pLVX-Het-Mem1

Lentiviral vector encoding FKBP heterodimerizer domains, for creating membrane-associated fusion proteins that can be dimerized with a drug. 

Sequence Author: Clontech (TaKaRa)

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HomePlasmidsViral Expression & Packaging VectorspLVX-Het-Mem1
XcmI (9125) SgrDI (8900) SspI (8783) FspI (8201) AhdI (7979) BmtI (6705) NheI (6701) StuI (6690) PfoI (5463) MluI (5177) RsrII (4559) BssHII (711) NruI * (833) FseI (1150) EcoNI (1170) MfeI (1189) BbvCI - Bpu10I (1424) KflI (1934) cPPT/CTS BspDI - ClaI (2180) PaeR7I - XhoI (2810) SpeI (2816) N-myristoylation signal AfeI (2861) XbaI (2864) TspMI - XmaI (2985) SmaI (2987) FKBP (DmrA) SphI (3283) FKBP (DmrA) NotI (3520) BamHI (3527) PspOMI (3658) ApaI (3662) AvrII (3696) PshAI (4178) AsiSI (4515) pLVX-Het-Mem1 9268 bp
XcmI  (9125)
1 site		 C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C
The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
SgrDI  (8900)
1 site		 C G T C G A C G G C A G C T G C
SspI  (8783)
1 site		 A A T A T T T T A T A A
FspI  (8201)
1 site		 T G C G C A A C G C G T
AhdI  (7979)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BmtI  (6705)
1 site		 G C T A G C C G A T C G
NheI  (6701)
1 site		 G C T A G C C G A T C G
StuI  (6690)
1 site		 A G G C C T T C C G G A
PfoI  (5463)
1 site		 T C C N G G A A G G N C C T
Sticky ends from different PfoI sites may not be compatible.
MluI  (5177)
1 site		 A C G C G T T G C G C A
RsrII  (4559)
1 site		 C G G W C C G G C C W G G C
Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BssHII  (711)
1 site		 G C G C G C C G C G C G
BssHII is typically used at 50°C, but is 75% active at 37°C.
NruI  (833)
1 site		 T C G C G A A G C G C T
* Blocked by Dam methylation.
FseI  (1150)
1 site		 G G C C G G C C C C G G C C G G
FseI gradually loses activity when stored at -20°C.
EcoNI  (1170)
1 site		 C C T N N N N N A G G G G A N N N N N T C C
The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
MfeI  (1189)
1 site		 C A A T T G G T T A A C
BbvCI  (1424)
1 site		 C C T C A G C G G A G T C G
Bpu10I  (1424)
1 site		 C C T N A G C G G A N T C G
Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
KflI  (1934)
1 site		 G G G W C C C C C C W G G G
Sticky ends from different KflI sites may not be compatible.
BspDI  (2180)
1 site		 A T C G A T T A G C T A
ClaI  (2180)
1 site		 A T C G A T T A G C T A
PaeR7I  (2810)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (2810)
1 site		 C T C G A G G A G C T C
SpeI  (2816)
1 site		 A C T A G T T G A T C A
AfeI  (2861)
1 site		 A G C G C T T C G C G A
XbaI  (2864)
1 site		 T C T A G A A G A T C T
TspMI  (2985)
1 site		 C C C G G G G G G C C C
XmaI  (2985)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (2987)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
SphI  (3283)
1 site		 G C A T G C C G T A C G
NotI  (3520)
1 site		 G C G G C C G C C G C C G G C G
BamHI  (3527)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
PspOMI  (3658)
1 site		 G G G C C C C C C G G G
ApaI  (3662)
1 site		 G G G C C C C C C G G G
ApaI can be used between 25°C and 37°C.
AvrII  (3696)
1 site		 C C T A G G G G A T C C
PshAI  (4178)
1 site		 G A C N N N N G T C C T G N N N N C A G
PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
AsiSI  (4515)
1 site		 G C G A T C G C C G C T A G C G
HygR
4157 .. 5176  =  1020 bp
339 amino acids  =  37.8 kDa
Product: aminoglycoside phosphotransferase from E. coli
confers resistance to hygromycin
HygR
4157 .. 5176  =  1020 bp
339 amino acids  =  37.8 kDa
Product: aminoglycoside phosphotransferase from E. coli
confers resistance to hygromycin
AmpR
7906 .. 8766  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   7906 .. 8697  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
