pAdenoX-Tet3G Linear

In-Fusion®-ready linearized adenoviral vector for doxycycline-inducible expression of a gene.

Sequence Author: Clontech (TaKaRa)

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End (36,043) In-Fusion Cloning site Ad5 Ψ Adeno-X fwd screening primer (35,368 .. 35,392) M13 rev AmpR promoter M13 fwd BstBI (32,297) SV40 poly(A) signal CMV promoter I-PpoI (25,302) SrfI (24,953) MreI (23,818) AsiSI (18,871) Start (0) In-Fusion Cloning site stop codons small t intron Adeno-X rev screening primer (936 .. 955) BstZ17I (3197) PmeI (10,687) pAdenoX-Tet3G Linear 36,043 bp
End  (36,043)
0 sites
BstBI  (32,297)
1 site
T T C G A A A A G C T T
I-PpoI  (25,302)
1 site
C T C T C T T A A G G T A G C G A G A G A A T T C C A T C G

I-PpoI is a homing endonuclease that can recognize a variety of similar recognition sequences.
SrfI  (24,953)
1 site
G C C C G G G C C G G G C C C G
MreI  (23,818)
1 site
C G C C G G C G G C G G C C G C
AsiSI  (18,871)
1 site
G C G A T C G C C G C T A G C G
Start  (0)
0 sites
BstZ17I  (3197)
1 site
G T A T A C C A T A T G
PmeI  (10,687)
1 site
G T T T A A A C C A A A T T T G
Adeno-X fwd screening primer
25-mer  /  52% GC
1 binding site
35,368 .. 35,392  =  25 annealed bases
Tm  =  64°C
Adeno-X rev screening primer
20-mer  /  50% GC
1 binding site
936 .. 955  =  20 annealed bases
Tm  =  56°C
adenovirus DNA
956 .. 25,286  =  24,331 bp
adenovirus DNA
956 .. 25,286  =  24,331 bp
adenovirus DNA
27,337 .. 32,172  =  4836 bp
adenovirus DNA
27,337 .. 32,172  =  4836 bp
AmpR
33,176 .. 34,036  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   33,176 .. 33,244  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
33,176 .. 34,036  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   33,245 .. 34,036  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
33,176 .. 34,036  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
Tet-On 3G
26,097 .. 26,843  =  747 bp
248 amino acids  =  27.7 kDa
Product: modified rtTA protein that binds tightly to promoters containing the tet operator in the presence of doxycycline
Tet-On 3G
26,097 .. 26,843  =  747 bp
248 amino acids  =  27.7 kDa
Product: modified rtTA protein that binds tightly to promoters containing the tet operator in the presence of doxycycline
ori
34,207 .. 34,795  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
34,207 .. 34,795  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
32,537 .. 32,992  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
32,537 .. 32,992  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
CMV enhancer
25,411 .. 25,790  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
25,411 .. 25,790  =  380 bp
human cytomegalovirus immediate early enhancer
TRE3G promoter
35,645 .. 36,020  =  376 bp
3rd-generation Tet-responsive promoter that can be activated by binding of Tet-On 3G
TRE3G promoter
35,645 .. 36,020  =  376 bp
3rd-generation Tet-responsive promoter that can be activated by binding of Tet-On 3G
CMV promoter
25,791 .. 25,994  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
25,791 .. 25,994  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
Ad5 Ψ
35,455 .. 35,605  =  151 bp
packaging signal for adenovirus serotype 5
Ad5 Ψ
35,455 .. 35,605  =  151 bp
packaging signal for adenovirus serotype 5
SV40 poly(A) signal
26,853 .. 26,987  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
26,853 .. 26,987  =  135 bp
SV40 polyadenylation signal
AmpR promoter
33,071 .. 33,175  =  105 bp
AmpR promoter
33,071 .. 33,175  =  105 bp
