pLP1

Lentiviral packaging plasmid containing the HIV-1 gag and pol genes.

Sequence Author: Thermo Fisher (Invitrogen)

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HomePlasmidsViral Expression & Packaging VectorspLP1
Bsu36I (8815) PvuI (8256) FspI (8108) PspFI (7301) BseYI (7297) HaeII (7241) SacII (6067) AleI (5960) BbvCI - Bpu10I (5807) BspEI (5672) BfuAI - BspMI - PaqCI (5611) AflII (5308) NdeI (328) SnaBI (434) BsmBI - Esp3I (683) PmlI (1323) BspDI * - ClaI * (1396) SphI (2012) MfeI (2533) AvrII (2576) BclI * (2994) SbfI (3408) BstZ17I (3494) EcoRV (3544) PflFI - Tth111I (3646) AgeI (4050) pLP1 8889 bp
Bsu36I  (8815)
1 site		 C C T N A G G G G A N T C C
Sticky ends from different Bsu36I sites may not be compatible.
PvuI  (8256)
1 site		 C G A T C G G C T A G C
FspI  (8108)
1 site		 T G C G C A A C G C G T
PspFI  (7301)
1 site		 C C C A G C G G G T C G
BseYI  (7297)
1 site		 C C C A G C G G G T C G
After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
HaeII  (7241)
1 site		 R G C G C Y Y C G C G R
SacII  (6067)
1 site		 C C G C G G G G C G C C
Efficient cleavage requires at least two copies of the SacII recognition sequence.
AleI  (5960)
1 site		 C A C N N N N G T G G T G N N N N C A C
BbvCI  (5807)
1 site		 C C T C A G C G G A G T C G
Bpu10I  (5807)
1 site		 C C T N A G C G G A N T C G
Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BspEI  (5672)
1 site		 T C C G G A A G G C C T
BfuAI  (5611)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (5611)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
PaqCI  (5611)
1 site		 C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the PaqCI recognition sequence.
Sticky ends from different PaqCI sites may not be compatible.
Cleavage can be improved with PaqCI Activator.
AflII  (5308)
1 site		 C T T A A G G A A T T C
NdeI  (328)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (434)
1 site		 T A C G T A A T G C A T
BsmBI  (683)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
Esp3I  (683)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different Esp3I sites may not be compatible.
PmlI  (1323)
1 site		 C A C G T G G T G C A C
BspDI  (1396)
1 site		 A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (1396)
1 site		 A T C G A T T A G C T A
* Blocked by Dam methylation.
SphI  (2012)
1 site		 G C A T G C C G T A C G
MfeI  (2533)
1 site		 C A A T T G G T T A A C
AvrII  (2576)
1 site		 C C T A G G G G A T C C
BclI  (2994)
1 site		 T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
SbfI  (3408)
1 site		 C C T G C A G G G G A C G T C C
BstZ17I  (3494)
1 site		 G T A T A C C A T A T G
EcoRV  (3544)
1 site		 G A T A T C C T A T A G
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
PflFI  (3646)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (3646)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
AgeI  (4050)
1 site		 A C C G G T T G G C C A
pol
2650 .. 5661  =  3012 bp
1003 amino acids  =  113.7 kDa
Product: HIV-1 Pol polyprotein
pol
2650 .. 5661  =  3012 bp
1003 amino acids  =  113.7 kDa
Product: HIV-1 Pol polyprotein
AmpR
7813 .. 8673  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   7813 .. 8604  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
7813 .. 8673  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   8605 .. 8673  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
7813 .. 8673  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
7054 .. 7642  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
7054 .. 7642  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
β-globin intron
796 .. 1271  =  476 bp
internally truncated intron from human β-globin
β-globin intron
796 .. 1271  =  476 bp
internally truncated intron from human β-globin
β-globin poly(A)
6150 .. 6544  =  395 bp
human β-globin polyadenylation signal
β-globin poly(A)
6150 .. 6544  =  395 bp
human β-globin polyadenylation signal
CMV enhancer
79 .. 458  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
79 .. 458  =  380 bp
human cytomegalovirus immediate early enhancer
RRE
5686 .. 5919  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
RRE
5686 .. 5919  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
CMV promoter
459 .. 662  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
459 .. 662  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
AmpR promoter
8674 .. 8778  =  105 bp
AmpR promoter
8674 .. 8778  =  105 bp
gag
1355 .. 2857  =  1503 bp
500 amino acids  =  55.9 kDa
Product: HIV-1 Gag polyprotein
gag
1355 .. 2857  =  1503 bp
500 amino acids  =  55.9 kDa
Product: HIV-1 Gag polyprotein
cPPT/CTS
5346 .. 5463  =  118 bp
central polypurine tract and central termination sequence of HIV-1
cPPT/CTS
5346 .. 5463  =  118 bp
central polypurine tract and central termination sequence of HIV-1
ORF:  2923 .. 5661  =  2739 bp
ORF:  912 amino acids  =  103.5 kDa
ORF:  1355 .. 2857  =  1503 bp
ORF:  500 amino acids  =  55.9 kDa
ORF:  5606 .. 6124  =  519 bp
ORF:  172 amino acids  =  19.3 kDa
ORF:  7943 .. 8209  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  5431 .. 5859  =  429 bp
ORF:  142 amino acids  =  15.9 kDa
ORF:  7813 .. 8673  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  4140 .. 4415  =  276 bp
ORF:  91 amino acids  =  10.0 kDa
ORF:  8570 .. 8830  =  261 bp
ORF:  86 amino acids  =  9.8 kDa
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Individual Sequences & Maps

