pTRE3G-Hom1

Mammalian vector encoding a doxycycline-inducible FKBP homodimerizer domain, for creating soluble fusion proteins that can be dimerized with a drug. 

Sequence Author: Clontech (TaKaRa)

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EcoRI (3770) AatII (3699) ZraI (3697) XmnI (3376) ScaI (3257) TatI (3255) FspI (2999) NmeAIII (2925) BglI (2897) BsrFI (2857) BsaI (2838) AhdI (2777) AlwNI (2300) DrdI (1992) PaeR7I - PspXI - XhoI (1) HindIII (253) KasI (274) NarI (275) SfoI (276) PluTI (278) Eco53kI (292) SacI (294) SalI (383) AccI (384) PspOMI (393) ApaI (397) BglII (399) BspDI * - ClaI * (406) EagI (411) EcoRV (419) ATG PaqCI (431) BsmBI - Esp3I (457) TspMI - XmaI (550) SmaI (552) MluI (756) NdeI (763) NheI (768) BmtI (772) PstI - SbfI (778) BamHI (780) BtgZI (828) PflMI (1002) StyI (1150) BsaBI * (1465) MfeI (1553) HpaI (1566) BspQI - SapI (1768) PciI (1884) pTRE3G-Hom1 3775 bp
EcoRI  (3770)
1 site
G A A T T C C T T A A G
AatII  (3699)
1 site
G A C G T C C T G C A G
ZraI  (3697)
1 site
G A C G T C C T G C A G
XmnI  (3376)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (3257)
1 site
A G T A C T T C A T G A
TatI  (3255)
1 site
W G T A C W W C A T G W
FspI  (2999)
1 site
T G C G C A A C G C G T
NmeAIII  (2925)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BglI  (2897)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
BsrFI  (2857)
1 site
R C C G G Y Y G G C C R

Cleavage may be enhanced when more than one copy of the BsrFI recognition sequence is present.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
BsaI  (2838)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (2777)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (2300)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
DrdI  (1992)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
PaeR7I  (1)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (1)
1 site
V C T C G A G B B G A G C T C V
XhoI  (1)
1 site
C T C G A G G A G C T C
HindIII  (253)
1 site
A A G C T T T T C G A A
KasI  (274)
1 site
G G C G C C C C G C G G
NarI  (275)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (276)
1 site
G G C G C C C C G C G G
PluTI  (278)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
Eco53kI  (292)
1 site
G A G C T C C T C G A G
SacI  (294)
1 site
G A G C T C C T C G A G
SalI  (383)
1 site
G T C G A C C A G C T G
AccI  (384)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
PspOMI  (393)
1 site
G G G C C C C C C G G G
ApaI  (397)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
BglII  (399)
1 site
A G A T C T T C T A G A
BspDI  (406)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (406)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
EagI  (411)
1 site
C G G C C G G C C G G C
EcoRV  (419)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
PaqCI  (431)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the PaqCI recognition sequence.
Sticky ends from different PaqCI sites may not be compatible.
Cleavage can be improved with PaqCI Activator.
BsmBI  (457)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
Esp3I  (457)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different Esp3I sites may not be compatible.
TspMI  (550)
1 site
C C C G G G G G G C C C
XmaI  (550)
1 site
C C C G G G G G G C C C

Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (552)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
MluI  (756)
1 site
A C G C G T T G C G C A
NdeI  (763)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
NheI  (768)
1 site
G C T A G C C G A T C G
BmtI  (772)
1 site
G C T A G C C G A T C G
PstI  (778)
1 site
C T G C A G G A C G T C
SbfI  (778)
1 site
C C T G C A G G G G A C G T C C
BamHI  (780)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
BtgZI  (828)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
PflMI  (1002)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
StyI  (1150)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
BsaBI  (1465)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
MfeI  (1553)
1 site
C A A T T G G T T A A C
HpaI  (1566)
1 site
G T T A A C C A A T T G
BspQI  (1768)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (1768)
1 site
G C T C T