pLNCX2

Retroviral vector for transient or stable constitutive expression of a gene.

Sequence Author: Clontech (TaKaRa)

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HomePlasmidsViral Expression & Packaging VectorspLNCX2
SacII (6112) SspI (5800) XmnI (5595) ScaI (5476) PvuI (5366) AseI (5168) AflIII - PciI (4103) BstZ17I (3876) XbaI (3331) 3' pLNCX Seq/PCR primer (3031 .. 3056) AscI (443) PshAI (577) SpeI (726) BstEII (1153) CsiI - SexAI * (1281) BsrGI (1348) BclI * (1481) BsaBI * (1499) SphI (2045) NgoMIV (2140) NaeI (2142) RsrII (2156) BstBI (2322) CMV enhancer SnaBI (2656) 5' pLNCX Seq/PCR primer (2881 .. 2905) BglII (2925) PaeR7I - XhoI (2929) HindIII (2938) NotI (2956) SalI (2965) StuI (2973) BspDI - ClaI (2990) pLNCX2 6132 bp
SacII  (6112)
1 site		 C C G C G G G G C G C C
Efficient cleavage requires at least two copies of the SacII recognition sequence.
SspI  (5800)
1 site		 A A T A T T T T A T A A
XmnI  (5595)
1 site		 G A A N N N N T T C C T T N N N N A A G
ScaI  (5476)
1 site		 A G T A C T T C A T G A
PvuI  (5366)
1 site		 C G A T C G G C T A G C
AseI  (5168)
1 site		 A T T A A T T A A T T A
AflIII  (4103)
1 site		 A C R Y G T T G Y R C A
Sticky ends from different AflIII sites may not be compatible.
PciI  (4103)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
BstZ17I  (3876)
1 site		 G T A T A C C A T A T G
XbaI  (3331)
1 site		 T C T A G A A G A T C T
AscI  (443)
1 site		 G G C G C G C C C C G C G C G G
PshAI  (577)
1 site		 G A C N N N N G T C C T G N N N N C A G
PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
SpeI  (726)
1 site		 A C T A G T T G A T C A
BstEII  (1153)
1 site		 G G T N A C C C C A N T G G
Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
CsiI  (1281)
1 site		 A C C W G G T T G G W C C A
Sticky ends from different CsiI sites may not be compatible.
SexAI  (1281)
1 site		 A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
BsrGI  (1348)
1 site		 T G T A C A A C A T G T
BsrGI is typically used at 37°C, but is even more active at 60°C.
BclI  (1481)
1 site		 T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BsaBI  (1499)
1 site		 G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
SphI  (2045)
1 site		 G C A T G C C G T A C G
NgoMIV  (2140)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
NaeI  (2142)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NaeI recognition sequence.
RsrII  (2156)
1 site		 C G G W C C G G C C W G G C
Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BstBI  (2322)
1 site		 T T C G A A A A G C T T
SnaBI  (2656)
1 site		 T A C G T A A T G C A T
BglII  (2925)
1 site		 A G A T C T T C T A G A
PaeR7I  (2929)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (2929)
1 site		 C T C G A G G A G C T C
HindIII  (2938)
1 site		 A A G C T T T T C G A A
NotI  (2956)
1 site		 G C G G C C G C C G C C G G C G
SalI  (2965)
1 site		 G T C G A C C A G C T G
StuI  (2973)
1 site		 A G G C C T T C C G G A
BspDI  (2990)
1 site		 A T C G A T T A G C T A
ClaI  (2990)
1 site		 A T C G A T T A G C T A
3' pLNCX Seq/PCR primer
26-mer  /  54% GC
1 binding site
3031 .. 3056  =  26 annealed bases
Tm  =  64°C
5' pLNCX Seq/PCR primer
25-mer  /  48% GC
1 binding site
2881 .. 2905  =  25 annealed bases
Tm  =  61°C
AmpR
4923 .. 5783  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
   Segment 2:  
   4923 .. 5714  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4923 .. 5783  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
   Segment 1:  signal sequence  
   5715 .. 5783  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4923 .. 5783  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
NeoR/KanR
1512 .. 2306  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin)
NeoR/KanR
1512 .. 2306  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin)
3' LTR
3034 .. 3627  =  594 bp
long terminal repeat from Moloney murine leukemia virus
3' LTR
3034 .. 3627  =  594 bp
long terminal repeat from Moloney murine leukemia virus
5' LTR
1 .. 589  =  589 bp
long terminal repeat from Moloney murine sarcoma virus
5' LTR
1 .. 589  =  589 bp
long terminal repeat from Moloney murine sarcoma virus
ori
4164 .. 4752  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
4164 .. 4752  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
gag (truncated)
1052 .. 1468  =  417 bp
truncated MMLV gag gene lacking the start codon
gag (truncated)
1052 .. 1468  =  417 bp
truncated MMLV gag gene lacking the start codon
CMV enhancer
2377 .. 2680  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
2377 .. 2680  =  304 bp
human cytomegalovirus immediate early enhancer
CMV promoter
2681 .. 2884  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
2681 .. 2884  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
MMLV Ψ
652 .. 851  =  200 bp
packaging signal of Moloney murine leukemia virus (MMLV)
MMLV Ψ
652 .. 851  =  200 bp
packaging signal of Moloney murine leukemia virus (MMLV)
AmpR promoter
5784 .. 5888  =  105 bp
AmpR promoter
5784 .. 5888  =  105 bp
MCS
2925 .. 2994  =  70 bp
multiple cloning site
MCS
2925 .. 2994  =  70 bp
multiple cloning site
ORF:  1684 .. 2070  =  387 bp
ORF:  128 amino acids  =  14.6 kDa
ORF:  5053 .. 5319  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  1512 .. 2306  =  795 bp
ORF:  264 amino acids  =  29.0 kDa
ORF:  739 .. 999  =  261 bp
ORF:  86 amino acids  =  9.5 kDa
ORF:  3451 .. 3741  =  291 bp
ORF:  96 amino acids  =  10.5 kDa
ORF:  1821 .. 2075  =  255 bp
ORF:  84 amino acids  =  9.6 kDa
ORF:  4923 .. 5783  =  861 bp
ORF:  286 amino acids  =  31.5 kDa
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Individual Sequences & Maps