ITR
32,173 .. 32,275  =  103 bp
inverted terminal repeat of human adenovirus serotype 5
ITR
32,173 .. 32,275  =  103 bp
inverted terminal repeat of human adenovirus serotype 5
ITR
35,265 .. 35,367  =  103 bp
inverted terminal repeat of human adenovirus serotype 5
ITR
35,265 .. 35,367  =  103 bp
inverted terminal repeat of human adenovirus serotype 5
SV40 poly(A) signal
822 .. 903  =  82 bp
SV40 polyadenylation signal
SV40 poly(A) signal
822 .. 903  =  82 bp
SV40 polyadenylation signal
small t intron
182 .. 247  =  66 bp
simian virus 40 (SV40) small t antigen intron
small t intron
182 .. 247  =  66 bp
simian virus 40 (SV40) small t antigen intron
M13 fwd
32,380 .. 32,396  =  17 bp
common sequencing primer, one of multiple similar variants
M13 fwd
32,380 .. 32,396  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
35,181 .. 35,197  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
35,181 .. 35,197  =  17 bp
common sequencing primer, one of multiple similar variants
In-Fusion Cloning site
1 .. 15  =  15 bp
sequence to be added to 5' end of reverse primer
In-Fusion Cloning site
1 .. 15  =  15 bp
sequence to be added to 5' end of reverse primer
In-Fusion Cloning site
36,029 .. 36,043  =  15 bp
sequence to be added to 5' end of forward primer
In-Fusion Cloning site
36,029 .. 36,043  =  15 bp
sequence to be added to 5' end of forward primer
stop codons
35 .. 45  =  11 bp
stop codons in all three reading frames
stop codons
35 .. 45  =  11 bp
stop codons in all three reading frames
tet operator
35,650 .. 35,668  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
35,650 .. 35,668  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
35,686 .. 35,704  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
35,686 .. 35,704  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
35,722 .. 35,740  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
35,722 .. 35,740  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
35,758 .. 35,776  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
35,758 .. 35,776  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
35,794 .. 35,812  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
35,794 .. 35,812  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
35,830 .. 35,848  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
35,830 .. 35,848  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
35,866 .. 35,884  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
35,866 .. 35,884  =  19 bp
bacterial operator O2 for the tetR and tetA genes
ORF:  805 .. 1059  =  255 bp
ORF:  84 amino acids  =  9.9 kDa
ORF:  3721 .. 4041  =  321 bp
ORF:  106 amino acids  =  11.7 kDa
ORF:  4741 .. 4992  =  252 bp
ORF:  83 amino acids  =  9.4 kDa
ORF:  5926 .. 6162  =  237 bp
ORF:  78 amino acids  =  7.9 kDa
ORF:  8479 .. 9726  =  1248 bp
ORF:  415 amino acids  =  47.0 kDa
ORF:  11,413 .. 11,643  =  231 bp
ORF:  76 amino acids  =  8.7 kDa
ORF:  14,716 .. 15,195  =  480 bp
ORF:  159 amino acids  =  17.4 kDa
ORF:  15,433 .. 16,185  =  753 bp
ORF:  250 amino acids  =  27.0 kDa
ORF:  19,162 .. 19,776  =  615 bp
ORF:  204 amino acids  =  23.1 kDa
ORF:  20,908 .. 23,913  =  3006 bp
ORF:  1001 amino acids  =  110.0 kDa
ORF:  26,254 .. 26,556  =  303 bp
ORF:  100 amino acids  =  11.1 kDa
ORF:  27,367 .. 29,124  =  1758 bp
ORF:  585 amino acids  =  62.1 kDa
ORF:  326 .. 799  =  474 bp
ORF:  157 amino acids  =  18.2 kDa
ORF:  1040 .. 1462  =  423 bp
ORF:  140 amino acids  =  14.5 kDa
ORF:  7862 .. 8242  =  381 bp
ORF:  126 amino acids  =  13.5 kDa