7906 .. 8766  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   8698 .. 8766  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
7906 .. 8766  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
N-myristoylation signal
2822 .. 2863  =  42 bp
14 amino acids  =  1.5 kDa
Product: N-myristoylation signal from Src kinase
N-myristoylation signal
2822 .. 2863  =  42 bp
14 amino acids  =  1.5 kDa
Product: N-myristoylation signal from Src kinase
FKBP (DmrA)
2870 .. 3190  =  321 bp
107 amino acids  =  11.8 kDa
Product: human FK506-binding protein FKBP12
FKBP (DmrA)
2870 .. 3190  =  321 bp
107 amino acids  =  11.8 kDa
Product: human FK506-binding protein FKBP12
FKBP (DmrA)
3197 .. 3517  =  321 bp
107 amino acids  =  11.8 kDa
Product: human FK506-binding protein FKBP12
synthetic gene with alternative codons
FKBP (DmrA)
3197 .. 3517  =  321 bp
107 amino acids  =  11.8 kDa
Product: human FK506-binding protein FKBP12
synthetic gene with alternative codons
3' LTR
5986 .. 6619  =  634 bp
3' long terminal repeat (LTR) from HIV-1
3' LTR
5986 .. 6619  =  634 bp
3' long terminal repeat (LTR) from HIV-1
5' LTR
1 .. 634  =  634 bp
5' long terminal repeat (LTR) from HIV-1
5' LTR
1 .. 634  =  634 bp
5' long terminal repeat (LTR) from HIV-1
WPRE
5190 .. 5778  =  589 bp
woodchuck hepatitis virus posttranscriptional regulatory element
WPRE
5190 .. 5778  =  589 bp
woodchuck hepatitis virus posttranscriptional regulatory element
ori
7150 .. 7735  =  586 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
7150 .. 7735  =  586 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
IRES
3545 .. 4118  =  574 bp
internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)
IRES
3545 .. 4118  =  574 bp
internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)
CMV enhancer
2201 .. 2504  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
2201 .. 2504  =  304 bp
human cytomegalovirus immediate early enhancer
RRE
1303 .. 1536  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
RRE
1303 .. 1536  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
CMV promoter
2505 .. 2708  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
2505 .. 2708  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
SV40 poly(A) signal
8919 .. 9053  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
8919 .. 9053  =  135 bp
SV40 polyadenylation signal
HIV-1 Ψ
681 .. 806  =  126 bp
packaging signal of human immunodeficiency virus type 1
HIV-1 Ψ
681 .. 806  =  126 bp
packaging signal of human immunodeficiency virus type 1
cPPT/CTS
2028 .. 2144  =  117 bp
central polypurine tract and central termination sequence of HIV-1 (lacking the first T)
cPPT/CTS
2028 .. 2144  =  117 bp
central polypurine tract and central termination sequence of HIV-1 (lacking the first T)
AmpR promoter
8767 .. 8871  =  105 bp
AmpR promoter
8767 .. 8871  =  105 bp
3' MCS
3519 .. 3532  =  14 bp
multiple cloning site downstream of DmrA
3' MCS
3519 .. 3532  =  14 bp
multiple cloning site downstream of DmrA
5' MCS
2810 .. 2821  =  12 bp
multiple cloning site upstream of DmrA
5' MCS
2810 .. 2821  =  12 bp
multiple cloning site upstream of DmrA
ORF:  5293 .. 5589  =  297 bp
ORF:  98 amino acids  =  10.2 kDa
ORF:  1181 .. 2032  =  852 bp
ORF:  283 amino acids  =  32.7 kDa
ORF:  2822 .. 3541  =  720 bp
ORF:  239 amino acids  =  26.3 kDa
ORF:  4028 .. 5176  =  1149 bp
ORF:  382 amino acids  =  42.6 kDa
ORF:  8036 .. 8302  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  5280 .. 5807  =  528 bp
ORF:  175 amino acids  =  19.0 kDa
ORF:  4043 .. 4909  =  867 bp
ORF:  288 amino acids  =  32.7 kDa
ORF:  1015 .. 1476  =  462 bp
ORF:  153 amino acids  =  16.6 kDa
ORF:  2587 .. 2889  =  303 bp
ORF:  100 amino acids  =  10.9 kDa
ORF:  7906 .. 8766  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
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Individual Sequences & Maps

