pLEX-MCS

Empty lentiviral expression vector containing a multiple cloning site.

Sequence Author: Open Biosystems

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AmpR promoter SspI (10,245) FspI (9663) AhdI (9441) BstZ17I (8169) AsiSI (7246) PshAI (6909) PmeI (5770) SgrDI (10,362) NruI * (833) EcoNI (1170) MfeI (1189) BbvCI - Bpu10I (1424) AleI (1577) KflI - PpuMI (1934) SgrAI (2500) MauBI (2578) SfiI (2621) SnaBI (3070) CMV promoter pLEX-MCS-FWD (3197 .. 3216) BamHI (3378) SpeI (3384) MCS NotI (3428) PaeR7I - PspXI - XhoI (3434) MluI (3453) AgeI (3465) pLEX-MCS-REV (3524 .. 3547) PaqCI (3812) BsiWI (4107) BstEII (4185) HpaI (4653) Bsu36I (5377) pLEX-MCS 10,682 bp
SspI  (10,245)
1 site
A A T A T T T T A T A A
FspI  (9663)
1 site
T G C G C A A C G C G T
AhdI  (9441)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BstZ17I  (8169)
1 site
G T A T A C C A T A T G
AsiSI  (7246)
1 site
G C G A T C G C C G C T A G C G
PshAI  (6909)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
PmeI  (5770)
1 site
G T T T A A A C C A A A T T T G
SgrDI  (10,362)
1 site
C G T C G A C G G C A G C T G C
NruI  (833)
1 site
T C G C G A A G C G C T
* Blocked by Dam methylation.
EcoNI  (1170)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
MfeI  (1189)
1 site
C A A T T G G T T A A C
BbvCI  (1424)
1 site
C C T C A G C G G A G T C G
Bpu10I  (1424)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
AleI  (1577)
1 site
C A C N N N N G T G G T G N N N N C A C
KflI  (1934)
1 site
G G G W C C C C C C W G G G

Sticky ends from different KflI sites may not be compatible.
PpuMI  (1934)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
SgrAI  (2500)
1 site
C R C C G G Y G G Y G G C C R<