Sticky ends from different BspQI sites may not be compatible.
SapI (4409) 1 site
Sticky ends from different SapI sites may not be compatible.SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
AvrII (4278) 1 site
SfiI (4231) 1 site
Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
AccI (3397) 1 site
Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.Sticky ends from different AccI sites may not be compatible.
SalI (3396) 1 site
BmtI (3307) 1 site
BtgZI (278) 1 site
Sticky ends from different BtgZI sites may not be compatible.After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.BtgZI is typically used at 60°C, but is 75% active at 37°C.
SnaBI (284) 1 site
AscI (542) 1 site
PshAI (676) 1 site
PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
AfeI (1088) 1 site
BglII (1576) 1 site
PspXI (1600) 1 site
BamHI (1925) 1 site
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
