pRetroX-Tight-Hyg
Retroviral vector for expressing a gene with the Tet-On® Advanced or Tet-Off® Advanced system.
Sequence Author: Clontech (TaKaRa)
Explore Over 2.7k Plasmids: Viral Expression & Packaging Vectors | More Plasmid Sets
No matches
|
|
|
| ||
PciI is inhibited by nonionic detergents. |
| ||
Sticky ends from different BspQI sites may not be compatible. |
| ||
Sticky ends from different SapI sites may not be compatible.SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot. |
|
| ||
Efficient cleavage requires at least two copies of the SfiI recognition sequence. Sticky ends from different SfiI sites may not be compatible. |
|
| ||
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage. |
|
|
|
|
|
|
| ||
Sticky ends from different BstEII sites may not be compatible.BstEII is typically used at 60°C, but is 50% active at 37°C. |
|
| ||
PaeR7I does not recognize the sequence CTCTCGAG. |
|
|
| ||
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
|
| ||
Efficient cleavage requires at least two copies of the NgoMIV recognition sequence. |
| ||
Efficient cleavage requires at least two copies of the NaeI recognition sequence. |
| ||
| * Blocked by Dam methylation. |
| ||
| * Blocked by Dam methylation. |
|
|
|