pZIP-TRE3G-ZsGreen-Puro

Lentiviral vector with a doxycycline-inducible cassette encoding ZsGreen plus a short hairpin RNA (shRNA) with an "UltramiR" microRNA scaffold.

Sequence Author: transOMIC

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HomePlasmidsViral Expression & Packaging VectorspZIP-TRE3G-ZsGreen-Puro
cPPT/CTS shRNA 5' miR-30a pZIP-TRE3G-ZsGreen-Puro 10,321 bp
AmpR
8959 .. 9819  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   8959 .. 9750  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
8959 .. 9819  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   9751 .. 9819  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
8959 .. 9819  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
Tet-On® 3G
4531 .. 5277  =  747 bp
248 amino acids  =  27.7 kDa
Product: modified rtTA protein that binds tightly to promoters containing the tet operator in the presence of doxycycline
Tet-On® 3G
4531 .. 5277  =  747 bp
248 amino acids  =  27.7 kDa
Product: modified rtTA protein that binds tightly to promoters containing the tet operator in the presence of doxycycline
ZsGreen1
2879 .. 3574  =  696 bp
231 amino acids  =  26.1 kDa
Product: Zoanthus green fluorescent protein
mammalian codon-optimized
ZsGreen1
2879 .. 3574  =  696 bp
231 amino acids  =  26.1 kDa
Product: Zoanthus green fluorescent protein
mammalian codon-optimized
LTR
1 .. 634  =  634 bp
long terminal repeat (LTR) from HIV-1
LTR
1 .. 634  =  634 bp
long terminal repeat (LTR) from HIV-1
LTR
7039 .. 7672  =  634 bp
long terminal repeat (LTR) from HIV-1
LTR
7039 .. 7672  =  634 bp
long terminal repeat (LTR) from HIV-1
PuroR
5626 .. 6225  =  600 bp
199 amino acids  =  21.5 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
PuroR
5626 .. 6225  =  600 bp
199 amino acids  =  21.5 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
WPRE
6243 .. 6831  =  589 bp
woodchuck hepatitis virus posttranscriptional regulatory element
WPRE
6243 .. 6831  =  589 bp
woodchuck hepatitis virus posttranscriptional regulatory element
ori
8203 .. 8788  =  586 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
8203 .. 8788  =  586 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
hPGK promoter
4002 .. 4512  =  511 bp
human phosphoglycerate kinase 1 promoter
hPGK promoter
4002 .. 4512  =  511 bp
human phosphoglycerate kinase 1 promoter
TRE3GS promoter
3614 .. 3981  =  368 bp
3rd-generation Tet-responsive promoter that can be activated by binding of Tet-On® 3G, modified to eliminate binding sites for endogenous mammalian transcription factors
TRE3GS promoter
3614 .. 3981  =  368 bp
3rd-generation Tet-responsive promoter that can be activated by binding of Tet-On® 3G, modified to eliminate binding sites for endogenous mammalian transcription factors
SV40 promoter
5288 .. 5617  =  330 bp
SV40 enhancer and early promoter
SV40 promoter
5288 .. 5617  =  330 bp
SV40 enhancer and early promoter
RRE
1303 .. 1536  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
RRE
1303 .. 1536  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
SV40 poly(A) signal
2187 .. 2321  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
2187 .. 2321  =  135 bp
SV40 polyadenylation signal
3' miR-30a
2487 .. 2613  =  127 bp
sequence downstream of the 71-nt precursor of the human miR-30a microRNA (Zeng et al., 2002)