pAAV-AC-GFP
pLenti6.2 N-Lumio V5-DEST
pLVX-rHom-1
pRetroX-Tet3G
pAAV-AC-Myc-DDK
pLenti6.2 V5-DEST
pLVX-rHom-Nuc1
pRetroX-TetOne
pAAV-AC-RFP
pLenti6.3 TO V5-DEST
pLVX-rHom-Sec1
pRetroX-TetOne-Puro
pAAV2-EF1a-tGFP-WPRE
pLenti6.3 V5-DEST
pLVX-Tet-Off Advanced
pRetroX-Tight-Hyg
pAd BLOCK-iT-DEST
pLenti6.4 R4R2 V5-DEST
pLVX-Tet-On Advanced
pRetroX-Tight-Pur
pAd CMV V5-DEST
pLenti6 BLOCK-iT-DEST
pLVX-Tet3G
pRetroX-TRE3G
pAd PL-DEST
pLenti6 capTEV-CT-DEST
pLVX-TetOne
pRFP-C-RS
pAdenoX-CMV Linear
pLenti6 capTEV-NT-DEST1
pLVX-TetOne-Puro
pRFP-CB-shLenti
pAdenoX-DsRed-Express Linear
pLenti6 TR
pLVX-Tight-Puro
pRS
pAdenoX-PRLS Linear
pLenti6 UbC V5-DEST
pLVX-TRE3G
pRSGC1-U6-sg-HTS6-CMV-Cas9-2A-Puro
pAdenoX-PRLS-DsRed-Express Linear
pLenti6 V5-DEST
pLVX-TRE3G-Hom1
pRSGC2-U6-sg-HTS6-UbiC-Cas9-2A-Puro
pAdenoX-PRLS-ZsGreen1 Linear
pLenti7.3 V5-DEST
pLVX-TRE3G-IRES
pRSI12-U6-sh-HTS4-UbiC-TagRFP-2A-Puro
pAdenoX-Tet3G Linear
pLEX-MCS
pLVX-TRE3G-mCherry
pRSI16-U6-sh-HTS6-UbiC-TagRFP-2A-Puro
pAdenoX-ZsGreen1 Linear
pLHCX
pLVX-TRE3G-ZsGreen1
pRSI16cb-U6-sh-13kCB18-HTS6-UbiC-TagRFP-2A-Puro
pB-RS
pLKO.1
pLX301
pRSI17-U6-sh-HTS6-UbiC-TagGFP2-2A-Puro
pBABE-Hygro
pLKO.1 puro
pLX302
pRSI17cb-U6-sh-13kCB18-HTS6-UbiC-TagGFP2-2A-Puro
pBABE-Neo
pLMN-ZsGreen-Neomycin
pLX303
pRSIT12-U6Tet-sh-HTS4-CMV-TetRep-2A-TagRFP-2A-Puro
pBABE-Puro
pLMPd-Ametrine
pLX304
pRSIT16-U6Tet-sh-HTS6-CMV-TetRep-2A-TagRFP-2A-Puro
pBABE-Zeo
pLNCX2
pLX304-V5-Blast-Empty
pRSIT17-U6Tet-sh-HTS6-CMV-TetRep-2A-TagGFP2-2A-Puro
pCMV-VSV-G
pLP VSVG
pLXIN
pRSITEP-U6Tet-sh-noHTS-EF1-TetRep-2A-Puro
pGFP-B-RS
pLP1
pLXSN
pRSITUR-U6Tet-sh-HTS3-UbiC-TetRep-2A-TagRFP
pGFP-C-shLenti
pLP2
pMD2.G
pShuttle2
pGFP-V-RS
pLPCX