pAAV-AC-GFP
pLenti6.2 N-Lumio V5-DEST
pLVX-rHom-1
pRetroX-Tet3G
pAAV-AC-Myc-DDK
pLenti6.2 V5-DEST
pLVX-rHom-Nuc1
pRetroX-TetOne
pAAV-AC-RFP
pLenti6.3 TO V5-DEST
pLVX-rHom-Sec1
pRetroX-TetOne-Puro
pAAV2-EF1a-tGFP-WPRE
pLenti6.3 V5-DEST
pLVX-Tet-Off Advanced
pRetroX-Tight-Hyg
pAd BLOCK-iT-DEST
pLenti6.4 R4R2 V5-DEST
pLVX-Tet-On Advanced
pRetroX-Tight-Pur
pAd CMV V5-DEST
pLenti6 BLOCK-iT-DEST
pLVX-Tet3G
pRetroX-TRE3G
pAd PL-DEST
pLenti6 capTEV-CT-DEST
pLVX-TetOne
pRFP-C-RS
pAdenoX-CMV Linear
pLenti6 capTEV-NT-DEST1
pLVX-TetOne-Puro
pRFP-CB-shLenti
pAdenoX-DsRed-Express Linear
pLenti6 TR
pLVX-Tight-Puro
pRS
pAdenoX-PRLS Linear
pLenti6 UbC V5-DEST
pLVX-TRE3G
pRSGC1-U6-sg-HTS6-CMV-Cas9-2A-Puro
pAdenoX-PRLS-DsRed-Express Linear
pLenti6 V5-DEST
pLVX-TRE3G-Hom1
pRSGC2-U6-sg-HTS6-UbiC-Cas9-2A-Puro
pAdenoX-PRLS-ZsGreen1 Linear
pLenti7.3 V5-DEST
pLVX-TRE3G-IRES
pRSI12-U6-sh-HTS4-UbiC-TagRFP-2A-Puro
pAdenoX-Tet3G Linear
pLEX-MCS
pLVX-TRE3G-mCherry
pRSI16-U6-sh-HTS6-UbiC-TagRFP-2A-Puro
pAdenoX-ZsGreen1 Linear
pLHCX
pLVX-TRE3G-ZsGreen1
pRSI16cb-U6-sh-13kCB18-HTS6-UbiC-TagRFP-2A-Puro
pB-RS
pLKO.1
pLX301
pRSI17-U6-sh-HTS6-UbiC-TagGFP2-2A-Puro
pBABE-Hygro
pLKO.1 puro
pLX302
pRSI17cb-U6-sh-13kCB18-HTS6-UbiC-TagGFP2-2A-Puro
pBABE-Neo
pLMN-ZsGreen-Neomycin
pLX303
pRSIT12-U6Tet-sh-HTS4-CMV-TetRep-2A-TagRFP-2A-Puro
pBABE-Puro
pLMPd-Ametrine
pLX304
pRSIT16-U6Tet-sh-HTS6-CMV-TetRep-2A-TagRFP-2A-Puro
pBABE-Zeo
pLNCX2
pLX304-V5-Blast-Empty
pRSIT17-U6Tet-sh-HTS6-CMV-TetRep-2A-TagGFP2-2A-Puro
pCMV-VSV-G
pLP VSVG
pLXIN
pRSITEP-U6Tet-sh-noHTS-EF1-TetRep-2A-Puro
pGFP-B-RS
pLP1
pLXSN
pRSITUR-U6Tet-sh-HTS3-UbiC-TetRep-2A-TagRFP
pGFP-C-shLenti
pLP2
pMD2.G
pShuttle2
pGFP-V-RS
pLPCX