3' miR-30a
2487 .. 2613  =  127 bp
sequence downstream of the 71-nt precursor of the human miR-30a microRNA (Zeng et al., 2002)
HIV-1 Ψ
681 .. 806  =  126 bp
packaging signal of human immunodeficiency virus type 1
HIV-1 Ψ
681 .. 806  =  126 bp
packaging signal of human immunodeficiency virus type 1
5' miR-30a
2683 .. 2804  =  122 bp
sequence upstream of the 71-nt precursor of the human miR-30a microRNA (Zeng et al., 2002)
5' miR-30a
2683 .. 2804  =  122 bp
sequence upstream of the 71-nt precursor of the human miR-30a microRNA (Zeng et al., 2002)
cPPT/CTS
2028 .. 2143  =  116 bp
central polypurine tract and central termination sequence of HIV-1 (lacking the first T)
cPPT/CTS
2028 .. 2143  =  116 bp
central polypurine tract and central termination sequence of HIV-1 (lacking the first T)
AmpR promoter
9820 .. 9924  =  105 bp
AmpR promoter
9820 .. 9924  =  105 bp
shRNA
2614 .. 2682  =  69 bp
3 segments
   Segment 3:  
   2614 .. 2640  =  27 bp
short hairpin RNA
shRNA
2614 .. 2682  =  69 bp
3 segments
   Segment 2:  miR-30a loop  
   2641 .. 2655  =  15 bp
short hairpin RNA
shRNA
2614 .. 2682  =  69 bp
3 segments
   Segment 1:  
   2656 .. 2682  =  27 bp
short hairpin RNA
shRNA
2614 .. 2682  =  69 bp
3 segments
short hairpin RNA
SV40 ori
5468 .. 5603  =  136 bp
SV40 origin of replication
SV40 ori
5468 .. 5603  =  136 bp
SV40 origin of replication
tet operator
3739 .. 3757  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
3739 .. 3757  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
3775 .. 3793  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
3775 .. 3793  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
3811 .. 3829  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
3811 .. 3829  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
3847 .. 3865  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
3847 .. 3865  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
3883 .. 3901  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
3883 .. 3901  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
3919 .. 3937  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
3919 .. 3937  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
3955 .. 3973  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
3955 .. 3973  =  19 bp
bacterial operator O2 for the tetR and tetA genes
ORF:  4294 .. 5277  =  984 bp
ORF:  327 amino acids  =  36.0 kDa
ORF:  5626 .. 6225  =  600 bp
ORF:  199 amino acids  =  21.5 kDa
ORF:  6346 .. 6642  =  297 bp
ORF:  98 amino acids  =  10.2 kDa
ORF:  1181 .. 2032  =  852 bp
ORF:  283 amino acids  =  32.7 kDa
ORF:  4688 .. 4990  =  303 bp
ORF:  100 amino acids  =  11.1 kDa
ORF:  9089 .. 9355  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  2904 .. 3197  =  294 bp
ORF:  97 amino acids  =  10.9 kDa
ORF:  6333 .. 6860  =  528 bp
ORF:  175 amino acids  =  19.0 kDa
ORF:  2879 .. 3574  =  696 bp
ORF:  231 amino acids  =  26.1 kDa
ORF:  1015 .. 1476  =  462 bp
ORF:  153 amino acids  =  16.6 kDa
ORF:  8959 .. 9819  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  3681 .. 3950  =  270 bp
ORF:  89 amino acids  =  10.5 kDa
ORF:  4983 .. 5264  =  282 bp
ORF:  93 amino acids  =  9.6 kDa
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Individual Sequences & Maps

