pLVX-TRE3G-IRES

Lentiviral vector for doxycycline-inducible expression of two genes in cells containing the Tet-On 3G transactivator protein.

Sequence Author: Clontech (TaKaRa)

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Explore Over 2.7k Plasmids: Viral Expression & Packaging Vectors | More Plasmid Sets
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HomePlasmidsViral Expression & Packaging VectorspLVX-TRE3G-IRES
XcmI (8267) SgrDI (8042) SspI (7925) PvuI (7491) FspI (7343) AhdI (7121) DrdI (6339) BmtI (5847) NheI (5843) Bsu36I (5053) NcoI (4740) CsiI - SexAI * (4282) NruI * (833) EcoNI (1170) MfeI (1189) BbvCI (1424) AleI (1577) KflI (1934) BspDI - ClaI (2180) PaeR7I - PspXI - XhoI (2203) BamHI (2556) MCS 1 NotI (2569) SfiI (2577) AvrII (2736) PaqCI (2924) TspMI - XmaI (3166) SmaI (3168) MluI (3173) NdeI (3180) EcoRI (3185) SphI (3234) AgeI (3305) SpeI (3440) BlpI (3468) PflFI - Tth111I (3757) PasI (3764) BsiWI (3771) RsrII (3831) BstEII (3849) MscI * (4090) pLVX-TRE3G-IRES 8410 bp
XcmI  (8267)
1 site		 C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C
The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
SgrDI  (8042)
1 site		 C G T C G A C G G C A G C T G C
SspI  (7925)
1 site		 A A T A T T T T A T A A
PvuI  (7491)
1 site		 C G A T C G G C T A G C
FspI  (7343)
1 site		 T G C G C A A C G C G T
AhdI  (7121)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
DrdI  (6339)
1 site		 G A C N N N N N N G T C C T G N N N N N N C A G
Sticky ends from different DrdI sites may not be compatible.
BmtI  (5847)
1 site		 G C T A G C C G A T C G
NheI  (5843)
1 site		 G C T A G C C G A T C G
Bsu36I  (5053)
1 site		 C C T N A G G G G A N T C C
Sticky ends from different Bsu36I sites may not be compatible.
NcoI  (4740)
1 site		 C C A T G G G G T A C C
CsiI  (4282)
1 site		 A C C W G G T T G G W C C A
Sticky ends from different CsiI sites may not be compatible.
SexAI  (4282)
1 site		 A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
NruI  (833)
1 site		 T C G C G A A G C G C T
* Blocked by Dam methylation.
EcoNI  (1170)
1 site		 C C T N N N N N A G G G G A N N N N N T C C
The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
MfeI  (1189)
1 site		 C A A T T G G T T A A C
BbvCI  (1424)
1 site		 C C T C A G