pLenti-EF1a-C-mGFP

Lentiviral PrecisionShuttle™ vector for expression driven by the EF-1α promoter of an ORF tagged at the C-terminus with TagGFP2 (mTagGFP).

Sequence Author: OriGene

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HomePlasmidsViral Expression & Packaging VectorspLenti-EF1a-C-mGFP
BsmBI (7037) MscI (6998) ScaI (6848) DrdI (6202) Bsu36I (5518) PmeI (4659) BsaBI * (4512) BstEII (4106) NotI (3915) BsiWI (3909) MluI (3906) RsrII (3900) AscI (3869) MreI - SgrAI (3865) AsiSI - SgfI (3863) BamHI (3831) SgrDI (1) AseI (259) CMV enhancer NdeI (486) SnaBI (592) BspQI - SapI (1482) SanDI (2314) XbaI (2613) AgeI (2702) BfuAI - BspMI (3148) EcoRI (3818) pLenti-EF1a-C-mGFP 7612 bp
BsmBI  (7037)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
MscI  (6998)
1 site		 T G G C C A A C C G G T
ScaI  (6848)
1 site		 A G T A C T T C A T G A
DrdI  (6202)
1 site		 G A C N N N N N N G T C C T G N N N N N N C A G
Sticky ends from different DrdI sites may not be compatible.
Bsu36I  (5518)
1 site		 C C T N A G G G G A N T C C
Sticky ends from different Bsu36I sites may not be compatible.
PmeI  (4659)
1 site		 G T T T A A A C C A A A T T T G
BsaBI  (4512)
1 site		 G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
BstEII  (4106)
1 site		 G G T N A C C C C A N T G G
Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
NotI  (3915)
1 site		 G C G G C C G C C G C C G G C G
BsiWI  (3909)
1 site		 C G T A C G G C A T G C
BsiWI is typically used at 55°C, but is 50% active at 37°C.
MluI  (3906)
1 site		 A C G C G T T G C G C A
RsrII  (3900)
1 site		 C G G W C C G G C C W G G C
Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
AscI  (3869)
1 site		 G G C G C G C C C C G C G C G G
MreI  (3865)
1 site		 C G C C G G C G G C G G C C G C
SgrAI  (3865)
1 site		 C R C C G G Y G G Y G G C C R C
Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
AsiSI  (3863)
1 site		 G C G A T C G C C G C T A G C G
SgfI  (3863)
1 site		 G C G A T C G C C G C T A G C G
BamHI  (3831)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
SgrDI  (1)
1 site		 C G T C G A C G G C A G C T G C
AseI  (259)
1 site		 A T T A A T T A A T T A
NdeI  (486)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (592)
1 site		 T A C G T A A T G C A T
BspQI  (1482)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different BspQI sites may not be compatible.
SapI  (1482)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
SanDI  (2314)
1 site		 G G G W C C C C C C W G G G
Sticky ends from different SanDI sites may not be compatible.
XbaI  (2613)
1 site		 T C T A G A A G A T C T
AgeI  (2702)
1 site		 A C C G G T T G G C C A
BfuAI  (3148)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (3148)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
EcoRI  (3818)
1 site		 G A A T T C C T T A A G
EF-1α promoter
2624 .. 3802  =  1179 bp
strong constitutive promoter for human elongation factor EF-1α
EF-1α promoter
2624 .. 3802  =  1179 bp
strong constitutive promoter for human elongation factor EF-1α
TagGFP2
3927 .. 4640  =  714 bp
238 amino acids  =  26.9 kDa
Product: monomeric green fluorescent protein, also known as mTagGFP