pMSCV16URP-U6-sh-HTS6-UbiC-TagRFP-2A-Puro
pSIREN-RetroQ
pGIPZ
pLVX-EF1alpha-IRES-mCherry
pMSCVhyg
pSIREN-RetroQ-DsRed-Express
pLenti-C-GFP
pLVX-EF1alpha-IRES-Puro
pMSCVneo
pSIREN-RetroQ-TetH
pLenti-C-mGFP
pLVX-EF1alpha-IRES-ZsGreen1
pMSCVpuro
pSIREN-RetroQ-TetP
pLenti-C-Myc-DDK
pLVX-EF1alpha-Tet3G
pQC-tTS-IN
pSIREN-RetroQ-ZsGreen1
pLenti-C-Myc-DDK-IRES-BSD
pLVX-Het-1
pQCXIH
psPAX2
pLenti-C-Myc-DDK-IRES-GFP
pLVX-Het-2
pQCXIN
pTRE3G-Hom1
pLenti-C-Myc-DDK-IRES-Neo
pLVX-Het-Mem1
pQCXIP
pTRIPZ
pLenti-C-Myc-DDK-IRES-Puro
pLVX-Het-Nuc1
pQCXIX
ptTS-Neo
pLenti-C-Myc-DDK-IRES-tRFP
pLVX-Hom-1
pRetro-Lib
pZIP-hCMV-RFP-Puro
pLenti-C-tRFP
pLVX-Hom-Mem1
pRetroQ-AcGFP1-C1
pZIP-hCMV-ZSGreen-Puro
pLenti-C-tYFP
pLVX-Hom-Nuc1
pRetroQ-AcGFP1-N1
pZIP-hEF1-alpha-RFP-Puro
pLenti-EF1a-C-mGFP
pLVX-IRES-Hyg
pRetroQ-DsRed-Monomer-C1
pZIP-hEF1-alpha-ZsGreen-Puro
pLenti-EF1a-C-Myc-DDK-IRES-Puro
pLVX-IRES-mCherry
pRetroQ-DsRed-Monomer-N1
pZIP-mCMV-RFP-Puro
pLenti-EF1a-C-tGFP
pLVX-IRES-Neo
pRetroQ-mCherry-C1
pZIP-mCMV-ZsGreen-Puro
pLenti-N-tGFP
pLVX-IRES-Puro
pRetroQ-mCherry-N1
pZIP-mEF1-alpha-RFP-Puro
pLenti-N-tRFP
pLVX-IRES-tdTomato
pRetroX-IRES-DsRedExpress
pZIP-mEF1-alpha-ZsGreen-Puro
pLenti-N-tYFP
pLVX-IRES-ZsGreen1
pRetroX-IRES-ZsGreen1
pZIP-SFFV-RFP-Puro
pLenti3.3 TR
pLVX-PTuner
pRetroX-PTuner
pZIP-SFFV-ZsGreen-Puro
pLenti4 BLOCK-iT-DEST
pLVX-PTuner Green
pRetroX-PTuner IRES
pZIP-TRE3G-ZsGreen-Puro
pLenti4 V5-DEST
pLVX-PTunerC
pRetroX-Tet-Off Advanced
pSIREN-Shuttle (linearized)
pLenti6.2 C-Lumio V5-DEST
pLVX-Puro
pRetroX-Tet-On Advanced
scramble shRNA

Plasmid Sets

Basic Cloning Vectors
I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

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