ORF:  16,271 .. 19,129  =  2859 bp
ORF:  952 amino acids  =  107.9 kDa
ORF:  21,164 .. 21,520  =  357 bp
ORF:  118 amino acids  =  12.8 kDa
ORF:  21,863 .. 22,249  =  387 bp
ORF:  128 amino acids  =  14.3 kDa
ORF:  23,426 .. 24,208  =  783 bp
ORF:  260 amino acids  =  28.8 kDa
ORF:  29,072 .. 29,470  =  399 bp
ORF:  132 amino acids  =  15.0 kDa
ORF:  30,293 .. 30,790  =  498 bp
ORF:  165 amino acids  =  18.7 kDa
ORF:  33,176 .. 34,036  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  5409 .. 5858  =  450 bp
ORF:  149 amino acids  =  16.7 kDa
ORF:  6735 .. 7241  =  507 bp
ORF:  168 amino acids  =  17.7 kDa
ORF:  9747 .. 11,504  =  1758 bp
ORF:  585 amino acids  =  65.3 kDa
ORF:  11,586 .. 13,301  =  1716 bp
ORF:  571 amino acids  =  63.3 kDa
ORF:  13,308 .. 13,904  =  597 bp
ORF:  198 amino acids  =  22.0 kDa
ORF:  13,941 .. 15,080  =  1140 bp
ORF:  379 amino acids  =  42.7 kDa
ORF:  15,108 .. 15,350  =  243 bp
ORF:  80 amino acids  =  8.8 kDa
ORF:  24,597 .. 25,280  =  684 bp
ORF:  227 amino acids  =  24.7 kDa
ORF:  25,950 .. 26,843  =  894 bp
ORF:  297 amino acids  =  33.8 kDa
ORF:  27,078 .. 27,332  =  255 bp
ORF:  84 amino acids  =  9.0 kDa
ORF:  30,804 .. 31,142  =  339 bp
ORF:  112 amino acids  =  12.5 kDa
ORF:  35,673 .. 35,939  =  267 bp
ORF:  88 amino acids  =  10.5 kDa
ORF:  2768 .. 3115  =  348 bp
ORF:  115 amino acids  =  12.2 kDa
ORF:  6014 .. 7975  =  1962 bp
ORF:  653 amino acids  =  74.7 kDa
ORF:  8183 .. 8548  =  366 bp
ORF:  121 amino acids  =  12.7 kDa
ORF:  9743 .. 10,003  =  261 bp
ORF:  86 amino acids  =  9.0 kDa
ORF:  14,627 .. 14,878  =  252 bp
ORF:  83 amino acids  =  8.3 kDa
ORF:  15,077 .. 15,367  =  291 bp
ORF:  96 amino acids  =  10.9 kDa
ORF:  26,549 .. 27,004  =  456 bp
ORF:  151 amino acids  =  16.0 kDa
ORF:  30,335 .. 30,679  =  345 bp
ORF:  114 amino acids  =  13.3 kDa
ORF:  31,037 .. 31,447  =  411 bp
ORF:  136 amino acids  =  15.3 kDa
ORF:  1522 .. 2880  =  1359 bp
ORF:  452 amino acids  =  51.1 kDa
ORF:  3883 .. 4221  =  339 bp
ORF:  112 amino acids  =  12.8 kDa
ORF:  5893 .. 6471  =  579 bp
ORF:  192 amino acids  =  20.8 kDa
ORF:  8689 .. 9057  =  369 bp
ORF:  122 amino acids  =  13.6 kDa
ORF:  16,303 .. 16,578  =  276 bp
ORF:  91 amino acids  =  10.2 kDa
ORF:  29,530 .. 30,414  =  885 bp
ORF:  294 amino acids  =  34.1 kDa
ORF:  31,477 .. 31,863  =  387 bp
ORF:  128 amino acids  =  14.3 kDa
ORF:  33,640 .. 33,906  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  1905 .. 2129  =  225 bp
ORF:  74 amino acids  =  8.3 kDa
ORF:  2628 .. 5798  =  3171 bp
ORF:  1056 amino acids  =  120.4 kDa
ORF:  7731 .. 8144  =  414 bp
ORF:  137 amino acids  =  15.3 kDa
ORF:  8988 .. 9281  =  294 bp
ORF:  97 amino acids  =  10.5 kDa
ORF:  11,292 .. 11,549  =  258 bp
ORF:  85 amino acids  =  9.5 kDa
ORF:  11,811 .. 12,596  =  786 bp
ORF:  261 amino acids  =  28.5 kDa
ORF:  14,073 .. 14,342  =  270 bp
ORF:  89 amino acids  =  9.5 kDa
ORF:  15,591 .. 16,223  =  633 bp
ORF:  210 amino acids  =  22.5 kDa
ORF:  19,872 .. 21,461  =  1590 bp
ORF:  529 amino acids  =  59.1 kDa
ORF:  23,856 .. 24,146  =  291 bp
ORF:  96 amino acids  =  10.5 kDa
ORF:  26,952 .. 27,368  =  417 bp
ORF:  138 amino acids  =  15.1 kDa
ORF:  27,495 .. 27,965  =  471 bp
ORF:  156 amino acids  =  16.6 kDa
ORF:  28,299 .. 28,544  =  246 bp
ORF:  81 amino acids  =  8.7 kDa
ORF:  30,690 .. 31,040  =  351 bp
ORF:  116 amino acids  =  13.3 kDa
ORF:  32,775 .. 33,020  =  246 bp
ORF:  81 amino acids  =  8.6 kDa
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