pLVX-TRE3G-Hom1

Lentiviral vector encoding a doxycycline-inducible FKBP homodimerizer domain, for creating soluble fusion proteins that can be dimerized with a drug.

Sequence Author: Clontech (TaKaRa)

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No matches
XcmI (8014) SgrDI (7789) SspI (7672) PvuI (7238) FspI (7090) AhdI (6868) DrdI (6086) PciI (5978) BmtI (5594) NheI (5590) KpnI (4808) Acc65I (4804) Bsu36I (4800) NcoI (4487) PflMI (4320) NruI * (833) FseI (1150) EcoNI (1170) MfeI (1189) BbvCI (1424) AleI (1577) KflI (1934) BspDI - ClaI (2180) PaeR7I - PspXI - XhoI (2203) TRE3GV promoter BamHI (2556) PspOMI (2562) ApaI (2566) NotI (2569) ATG XbaI (2582) PaqCI (2584) TspMI - XmaI (2703) SmaI (2705) MluI (2909) NdeI (2916) EcoRI (2921) SphI (2981) AgeI (3052) SpeI (3187) BlpI (3215) PflFI - Tth111I (3504) PasI (3511) BsiWI (3518) RsrII (3578) BstEII (3596) MscI * (3837) CsiI - SexAI * (4029) pLVX-TRE3G-Hom1 8157 bp
XcmI  (8014)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
SgrDI  (7789)
1 site
C G T C G A C G G C A G C T G C
SspI  (7672)
1 site
A A T A T T T T A T A A
PvuI  (7238)
1 site
C G A T C G G C T A G C
FspI  (7090)
1 site
T G C G C A A C G C G T
AhdI  (6868)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
DrdI  (6086)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
PciI  (5978)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BmtI  (5594)
1 site
G C T A G C C G A T C G
NheI  (5590)
1 site
G C T A G C C G A T C G
KpnI  (4808)
1 site
G G T A C C C C A T G G
Acc65I  (4804)
1 site
G G T A C C C C A T G G
Bsu36I  (4800)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
NcoI  (4487)
1 site
C C A T G G G G T A C C
PflMI  (4320)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
NruI  (833)
1 site
T C G C G A A G C G C T
* Blocked by Dam methylation.
FseI  (1150)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
EcoNI  (1170)
1 site
C C T N N N N N A G G G G A N N N N N T