pRSIT17-U6Tet-sh-HTS6-CMV-TetRep-2A-TagGFP2-2A-Puro

Lentiviral vector for inducible barcoded shRNA libraries, with an HTS6 cassette for high-throughput sequencing plus TagGFP2 and puromycin resistance markers.

Sequence Author: Cellecta

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HomePlasmidsViral Expression & Packaging VectorspRSIT17-U6Tet-sh-HTS6-CMV-TetRep-2A-TagGFP2-2A-Puro
MluI (1) SspI (8089) ScaI (7765) PciI (6392) SfiI (5970) AanI (5741) AanI (5685) KpnI (5350) Acc65I (5346) HincII (4751) SalI (4749) SexAI * (4710) EagI (4591) SphI (96) NruI * (612) MfeI (964) BbvCI (1199) PpuMI - SanDI (1709) BspDI - ClaI (1798) BbsI - BpiI (2065) cloning site BbsI - BpiI (2091) PaeR7I - PspXI - XhoI (2114) EcoRI (2281) SnaBI (2362) XbaI * (2654) BstXI (2750) Bsu36I (2898) BlpI (3208) BclI * (3238) BsaBI * (3936) NheI (4065) BmtI (4069) PflFI - Tth111I (4185) BsiWI (4199) RsrII (4259) pRSIT17-U6Tet-sh-HTS6-CMV- TetRep-2A-TagGFP2-2A-Puro 8676 bp