mammalian codon-optimized
TagGFP2
3927 .. 4640  =  714 bp
238 amino acids  =  26.9 kDa
Product: monomeric green fluorescent protein, also known as mTagGFP
mammalian codon-optimized
CmR
6820 .. 7479  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
6820 .. 7479  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
WPRE
4796 .. 5384  =  589 bp
woodchuck hepatitis virus posttranscriptional regulatory element
WPRE
4796 .. 5384  =  589 bp
woodchuck hepatitis virus posttranscriptional regulatory element
ori
6155 .. 6743  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
6155 .. 6743  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
CMV enhancer
237 .. 616  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
237 .. 616  =  380 bp
human cytomegalovirus immediate early enhancer
RRE
1683 .. 1916  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
RRE
1683 .. 1916  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
CMV promoter
618 .. 816  =  199 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
618 .. 816  =  199 bp
human cytomegalovirus (CMV) immediate early promoter
5' LTR (truncated)
834 .. 1014  =  181 bp
truncated 5' long terminal repeat (LTR) from HIV-1
5' LTR (truncated)
834 .. 1014  =  181 bp
truncated 5' long terminal repeat (LTR) from HIV-1
3' LTR (truncated)
5913 .. 6093  =  181 bp
truncated 3' long terminal repeat (LTR) from HIV-1
3' LTR (truncated)
5913 .. 6093  =  181 bp
truncated 3' long terminal repeat (LTR) from HIV-1
HIV-1 Ψ
1061 .. 1186  =  126 bp
packaging signal of human immunodeficiency virus type 1
HIV-1 Ψ
1061 .. 1186  =  126 bp
packaging signal of human immunodeficiency virus type 1
cPPT/CTS
2443 .. 2560  =  118 bp
central polypurine tract and central termination sequence of HIV-1
cPPT/CTS
2443 .. 2560  =  118 bp
central polypurine tract and central termination sequence of HIV-1
AmpR promoter
7480 .. 7584  =  105 bp
AmpR promoter
7480 .. 7584  =  105 bp
MCS
3818 .. 3921  =  104 bp
multiple cloning site
MCS
3818 .. 3921  =  104 bp
multiple cloning site
loxP
4707 .. 4740  =  34 bp
Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).
loxP
4707 .. 4740  =  34 bp
Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).
EF-1α intron A
2855 .. 3793  =  939 bp
intron upstream of the start codon of human EF-1α
EF-1α intron A
2855 .. 3793  =  939 bp
intron upstream of the start codon of human EF-1α
ORF:  1561 .. 2448  =  888 bp
ORF:  295 amino acids  =  33.8 kDa
ORF:  3259 .. 3492  =  234 bp
ORF:  77 amino acids  =  7.7 kDa
ORF:  4886 .. 5440  =  555 bp
ORF:  184 amino acids  =  20.5 kDa
ORF:  3180 .. 3617  =  438 bp
ORF:  145 amino acids  =  15.9 kDa
ORF:  3927 .. 4655  =  729 bp
ORF:  242 amino acids  =  27.3 kDa
ORF:  4899 .. 5939  =  1041 bp
ORF:  346 amino acids  =  38.3 kDa
ORF:  4199 .. 4438  =  240 bp
ORF:  79 amino acids  =  9.4 kDa
ORF:  6820 .. 7479  =  660 bp
ORF:  219 amino acids  =  25.7 kDa
ORF:  1395 .. 1856  =  462 bp
ORF:  153 amino acids  =  16.6 kDa
ORF:  2922 .. 3542  =  621 bp
ORF:  206 amino acids  =  23.0 kDa
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Individual Sequences & Maps

