Explore Over 2.7k Plasmids: Viral Expression & Packaging Vectors | More Plasmid Sets
No matches
|
| ||
PciI is inhibited by nonionic detergents. |
| ||
Efficient cleavage requires at least two copies of the SfiI recognition sequence. Sticky ends from different SfiI sites may not be compatible. |
|
|
|
|
|
|
| ||
* Blocked by Dcm methylation. Sticky ends from different SexAI sites may not be compatible. |
| ||
Sticky ends from different BstEII sites may not be compatible.BstEII is typically used at 60°C, but is 50% active at 37°C. |
| ||
Efficient cleavage requires at least two copies of the RsrII recognition sequence. Sticky ends from different RsrII sites may not be compatible.For full activity, add fresh DTT. |
| ||
BsiWI is typically used at 55°C, but is 50% active at 37°C. |
| ||
The 1-base overhangs produced by PflFI may be hard to ligate.Sticky ends from different PflFI sites may not be compatible. |
| ||
The 1-base overhangs produced by Tth111I may be hard to ligate.Sticky ends from different Tth111I sites may not be compatible. |
| ||
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
|
| ||
FseI gradually loses activity when stored at -20°C. |
|