vectors
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Mammalian Expression Vectors
Structural Genomics Vectors

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