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pLenti6.2 N-Lumio V5-DEST
pLVX-rHom-1
pRetroX-Tet3G
pAAV-AC-Myc-DDK
pLenti6.2 V5-DEST
pLVX-rHom-Nuc1
pRetroX-TetOne
pAAV-AC-RFP
pLenti6.3 TO V5-DEST
pLVX-rHom-Sec1
pRetroX-TetOne-Puro
pAAV2-EF1a-tGFP-WPRE
pLenti6.3 V5-DEST
pLVX-Tet-Off Advanced
pRetroX-Tight-Hyg
pAd BLOCK-iT-DEST
pLenti6.4 R4R2 V5-DEST
pLVX-Tet-On Advanced
pRetroX-Tight-Pur
pAd CMV V5-DEST
pLenti6 BLOCK-iT-DEST
pLVX-Tet3G
pRetroX-TRE3G
pAd PL-DEST
pLenti6 capTEV-CT-DEST
pLVX-TetOne
pRFP-C-RS
pAdenoX-CMV Linear
pLenti6 capTEV-NT-DEST1
pLVX-TetOne-Puro
pRFP-CB-shLenti
pAdenoX-DsRed-Express Linear
pLenti6 TR
pLVX-Tight-Puro
pRS
pAdenoX-PRLS Linear
pLenti6 UbC V5-DEST
pLVX-TRE3G
pRSGC1-U6-sg-HTS6-CMV-Cas9-2A-Puro
pAdenoX-PRLS-DsRed-Express Linear
pLenti6 V5-DEST
pLVX-TRE3G-Hom1
pRSGC2-U6-sg-HTS6-UbiC-Cas9-2A-Puro
pAdenoX-PRLS-ZsGreen1 Linear
pLenti7.3 V5-DEST
pLVX-TRE3G-IRES
pRSI12-U6-sh-HTS4-UbiC-TagRFP-2A-Puro
pAdenoX-Tet3G Linear
pLEX-MCS
pLVX-TRE3G-mCherry
pRSI16-U6-sh-HTS6-UbiC-TagRFP-2A-Puro
pAdenoX-ZsGreen1 Linear
pLHCX
pLVX-TRE3G-ZsGreen1
pRSI16cb-U6-sh-13kCB18-HTS6-UbiC-TagRFP-2A-Puro
pB-RS
pLKO.1
pLX301
pRSI17-U6-sh-HTS6-UbiC-TagGFP2-2A-Puro
pBABE-Hygro
pLKO.1 puro
pLX302
pRSI17cb-U6-sh-13kCB18-HTS6-UbiC-TagGFP2-2A-Puro
pBABE-Neo
pLMN-ZsGreen-Neomycin
pLX303
pRSIT12-U6Tet-sh-HTS4-CMV-TetRep-2A-TagRFP-2A-Puro
pBABE-Puro
pLMPd-Ametrine
pLX304
pRSIT16-U6Tet-sh-HTS6-CMV-TetRep-2A-TagRFP-2A-Puro
pBABE-Zeo
pLNCX2
pLX304-V5-Blast-Empty
pRSIT17-U6Tet-sh-HTS6-CMV-TetRep-2A-TagGFP2-2A-Puro
pCMV-VSV-G
pLP VSVG
pLXIN
pRSITEP-U6Tet-sh-noHTS-EF1-TetRep-2A-Puro
pGFP-B-RS
pLP1
pLXSN
pRSITUR-U6Tet-sh-HTS3-UbiC-TetRep-2A-TagRFP
pGFP-C-shLenti
pLP2
pMD2.G
pShuttle2
pGFP-V-RS
pLPCX
pMSCV16URP-U6-sh-HTS6-UbiC-TagRFP-2A-Puro
pSIREN-RetroQ
pGIPZ
pLVX-EF1alpha-IRES-mCherry
pMSCVhyg
pSIREN-RetroQ-DsRed-Express
pLenti-C-GFP
pLVX-EF1alpha-IRES-Puro
pMSCVneo
pSIREN-RetroQ-TetH
pLenti-C-mGFP
pLVX-EF1alpha-IRES-ZsGreen1
pMSCVpuro
pSIREN-RetroQ-TetP
pLenti-C-Myc-DDK
pLVX-EF1alpha-Tet3G
pQC-tTS-IN
pSIREN-RetroQ-ZsGreen1
pLenti-C-Myc-DDK-IRES-BSD
pLVX-Het-1
pQCXIH
psPAX2
pLenti-C-Myc-DDK-IRES-GFP
pLVX-Het-2
pQCXIN
pTRE3G-Hom1
pLenti-C-Myc-DDK-IRES-Neo
pLVX-Het-Mem1
pQCXIP
pTRIPZ
pLenti-C-Myc-DDK-IRES-Puro
pLVX-Het-Nuc1
pQCXIX
ptTS-Neo
pLenti-C-Myc-DDK-IRES-tRFP
pLVX-Hom-1
pRetro-Lib
pZIP-hCMV-RFP-Puro
pLenti-C-tRFP
pLVX-Hom-Mem1
pRetroQ-AcGFP1-C1
pZIP-hCMV-ZSGreen-Puro
pLenti-C-tYFP
pLVX-Hom-Nuc1
pRetroQ-AcGFP1-N1
pZIP-hEF1-alpha-RFP-Puro
pLenti-EF1a-C-mGFP
pLVX-IRES-Hyg
pRetroQ-DsRed-Monomer-C1
pZIP-hEF1-alpha-ZsGreen-Puro
pLenti-EF1a-C-Myc-DDK-IRES-Puro
pLVX-IRES-mCherry
pRetroQ-DsRed-Monomer-N1
pZIP-mCMV-RFP-Puro
pLenti-EF1a-C-tGFP
pLVX-IRES-Neo
pRetroQ-mCherry-C1
pZIP-mCMV-ZsGreen-Puro
pLenti-N-tGFP
pLVX-IRES-Puro
pRetroQ-mCherry-N1
pZIP-mEF1-alpha-RFP-Puro
pLenti-N-tRFP
pLVX-IRES-tdTomato
pRetroX-IRES-DsRedExpress
pZIP-mEF1-alpha-ZsGreen-Puro
pLenti-N-tYFP
pLVX-IRES-ZsGreen1
pRetroX-IRES-ZsGreen1
pZIP-SFFV-RFP-Puro
pLenti3.3 TR
pLVX-PTuner
pRetroX-PTuner
pZIP-SFFV-ZsGreen-Puro
pLenti4 BLOCK-iT-DEST
pLVX-PTuner Green
pRetroX-PTuner IRES
pZIP-TRE3G-ZsGreen-Puro
pLenti4 V5-DEST
pLVX-PTunerC
pRetroX-Tet-Off Advanced
pSIREN-Shuttle (linearized)
pLenti6.2 C-Lumio V5-DEST
pLVX-Puro
pRetroX-Tet-On Advanced
scramble shRNA

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