pAAV-AC-GFP
pLenti6.2 N-Lumio V5-DEST
pLVX-rHom-1
pRetroX-Tet3G
pAAV-AC-Myc-DDK
pLenti6.2 V5-DEST
pLVX-rHom-Nuc1
pRetroX-TetOne
pAAV-AC-RFP
pLenti6.3 TO V5-DEST
pLVX-rHom-Sec1
pRetroX-TetOne-Puro
pAAV2-EF1a-tGFP-WPRE
pLenti6.3 V5-DEST
pLVX-Tet-Off Advanced
pRetroX-Tight-Hyg
pAd BLOCK-iT-DEST
pLenti6.4 R4R2 V5-DEST
pLVX-Tet-On Advanced
pRetroX-Tight-Pur
pAd CMV V5-DEST
pLenti6 BLOCK-iT-DEST
pLVX-Tet3G
pRetroX-TRE3G
pAd PL-DEST
pLenti6 capTEV-CT-DEST
pLVX-TetOne
pRFP-C-RS
pAdenoX-CMV Linear
pLenti6 capTEV-NT-DEST1
pLVX-TetOne-Puro
pRFP-CB-shLenti
pAdenoX-DsRed-Express Linear
pLenti6 TR
pLVX-Tight-Puro
pRS
pAdenoX-PRLS Linear
pLenti6 UbC V5-DEST
pLVX-TRE3G
pRSGC1-U6-sg-HTS6-CMV-Cas9-2A-Puro
pAdenoX-PRLS-DsRed-Express Linear
pLenti6 V5-DEST
pLVX-TRE3G-Hom1
pRSGC2-U6-sg-HTS6-UbiC-Cas9-2A-Puro
pAdenoX-PRLS-ZsGreen1 Linear
pLenti7.3 V5-DEST
pLVX-TRE3G-IRES
pRSI12-U6-sh-HTS4-UbiC-TagRFP-2A-Puro
pAdenoX-Tet3G Linear
pLEX-MCS
pLVX-TRE3G-mCherry
pRSI16-U6-sh-HTS6-UbiC-TagRFP-2A-Puro
pAdenoX-ZsGreen1 Linear
pLHCX
pLVX-TRE3G-ZsGreen1
pRSI16cb-U6-sh-13kCB18-HTS6-UbiC-TagRFP-2A-Puro
pB-RS
pLKO.1
pLX301
pRSI17-U6-sh-HTS6-UbiC-TagGFP2-2A-Puro
pBABE-Hygro
pLKO.1 puro
pLX302
pRSI17cb-U6-sh-13kCB18-HTS6-UbiC-TagGFP2-2A-Puro
pBABE-Neo
pLMN-ZsGreen-Neomycin
pLX303
pRSIT12-U6Tet-sh-HTS4-CMV-TetRep-2A-TagRFP-2A-Puro
pBABE-Puro
pLMPd-Ametrine
pLX304
pRSIT16-U6Tet-sh-HTS6-CMV-TetRep-2A-TagRFP-2A-Puro
pBABE-Zeo
pLNCX2
pLX304-V5-Blast-Empty
pRSIT17-U6Tet-sh-HTS6-CMV-TetRep-2A-TagGFP2-2A-Puro
pCMV-VSV-G
pLP VSVG
pLXIN
pRSITEP-U6Tet-sh-noHTS-EF1-TetRep-2A-Puro
pGFP-B-RS
pLP1
pLXSN
pRSITUR-U6Tet-sh-HTS3-UbiC-TetRep-2A-TagRFP
pGFP-C-shLenti
pLP2
pMD2.G
pShuttle2
pGFP-V-RS
pLPCX
pMSCV16URP-U6-sh-HTS6-UbiC-TagRFP-2A-Puro