pMSCV16URP-U6-sh-HTS6-UbiC-TagRFP-2A-Puro
pSIREN-RetroQ
pGIPZ
pLVX-EF1alpha-IRES-mCherry
pMSCVhyg
pSIREN-RetroQ-DsRed-Express
pLenti-C-GFP
pLVX-EF1alpha-IRES-Puro
pMSCVneo
pSIREN-RetroQ-TetH
pLenti-C-mGFP
pLVX-EF1alpha-IRES-ZsGreen1
pMSCVpuro
pSIREN-RetroQ-TetP
pLenti-C-Myc-DDK
pLVX-EF1alpha-Tet3G
pQC-tTS-IN
pSIREN-RetroQ-ZsGreen1
pLenti-C-Myc-DDK-IRES-BSD
pLVX-Het-1
pQCXIH
psPAX2
pLenti-C-Myc-DDK-IRES-GFP
pLVX-Het-2
pQCXIN
pTRE3G-Hom1
pLenti-C-Myc-DDK-IRES-Neo
pLVX-Het-Mem1
pQCXIP
pTRIPZ
pLenti-C-Myc-DDK-IRES-Puro
pLVX-Het-Nuc1
pQCXIX
ptTS-Neo
pLenti-C-Myc-DDK-IRES-tRFP
pLVX-Hom-1
pRetro-Lib
pZIP-hCMV-RFP-Puro
pLenti-C-tRFP
pLVX-Hom-Mem1
pRetroQ-AcGFP1-C1
pZIP-hCMV-ZSGreen-Puro
pLenti-C-tYFP
pLVX-Hom-Nuc1
pRetroQ-AcGFP1-N1
pZIP-hEF1-alpha-RFP-Puro
pLenti-EF1a-C-mGFP
pLVX-IRES-Hyg
pRetroQ-DsRed-Monomer-C1
pZIP-hEF1-alpha-ZsGreen-Puro
pLenti-EF1a-C-Myc-DDK-IRES-Puro
pLVX-IRES-mCherry
pRetroQ-DsRed-Monomer-N1
pZIP-mCMV-RFP-Puro
pLenti-EF1a-C-tGFP
pLVX-IRES-Neo
pRetroQ-mCherry-C1
pZIP-mCMV-ZsGreen-Puro
pLenti-N-tGFP
pLVX-IRES-Puro
pRetroQ-mCherry-N1
pZIP-mEF1-alpha-RFP-Puro
pLenti-N-tRFP
pLVX-IRES-tdTomato
pRetroX-IRES-DsRedExpress
pZIP-mEF1-alpha-ZsGreen-Puro
pLenti-N-tYFP
pLVX-IRES-ZsGreen1
pRetroX-IRES-ZsGreen1
pZIP-SFFV-RFP-Puro
pLenti3.3 TR
pLVX-PTuner
pRetroX-PTuner
pZIP-SFFV-ZsGreen-Puro
pLenti4 BLOCK-iT-DEST
pLVX-PTuner Green
pRetroX-PTuner IRES
pZIP-TRE3G-ZsGreen-Puro
pLenti4 V5-DEST
pLVX-PTunerC
pRetroX-Tet-Off Advanced
pSIREN-Shuttle (linearized)
pLenti6.2 C-Lumio V5-DEST
pLVX-Puro
pRetroX-Tet-On Advanced
scramble shRNA

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Lucigen Vectors
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