pRetroX-Tight-Pur

Retroviral vector for expressing a gene with the Tet-On® Advanced or Tet-Off® Advanced system.

Sequence Author: Clontech (TaKaRa)

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CMV enhancer SspI (6222) XmnI (6017) ScaI (5898) PvuI (5788) FspI (5640) PciI (4525) BspQI - SapI (4409) AvrII (4278) SfiI (4231) AccI (3397) SalI (3396) BmtI (3307) BtgZI (278) SnaBI (284) AscI (542) PshAI (676) AfeI (1088) BglII (1576) PspXI (1600) BamHI (1925) NotI (1932) NgoMIV (1939) NaeI (1941) XbaI * (1945) NruI * (1953) MluI (1957) EcoRI (1963) AgeI (2083) BlpI (2246) BsmI (2404) BfuAI - BspMI (2482) BsiWI (2549) RsrII (2609) DraIII (3064) EcoRV (3161) PvuII (3204) NheI (3303) pRetroX-Tight-Pur 6567 bp
SspI  (6222)
1 site
A A T A T T T T A T A A
XmnI  (6017)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (5898)
1 site
A G T A C T T C A T G A
PvuI  (5788)
1 site
C G A T C G G C T A G C
FspI  (5640)
1 site
T G C G C A A C G C G T
PciI  (4525)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BspQI  (4409)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (4409)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
AvrII  (4278)
1 site
C C T A G G G G A T C C
SfiI  (4231)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
AccI  (3397)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
SalI  (3396)
1 site
G T C G A C C A G C T G
BmtI  (3307)
1 site
G C T A G C C G A T C G
BtgZI  (278)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 (