pSIREN-RetroQ
pGIPZ
pLVX-EF1alpha-IRES-mCherry
pMSCVhyg
pSIREN-RetroQ-DsRed-Express
pLenti-C-GFP
pLVX-EF1alpha-IRES-Puro
pMSCVneo
pSIREN-RetroQ-TetH
pLenti-C-mGFP
pLVX-EF1alpha-IRES-ZsGreen1
pMSCVpuro
pSIREN-RetroQ-TetP
pLenti-C-Myc-DDK
pLVX-EF1alpha-Tet3G
pQC-tTS-IN
pSIREN-RetroQ-ZsGreen1
pLenti-C-Myc-DDK-IRES-BSD
pLVX-Het-1
pQCXIH
psPAX2
pLenti-C-Myc-DDK-IRES-GFP
pLVX-Het-2
pQCXIN
pTRE3G-Hom1
pLenti-C-Myc-DDK-IRES-Neo
pLVX-Het-Mem1
pQCXIP
pTRIPZ
pLenti-C-Myc-DDK-IRES-Puro
pLVX-Het-Nuc1
pQCXIX
ptTS-Neo
pLenti-C-Myc-DDK-IRES-tRFP
pLVX-Hom-1
pRetro-Lib
pZIP-hCMV-RFP-Puro
pLenti-C-tRFP
pLVX-Hom-Mem1
pRetroQ-AcGFP1-C1
pZIP-hCMV-ZSGreen-Puro
pLenti-C-tYFP
pLVX-Hom-Nuc1
pRetroQ-AcGFP1-N1
pZIP-hEF1-alpha-RFP-Puro
pLenti-EF1a-C-mGFP
pLVX-IRES-Hyg
pRetroQ-DsRed-Monomer-C1
pZIP-hEF1-alpha-ZsGreen-Puro
pLenti-EF1a-C-Myc-DDK-IRES-Puro
pLVX-IRES-mCherry
pRetroQ-DsRed-Monomer-N1
pZIP-mCMV-RFP-Puro
pLenti-EF1a-C-tGFP
pLVX-IRES-Neo
pRetroQ-mCherry-C1
pZIP-mCMV-ZsGreen-Puro
pLenti-N-tGFP
pLVX-IRES-Puro
pRetroQ-mCherry-N1
pZIP-mEF1-alpha-RFP-Puro
pLenti-N-tRFP
pLVX-IRES-tdTomato
pRetroX-IRES-DsRedExpress
pZIP-mEF1-alpha-ZsGreen-Puro
pLenti-N-tYFP
pLVX-IRES-ZsGreen1
pRetroX-IRES-ZsGreen1
pZIP-SFFV-RFP-Puro
pLenti3.3 TR
pLVX-PTuner
pRetroX-PTuner
pZIP-SFFV-ZsGreen-Puro
pLenti4 BLOCK-iT-DEST
pLVX-PTuner Green
pRetroX-PTuner IRES
pZIP-TRE3G-ZsGreen-Puro
pLenti4 V5-DEST
pLVX-PTunerC
pRetroX-Tet-Off Advanced
pSIREN-Shuttle (linearized)
pLenti6.2 C-Lumio V5-DEST
pLVX-Puro
pRetroX-Tet-On Advanced
scramble shRNA

Plasmid Sets

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TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

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