pBABE-Puro

Retroviral expression vector derived from the Moloney murine leukemia virus. Contains a puromycin resistance marker.

Sequence Author: Cell Biolabs

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SspI (5058) XmnI (4853) ScaI (4734) PvuI (4624) FspI (4476) NotI (4133) DrdI (3462) AflIII - PciI (3354) BspQI - SapI (3238) BbsI (3102) BmtI (2484) BfuAI - BspMI - PaqCI (8) SpeI (616) Bsu36I (737) BsrGI (1230) NgoMIV (1348) NaeI (1350) BamHI (1355) BsaAI - SnaBI (1374) EcoRI (1379) SalI (1397) AccI (1398) NcoI (1634) SfiI (1680) AvrII (1727) HindIII (1743) BsiWI (1808) BspEI * (1865) RsrII (1868) SacII (1966) BspDI - ClaI (2406) NheI (2480) pBABE-Puro 5169 bp
SspI  (5058)
1 site
A A T A T T T T A T A A
XmnI  (4853)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (4734)
1 site
A G T A C T T C A T G A
PvuI  (4624)
1 site
C G A T C G G C T A G C
FspI  (4476)
1 site
T G C G C A A C G C G T
NotI  (4133)
1 site
G C G G C C G C C G C C G G C G
DrdI  (3462)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
AflIII  (3354)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (3354)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BspQI  (3238)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (3238)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
BbsI  (3102)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BmtI  (2484)
1 site
G C T A G C C G A T C G
BfuAI  (8)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (8)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
PaqCI  (8)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the PaqCI recognition sequence.
Sticky ends from different PaqCI sites may not be compatible.
Cleavage can be improved with PaqCI Activator.
SpeI  (616)
1 site
A C T A G T T G A T C A
Bsu36I  (737)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
BsrGI  (1230)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
NgoMIV  (1348)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
NaeI  (1350)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
BamHI  (1355)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
BsaAI  (1374)
1 site
Y A C G T R R T G C A Y
SnaBI  (1374)
1 site
T A C G T A A T G C A T
EcoRI  (1379)
1 site
G A A T T C C T T A A G
SalI  (1397)
1 site
G T C G A C C A G C T G
AccI  (1398)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
NcoI  (1634)
1 site
C C A T G G G G T A C C
SfiI  (1680)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
AvrII  (1727)
1 site
C C T A G G G G A T C C
HindIII  (1743)
1 site
A A G C T T T T C G A A
BsiWI  (1808)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
BspEI  (1865)
1 site
T C C G G A A G G C C T
* Blocked by Dam methylation.
RsrII  (1868)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
SacII  (1966)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
BspDI  (2406)
1 site
A T C G A T T A G C T A
ClaI  (2406)
1 site
A T C G A T T A G C T A
NheI  (2480)
1 site
G C T A G C C G A T C G
AmpR
4181 .. 5041  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
   Segment 2:  
   4181 .. 4972  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4181 .. 5041  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
   Segment 1:  signal sequence  
   4973 .. 5041  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4181 .. 5041  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
PuroR
1752 .. 2351  =  600 bp
199 amino acids  =  21.5 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
PuroR
1752 .. 2351  =  600 bp
199 amino acids  =  21.5 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
LTR
2450 .. 3043  =  594 bp
long terminal repeat from Moloney murine leukemia virus
LTR
2450 .. 3043  =  594 bp
long terminal repeat from Moloney murine leukemia virus
ori
3415 .. 4003  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
3415 .. 4003  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
LTR (truncated)
8 .. 479  =  472 bp
long terminal repeat from Moloney murine leukemia virus
LTR (truncated)
8 .. 479  =  472 bp
long terminal repeat from Moloney murine leukemia virus
gag (truncated)
934 .. 1350  =  417 bp
truncated MMLV gag gene lacking the start codon
gag (truncated)
934 .. 1350  =  417 bp
truncated MMLV gag gene lacking the start codon
SV40 promoter
1413 .. 1742  =  330 bp
SV40 enhancer and early promoter
SV40 promoter
1413 .. 1742  =  330 bp
SV40 enhancer and early promoter
MMLV Ψ
542 .. 741  =  200 bp
packaging signal of Moloney murine leukemia virus (MMLV)
MMLV Ψ
542 .. 741  =  200 bp
packaging signal of Moloney murine leukemia virus (MMLV)
AmpR promoter
5042 .. 5146  =  105 bp
AmpR promoter
5042 .. 5146  =  105 bp
MCS
1355 .. 1402  =  48 bp
multiple cloning site
MCS
1355 .. 1402  =  48 bp
multiple cloning site
splice donor mutation
541 .. 541  =  1 bp
T-to-C mutation that blocks RNA splicing
splice donor mutation
541 .. 541  =  1 bp
T-to-C mutation that blocks RNA splicing
SV40 ori
1593 .. 1728  =  136 bp
SV40 origin of replication
SV40 ori
1593 .. 1728  =  136 bp
SV40 origin of replication
ORF:  1752 .. 2351  =  600 bp
ORF:  199 amino acids  =  21.5 kDa
ORF:  4311 .. 4577  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  1728 .. 2402  =  675 bp
ORF:  224 amino acids  =  22.9 kDa
ORF:  4181 .. 5041  =  861 bp
ORF:  286 amino acids  =  31.5 kDa
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