pLVX-rHom-Nuc1
Lentiviral vector encoding three FKBP homodimerizer domains, for creating nuclear oligomers that can be dissociated with a drug.
Sequence Author: Clontech (TaKaRa)
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The 1-base overhangs produced by XcmI may be hard to ligate.Sticky ends from different XcmI sites may not be compatible. |
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The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible. |
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Sticky ends from different DrdI sites may not be compatible. |
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Sticky ends from different Bsu36I sites may not be compatible. |
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Sticky ends from different CsiI sites may not be compatible. |
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| * Blocked by Dcm methylation. Sticky ends from different SexAI sites may not be compatible. |
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| * Blocked by Dcm methylation. |
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Sticky ends from different BstEII sites may not be compatible.BstEII is typically used at 60°C, but is 50% active at 37°C. |
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Efficient cleavage requires at least two copies of the RsrII recognition sequence. Sticky ends from different RsrII sites may not be compatible.For full activity, add fresh DTT. |
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| * Blocked by Dam methylation. |
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FseI gradually loses activity when stored at -20°C. |
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The 1-base overhangs produced by EcoNI may be hard to ligate.Sticky ends from different EcoNI sites may not be compatible. |
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Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present. This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.Sticky ends from different Bpu10I sites may not be compatible. |
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Sticky ends from different KflI sites may not be compatible. |
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Sticky ends from different PpuMI sites may not be compatible. |
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Prolonged incubation with NdeI may lead to removal of additional nucleotides. |
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PaeR7I does not recognize the sequence CTCTCGAG. |
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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ApaI can be used between 25°C and 37°C. |
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This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site. |
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The 1-base overhangs produced by PflFI may be hard to ligate.Sticky ends from different PflFI sites may not be compatible. |
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The 1-base overhangs produced by Tth111I may be hard to ligate.Sticky ends from different Tth111I sites may not be compatible. |
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BsiWI is typically used at 55°C, but is 50% active at 37°C. |
| ATG 2822 .. 2824 = 3 bp 1 amino acid = 149.2 Da Product: start codon |
| ATG 2822 .. 2824 = 3 bp 1 amino acid = 149.2 Da Product: start codon |
| SV40 NLS 2825 .. 2845 = 21 bp 7 amino acids = 883.1 Da Product: nuclear localization signal of SV40 large T antigen |
| SV40 NLS 2825 .. 2845 = 21 bp 7 amino acids = 883.1 Da Product: nuclear localization signal of SV40 large T antigen |
| DmrD 2852 .. 3172 = 321 bp 107 amino acids = 11.8 kDa Product: dimeric F36M mutant of FK506-binding protein FKBP12 dimerization can be reversed by ligand binding |
| DmrD 2852 .. 3172 = 321 bp 107 amino acids = 11.8 kDa Product: dimeric F36M mutant of FK506-binding protein FKBP12 dimerization can be reversed by ligand binding |
| DmrD 3179 .. 3499 = 321 bp 107 amino acids = 11.8 kDa Product: dimeric F36M mutant of FK506-binding protein FKBP12 dimerization can be reversed by ligand binding |