pAAV-AC-GFP
pLenti6.2 N-Lumio V5-DEST
pLVX-rHom-1
pRetroX-Tet3G
pAAV-AC-Myc-DDK
pLenti6.2 V5-DEST
pLVX-rHom-Nuc1
pRetroX-TetOne
pAAV-AC-RFP
pLenti6.3 TO V5-DEST
pLVX-rHom-Sec1
pRetroX-TetOne-Puro
pAAV2-EF1a-tGFP-WPRE
pLenti6.3 V5-DEST
pLVX-Tet-Off Advanced
pRetroX-Tight-Hyg
pAd BLOCK-iT-DEST
pLenti6.4 R4R2 V5-DEST
pLVX-Tet-On Advanced
pRetroX-Tight-Pur
pAd CMV V5-DEST
pLenti6 BLOCK-iT-DEST
pLVX-Tet3G
pRetroX-TRE3G
pAd PL-DEST
pLenti6 capTEV-CT-DEST
pLVX-TetOne
pRFP-C-RS
pAdenoX-CMV Linear
pLenti6 capTEV-NT-DEST1
pLVX-TetOne-Puro
pRFP-CB-shLenti
pAdenoX-DsRed-Express Linear
pLenti6 TR
pLVX-Tight-Puro
pRS
pAdenoX-PRLS Linear
pLenti6 UbC V5-DEST
pLVX-TRE3G
pRSGC1-U6-sg-HTS6-CMV-Cas9-2A-Puro
pAdenoX-PRLS-DsRed-Express Linear
pLenti6 V5-DEST
pLVX-TRE3G-Hom1
pRSGC2-U6-sg-HTS6-UbiC-Cas9-2A-Puro
pAdenoX-PRLS-ZsGreen1 Linear
pLenti7.3 V5-DEST
pLVX-TRE3G-IRES
pRSI12-U6-sh-HTS4-UbiC-TagRFP-2A-Puro
pAdenoX-Tet3G Linear
pLEX-MCS
pLVX-TRE3G-mCherry
pRSI16-U6-sh-HTS6-UbiC-TagRFP-2A-Puro
pAdenoX-ZsGreen1 Linear
pLHCX
pLVX-TRE3G-ZsGreen1
pRSI16cb-U6-sh-13kCB18-HTS6-UbiC-TagRFP-2A-Puro
pB-RS
pLKO.1
pLX301
pRSI17-U6-sh-HTS6-UbiC-TagGFP2-2A-Puro
pBABE-Hygro
pLKO.1 puro
pLX302
pRSI17cb-U6-sh-13kCB18-HTS6-UbiC-TagGFP2-2A-Puro
pBABE-Neo
pLMN-ZsGreen-Neomycin
pLX303
pRSIT12-U6Tet-sh-HTS4-CMV-TetRep-2A-TagRFP-2A-Puro
pBABE-Puro
pLMPd-Ametrine
pLX304
pRSIT16-U6Tet-sh-HTS6-CMV-TetRep-2A-TagRFP-2A-Puro
pBABE-Zeo
pLNCX2
pLX304-V5-Blast-Empty
pRSIT17-U6Tet-sh-HTS6-CMV-TetRep-2A-TagGFP2-2A-Puro
pCMV-VSV-G
pLP VSVG
pLXIN
pRSITEP-U6Tet-sh-noHTS-EF1-TetRep-2A-Puro
pGFP-B-RS
pLP1
pLXSN
pRSITUR-U6Tet-sh-HTS3-UbiC-TetRep-2A-TagRFP
pGFP-C-shLenti
pLP2
pMD2.G
pShuttle2
pGFP-V-RS
pLPCX
pMSCV16URP-U6-sh-HTS6-UbiC-TagRFP-2A-Puro
pSIREN-RetroQ
pGIPZ
pLVX-EF1alpha-IRES-mCherry
pMSCVhyg
pSIREN-RetroQ-DsRed-Express
pLenti-C-GFP
pLVX-EF1alpha-IRES-Puro
pMSCVneo
pSIREN-RetroQ-TetH
pLenti-C-mGFP
pLVX-EF1alpha-IRES-ZsGreen1
pMSCVpuro
pSIREN-RetroQ-TetP
pLenti-C-Myc-DDK
pLVX-EF1alpha-Tet3G
pQC-tTS-IN
pSIREN-RetroQ-ZsGreen1
pLenti-C-Myc-DDK-IRES-BSD
pLVX-Het-1
pQCXIH
psPAX2
pLenti-C-Myc-DDK-IRES-GFP
pLVX-Het-2
pQCXIN
pTRE3G-Hom1
pLenti-C-Myc-DDK-IRES-Neo
pLVX-Het-Mem1
pQCXIP
pTRIPZ
pLenti-C-Myc-DDK-IRES-Puro
pLVX-Het-Nuc1
pQCXIX
ptTS-Neo
pLenti-C-Myc-DDK-IRES-tRFP
pLVX-Hom-1
pRetro-Lib
pZIP-hCMV-RFP-Puro
pLenti-C-tRFP
pLVX-Hom-Mem1
pRetroQ-AcGFP1-C1
pZIP-hCMV-ZSGreen-Puro
pLenti-C-tYFP
pLVX-Hom-Nuc1
pRetroQ-AcGFP1-N1
pZIP-hEF1-alpha-RFP-Puro
pLenti-EF1a-C-mGFP
pLVX-IRES-Hyg
pRetroQ-DsRed-Monomer-C1
pZIP-hEF1-alpha-ZsGreen-Puro
pLenti-EF1a-C-Myc-DDK-IRES-Puro
pLVX-IRES-mCherry
pRetroQ-DsRed-Monomer-N1
pZIP-mCMV-RFP-Puro
pLenti-EF1a-C-tGFP
pLVX-IRES-Neo
pRetroQ-mCherry-C1
pZIP-mCMV-ZsGreen-Puro
pLenti-N-tGFP
pLVX-IRES-Puro
pRetroQ-mCherry-N1
pZIP-mEF1-alpha-RFP-Puro
pLenti-N-tRFP
pLVX-IRES-tdTomato
pRetroX-IRES-DsRedExpress
pZIP-mEF1-alpha-ZsGreen-Puro
pLenti-N-tYFP
pLVX-IRES-ZsGreen1
pRetroX-IRES-ZsGreen1
pZIP-SFFV-RFP-Puro
pLenti3.3 TR
pLVX-PTuner
pRetroX-PTuner
pZIP-SFFV-ZsGreen-Puro
pLenti4 BLOCK-iT-DEST
pLVX-PTuner Green
pRetroX-PTuner IRES
pZIP-TRE3G-ZsGreen-Puro
pLenti4 V5-DEST
pLVX-PTunerC
pRetroX-Tet-Off Advanced
pSIREN-Shuttle (linearized)
pLenti6.2 C-Lumio V5-DEST
pLVX-Puro
pRetroX-Tet-On Advanced
scramble shRNA

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