pRSI17-U6-sh-HTS6-UbiC-TagGFP2-2A-Puro

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HomePlasmidsViral Expression & Packaging VectorspRSI17-U6-sh-HTS6-UbiC-TagGFP2-2A-Puro
MluI (1) SspI (7441) ScaI (7117) AhdI (6637) PspFI (6052) BseYI (6048) PciI (5744) SfiI (5322) NcoI (5276) AanI (5093) AanI (5037) KpnI (4702) Acc65I (4698) HincII (4103) SalI (4101) SexAI * (4062) SphI (96) NruI * (612) EcoNI (945) MfeI (964) BbvCI (1199) PpuMI - SanDI (1709) BspDI - ClaI (1798) BsaAI (1928) BbsI - BpiI (2064) BbsI - BpiI (2090) PaeR7I - PspXI - XhoI (2113) EcoRI (2280) AgeI (2289) BstBI (2298) AfeI (2358) XbaI (2702) BsaBI * (3300) BamHI (3429) PflFI - Tth111I (3537) BsiWI (3551) BspEI * (3608) RsrII (3611) MscI * (3870) EagI (3943) pRSI17-U6-sh-HTS6- UbiC-TagGFP2-2A-Puro 8028 bp
MluI  (1)
1 site		 A C G C G T T G C G C A
SspI  (7441)
1 site		 A A T A T T T T A T A A
ScaI  (7117)
1 site		 A G T A C T T C A T G A
AhdI  (6637)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
PspFI  (6052)
1 site		 C C C A G C G G G T C G
BseYI  (6048)
1 site		 C C C A G C G G G T C G
After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
PciI  (5744)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
SfiI  (5322)
1 site		 G G C C N N N N N G G