| DmrD 3179 .. 3499 = 321 bp 107 amino acids = 11.8 kDa Product: dimeric F36M mutant of FK506-binding protein FKBP12 dimerization can be reversed by ligand binding |
| DmrD 3506 .. 3826 = 321 bp 107 amino acids = 11.8 kDa Product: dimeric F36M mutant of FK506-binding protein FKBP12 dimerization can be reversed by ligand binding |
| DmrD 3506 .. 3826 = 321 bp 107 amino acids = 11.8 kDa Product: dimeric F36M mutant of FK506-binding protein FKBP12 dimerization can be reversed by ligand binding |
| AmpR 7798 .. 8658 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 2: 7798 .. 8589 = 792 bp 263 amino acids = 28.9 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| AmpR 7798 .. 8658 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 1: signal sequence 8590 .. 8658 = 69 bp 23 amino acids = 2.6 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| AmpR 7798 .. 8658 = 861 bp 286 amino acids = 31.6 kDa 2 segments Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| 3' LTR 5878 .. 6511 = 634 bp 3' long terminal repeat (LTR) from HIV-1 |
| 3' LTR 5878 .. 6511 = 634 bp 3' long terminal repeat (LTR) from HIV-1 |
| 3' LTR 1 .. 634 = 634 bp 3' long terminal repeat (LTR) from HIV-1 |
| 3' LTR 1 .. 634 = 634 bp 3' long terminal repeat (LTR) from HIV-1 |
| PuroR 4469 .. 5068 = 600 bp 199 amino acids = 21.5 kDa Product: puromycin N-acetyltransferase confers resistance to puromycin |
| PuroR 4469 .. 5068 = 600 bp 199 amino acids = 21.5 kDa Product: puromycin N-acetyltransferase confers resistance to puromycin |
| WPRE 5082 .. 5670 = 589 bp woodchuck hepatitis virus posttranscriptional regulatory element |
| WPRE 5082 .. 5670 = 589 bp woodchuck hepatitis virus posttranscriptional regulatory element |
| ori 7042 .. 7627 = 586 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
| ori 7042 .. 7627 = 586 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
| IRES 3863 .. 4436 = 574 bp internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV) |
| IRES 3863 .. 4436 = 574 bp internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV) |
| CMV enhancer 2201 .. 2504 = 304 bp human cytomegalovirus immediate early enhancer |
| CMV enhancer 2201 .. 2504 = 304 bp human cytomegalovirus immediate early enhancer |
| RRE 1303 .. 1536 = 234 bp The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm. |
| RRE 1303 .. 1536 = 234 bp The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm. |
| CMV promoter 2505 .. 2708 = 204 bp human cytomegalovirus (CMV) immediate early promoter |
| CMV promoter 2505 .. 2708 = 204 bp human cytomegalovirus (CMV) immediate early promoter |
| SV40 poly(A) signal 8811 .. 8945 = 135 bp SV40 polyadenylation signal |
| SV40 poly(A) signal 8811 .. 8945 = 135 bp SV40 polyadenylation signal |
| HIV-1 Ψ 681 .. 806 = 126 bp packaging signal of human immunodeficiency virus type 1 |
| HIV-1 Ψ 681 .. 806 = 126 bp packaging signal of human immunodeficiency virus type 1 |
| cPPT/CTS 2028 .. 2144 = 117 bp central polypurine tract and central termination sequence of HIV-1 (lacking the first T) |
| cPPT/CTS 2028 .. 2144 = 117 bp central polypurine tract and central termination sequence of HIV-1 (lacking the first T) |
| AmpR promoter 8659 .. 8763 = 105 bp |
| AmpR promoter 8659 .. 8763 = 105 bp |
| 3' MCS 3837 .. 3850 = 14 bp multiple cloning site downstream of DmrD |
| 3' MCS 3837 .. 3850 = 14 bp multiple cloning site downstream of DmrD |
| 5' MCS 2804 .. 2815 = 12 bp multiple cloning site upstream of DmrD |
| 5' MCS 2804 .. 2815 = 12 bp multiple cloning site upstream of DmrD |
| ORF: 5185 .. 5481 = 297 bp ORF: 98 amino acids = 10.2 kDa |
| ORF: 1181 .. 2032 = 852 bp ORF: 283 amino acids = 32.7 kDa |
| ORF: 2822 .. 3859 = 1038 bp ORF: 345 amino acids = 38.2 kDa |
| ORF: 4346 .. 5068 = 723 bp ORF: 240 amino acids = 26.2 kDa |
| ORF: 7928 .. 8194 = 267 bp ORF: 88 amino acids = 9.2 kDa |
| ORF: 5172 .. 5699 = 528 bp ORF: 175 amino acids = 19.0 kDa |
| ORF: 1015 .. 1476 = 462 bp ORF: 153 amino acids = 16.6 kDa |
| ORF: 7798 .. 8658 = 861 bp ORF: 286 amino acids = 31.6 kDa |
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