pTRE3G-Hom1
Mammalian vector encoding a doxycycline-inducible FKBP homodimerizer domain, for creating soluble fusion proteins that can be dimerized with a drug.
Sequence Author: Clontech (TaKaRa)
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Efficient cleavage requires at least two copies of the NmeAIII recognition sequence. Sticky ends from different NmeAIII sites may not be compatible.For full activity, add fresh S-adenosylmethionine (SAM). |
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Sticky ends from different BglI sites may not be compatible. |
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Cleavage may be enhanced when more than one copy of the BsrFI recognition sequence is present. After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility. |
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Sticky ends from different BsaI sites may not be compatible.BsaI can be used between 37°C and 50°C. |
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The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible. |
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Sticky ends from different AlwNI sites may not be compatible. |
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Sticky ends from different DrdI sites may not be compatible. |
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PaeR7I does not recognize the sequence CTCTCGAG. |
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Efficient cleavage requires at least two copies of the NarI recognition sequence. |
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Efficient cleavage requires at least two copies of the PluTI recognition sequence. |
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Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.Sticky ends from different AccI sites may not be compatible. |
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ApaI can be used between 25°C and 37°C. |
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| * Blocked by Dam methylation. |
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| * Blocked by Dam methylation. |
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EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage. |
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Efficient cleavage requires at least two copies of the PaqCI recognition sequence. Sticky ends from different PaqCI sites may not be compatible.Cleavage can be improved with PaqCI Activator. |
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Sticky ends from different BsmBI sites may not be compatible.BsmBI-v2 is an improved version of BsmBI. |
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Sticky ends from different Esp3I sites may not be compatible. |
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Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present. |
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SmaI can be used at 37°C for brief incubations. |
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Prolonged incubation with NdeI may lead to removal of additional nucleotides. |
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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Sticky ends from different BtgZI sites may not be compatible.After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.BtgZI is typically used at 60°C, but is 75% active at 37°C. |
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Sticky ends from different PflMI sites may not be compatible. |
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Sticky ends from different StyI sites may not be compatible. |
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| * Blocked by Dam methylation. |
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Sticky ends from different BspQI sites may not be compatible. |
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Sticky ends from different SapI sites may not be compatible.SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot. |
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PciI is inhibited by nonionic detergents. |
| AmpR 2704 .. 3564 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 2: 2704 .. 3495 = 792 bp 263 amino acids = 28.9 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| AmpR 2704 .. 3564 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 1: signal sequence 3496 .. 3564 = 69 bp 23 amino acids = 2.6 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| AmpR 2704 .. 3564 = 861 bp 286 amino acids = 31.6 kDa 2 segments Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| ori 1945 .. 2533 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
| ori 1945 .. 2533 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
| TRE3G promoter 4 .. 382 = 379 bp 3rd-generation Tet-responsive promoter that can be activated by binding of Tet-On® 3G |
| TRE3G promoter 4 .. 382 = 379 bp 3rd-generation Tet-responsive promoter that can be activated by binding of Tet-On® 3G |
| ATG 423 .. 425 = 3 bp 1 amino acid = 149.2 Da Product: start codon |
| ATG 423 .. 425 = 3 bp 1 amino acid = 149.2 Da Product: start codon |
| DmrB 435 .. 755 = 321 bp 107 amino acids = 11.8 kDa Product: F36V mutant of FK506-binding protein FKBP12 binds synthetic ligands that do not bind wild-type FKBP |
| DmrB 435 .. 755 = 321 bp 107 amino acids = 11.8 kDa Product: F36V mutant of FK506-binding protein FKBP12 binds synthetic ligands that do not bind wild-type FKBP |
| SV40 poly(A) signal 1567 .. 1688 = 122 bp SV40 polyadenylation signal |
| SV40 poly(A) signal 1567 .. 1688 = 122 bp SV40 polyadenylation signal |
| AmpR promoter 3565 .. 3669 = 105 bp |
| AmpR promoter 3565 .. 3669 = 105 bp |
| small t intron 927 .. 992 = 66 bp simian virus 40 (SV40) small t antigen intron |
| small t intron 927 .. 992 = 66 bp simian virus 40 (SV40) small t antigen intron |
| 5' MCS 383 .. 422 = 40 bp multiple cloning site upstream of DmrB |
| 5' MCS 383 .. 422 = 40 bp multiple cloning site upstream of DmrB |
| MCS 756 .. 785 = 30 bp multiple cloning site |
| MCS 756 .. 785 = 30 bp multiple cloning site |
| tet operator 12 .. 30 = 19 bp bacterial operator O2 for the tetR and tetA genes |
| tet operator 12 .. 30 = 19 bp bacterial operator O2 for the tetR and tetA genes |
| tet operator 48 .. 66 = 19 bp bacterial operator O2 for the tetR and tetA genes |
| tet operator 48 .. 66 = 19 bp bacterial operator O2 for the tetR and tetA genes |
| tet operator 84 .. 102 = 19 bp bacterial operator O2 for the tetR and tetA genes |
| tet operator 84 .. 102 = 19 bp bacterial operator O2 for the tetR and tetA genes |
| tet operator 120 .. 138 = 19 bp bacterial operator O2 for the tetR and tetA genes |
| tet operator 120 .. 138 = 19 bp bacterial operator O2 for the tetR and tetA genes |
| tet operator 156 .. 174 = 19 bp bacterial operator O2 for the tetR and tetA genes |
| tet operator 156 .. 174 = 19 bp bacterial operator O2 for the tetR and tetA genes |
| tet operator 192 .. 210 = 19 bp bacterial operator O2 for the tetR and tetA genes |
| tet operator 192 .. 210 = 19 bp bacterial operator O2 for the tetR and tetA genes |
| tet operator 228 .. 246 = 19 bp bacterial operator O2 for the tetR and tetA genes |
| tet operator 228 .. 246 = 19 bp bacterial operator O2 for the tetR and tetA genes |
| ORF: 35 .. 301 = 267 bp ORF: 88 amino acids = 10.5 kDa |
| ORF: 2834 .. 3100 = 267 bp ORF: 88 amino acids = 9.2 kDa |
| ORF: 423 .. 788 = 366 bp ORF: 121 amino acids = 13.3 kDa |
| ORF: 1071 .. 1544 = 474 bp ORF: 157 amino acids = 18.2 kDa |
| ORF: 2704 .. 3564 = 861 bp ORF: 286 amino acids = 31.6 kDa |
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