pRSITUR-U6Tet-sh-HTS3-UbiC-TetRep-2A-TagRFP

Lentiviral vector for inducible barcoded shRNA libraries, with an HTS3 cassette for high-throughput sequencing plus a TagRFP marker.

Sequence Author: Cellecta

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HomePlasmidsViral Expression & Packaging VectorspRSITUR-U6Tet-sh-HTS3-UbiC-TetRep-2A-TagRFP
MluI (1) SspI (7517) ScaI (7193) PciI (5820) SfiI (5398) NcoI (5352) AanI (5169) AanI (5113) KpnI (4778) Acc65I (4774) NaeI (4697) NgoMIV (4695) HincII (4179) SalI (4177) SphI (96) BssHII (490) NruI * (612) MfeI (964) BbvCI (1199) AleI (1352) PpuMI - SanDI (1709) BspDI - ClaI (1798) BsaAI (1928) BbsI - BpiI (2065) cloning site BbsI - BpiI (2091) EcoRI (2154) PaeR7I - XhoI (2165) BstBI (2356) AfeI (2416) XbaI (2760) BstXI (2856) BlpI (3314) BclI * (3344) BamHI (3391) BspEI (3451) BsrGI (3506) PasI (3687) PshAI (4096) pRSITUR-U6Tet-sh-HTS3-UbiC-TetRep-2A-TagRFP 8104 bp
MluI  (1)
1 site		 A C G C G T T G C G C A
SspI  (7517)
1 site		 A A T A T T T T A T A A
ScaI  (7193)
1 site		 A G T A C T T C A T G A
PciI  (5820)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
SfiI  (5398)
1 site		 G G C C N N N N N G G C C C C G G N N N N N C C G G
Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
NcoI  (5352)
1 site		 C C A T G G G G T A C C
AanI  (5169)
2 sites		 T T A T A A A A T A T T
AanI  (5113)
2 sites		 T T A T A A A A T A T T
KpnI  (4778)
1 site		 G G T A C C C C A T G G
Acc65I  (4774)
1 site		 G G T A C C C C A T G G
NaeI  (4697)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (4695)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
HincII  (4179)
1 site		 G T Y R A C C A R Y T G
SalI  (4177)
1 site		 G T C G A C C A G C T G
SphI  (96)
1 site		 G C A T G C C G T A C G
BssHII  (490)
1 site		 G C G C G C C G C G C G
BssHII is typically used at 50°C, but is 75% active at 37°C.
NruI  (612)
1 site		 T C G C G A A G C G C T
* Blocked by Dam methylation.
MfeI  (964)
1 site		 C A A T T G G T T A A C
BbvCI  (1199)
1 site		 C C T C A G C G G A G T C G
AleI  (1352)
1 site		 C A C N N N N G T G G T G N N N N C A C
PpuMI  (1709)
1 site		 R G G W C C Y Y C C W G G R
Sticky ends from different PpuMI sites may not be compatible.
SanDI  (1709)
1 site		 G G G W C C C C C C W G G G
Sticky ends from different SanDI sites may not be compatible.
BspDI  (1798)
1 site		 A T C G A T T A G C T A
ClaI  (1798)
1 site		 A T C G A T T A G C T A
BsaAI  (1928)
1 site		 Y A C G T R