pGFP-C-shLenti

HuSH-29 lentiviral vector with chloramphenicol and puromycin resistance markers and a TurboGFP gene, for expressing an shRNA from the U6 promoter.

Sequence Author: OriGene

Try SnapGene for Free
|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Viral Expression & Packaging Vectors | More Plasmid Sets
No matches
HomePlasmidsViral Expression & Packaging VectorspGFP-C-shLenti
AclI (8255) BssSI - BssSαI (7346) DrdI (7281) Bsu36I (6597) BsrGI (5757) PmeI (5738) BsaBI * (5499) PaqCI (5487) BlpI (5210) PaeR7I - XhoI (5030) NotI (5024) MluI (5015) AscI (4978) MreI - SgrAI (4974) AsiSI (4972) CMV enhancer SgrDI (1) CMV enhancer EcoNI (1550) AleI (1957) KflI (2314) cPPT/CTS EcoRI (2618) BamHI (2951) BsmBI - Esp3I (3003) SphI (3087) NsiI (3089) SfiI (3220) AvrII (3267) PflFI - Tth111I (3338) BstEII (3430) CsiI - SexAI * (3863) XbaI (4271) pGFP-C-shLenti 8691 bp
AclI  (8255)
1 site		 A A C G T T T T G C A A
BssSI  (7346)
1 site		 C A C G A G G T G C T C
BssSαI  (7346)
1 site		 C A C G A G G T G C T C
DrdI  (7281)
1 site		 G A C N N N N N N G T C C T G N N N N N N C A G
Sticky ends from different DrdI sites may not be compatible.
Bsu36I  (6597)
1 site		 C C T N A G G G G A N T C C
Sticky ends from different Bsu36I sites may not be compatible.
BsrGI  (5757)
1 site		 T G T A C A A C A T G T
BsrGI is typically used at 37°C, but is even more active at 60°C.
PmeI  (5738)
1 site		 G T T T A A A C C A A A T T T G
BsaBI  (5499)
1 site		 G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
PaqCI  (5487)
1 site		 C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the PaqCI recognition sequence.
Sticky ends from different PaqCI sites may not be compatible.
Cleavage can be improved with PaqCI Activator.
BlpI  (5210)
1 site		 G C T N A G C C G A N T C G
Sticky ends from different BlpI sites may not be compatible.
PaeR7I  (5030)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (5030)
1 site		 C T C G A G G A G C T C
NotI  (5024)
1 site		 G C G G C C G C C G C C G G C G
MluI  (5015)
1 site		 A C G C G T T G C G C A
AscI  (4978)
1 site		 G G C G C G C C C C G C G C G G
MreI  (4974)
1 site		 C G C C G G C G G C G G C C G C
SgrAI  (4974)
1 site		 C R C C G G Y G G Y G G C C R C
Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
AsiSI  (4972)
1 site		 G C G A T C G C C G C T A G C G
SgrDI  (1)
1 site		 C G T C G A C G G C A G C T G C
EcoNI  (1550)
1 site		 C C T N N N N N A G G G G A N N N N N T C C
The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
AleI  (1957)
1 site		 C A C N N N N G T G G T G N N N N C A C
KflI  (2314)
1 site		 G G G W C C C C C C W G G G
Sticky ends from different KflI sites may not be compatible.
EcoRI  (2618)
1 site		 G A A T T C C T T A A G
BamHI  (2951)
1 site