pRSI16-U6-sh-HTS6-UbiC-TagRFP-2A-Puro

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No matches
MluI (1) SspI (7438) ScaI (7114) AhdI (6634) PciI (5741) SfiI (5319) AanI (5090) AanI (5034) KpnI (4699) Acc65I (4695) NaeI (4618) NgoMIV (4616) HincII (4100) SalI (4098) SphI (96) NruI * (612) EcoNI (945) MfeI (964) BbvCI (1199) PpuMI - SanDI (1709) BspDI - ClaI (1798) BsaAI (1928) BbsI - BpiI (2064) BbsI - BpiI (2090) PaeR7I - PspXI - XhoI (2113) EcoRI (2280) AgeI (2289) BstBI (2298) AfeI (2358) XbaI (2702) BsrGI (2764) Bsu36I (2980) PshAI (3354) BamHI (3426) PflFI - Tth111I (3534) BsiWI (3548) BspEI * (3605) RsrII (3608) BstEII (3626) EagI (3940) SexAI * (4059) pRSI16-U6-sh-HTS6- UbiC-TagRFP-2A-Puro 8025 bp
MluI  (1)
1 site
A C G C G T T G C G C A
SspI  (7438)
1 site
A A T A T T T T A T A A
ScaI  (7114)
1 site
A G T A C T T C A T G A
AhdI  (6634)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
PciI  (5741)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
SfiI  (5319)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
AanI  (5090)
2 sites
T T A T A A A A T A T T
AanI  (5034)
2 sites
T T A T A A A A T A T T
KpnI  (4699)
1 site
G G T A C C C C A T G G
Acc65I  (4695)
1 site
G G T A C C C C A T G G
NaeI  (4618)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (4616)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
HincII  (4100)
1 site
G T Y R A C C A R Y T G
SalI  (4098)
1 site
G T C G A C C A G C T G
SphI  (96)
1 site
G C A T G C C G T A C G
NruI  (612)
1 site
T C G C G A A G C G C T
* Blocked by Dam methylation.
EcoNI  (945)
1 site
C C T N N N N N A G G G G A N N